Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,203,428	A→G	S37G (AGC→GGC)	zraR →	fused DNA‑binding response regulator in two‑component regulatory system with ZraS: response regulator/sigma54 interaction protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,203,428	0	A	G	100.0%	146.8 / NA	44	S37G (AGC→GGC)	zraR	fused DNA‑binding response regulator in two‑component regulatory system with ZraS: response regulator/sigma54 interaction protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/23);  total (21/23)

GGCTTCCGGTCAATATTACGCGTAAGGACCCACAAGGATGACGCACGATAATATCGATATTCTGGTGGTGGATGATGACATTAGCCACTGCACTATTTTGCAGGCTTTACTGCGCGGCTGGGGCTATAACGTCGCGCTGGCGAACAGCGGGCGACAGGCGTTGGAGCAGGTGCGGGAACAGGTTTTTGATCTTGTGCTTTGCGATGTGCGAATGGCGGAGATGGACGGCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGT  >  NC_000913/4203283‑4203575                                                                                                                                                  |                                                                                                                                                    ggCTTCCGGTCAATATTACGCGTAAGGACCCACAAGGATGACGCACGATAATATCGATATTCTGGTGGTGGATGATGACATTAGCCACTGCACTATTTTGCAGGCTTTACTGCGCGGCTGGGGCTATAACGTCGCGCTGGCGAACGGCg                                                                                                                                                  <  1:343200/149‑1 (MQ=255)      ccGGTCAATATTACGCGTAAGGACCCACAAGGATGACGCACGATAATATCGATATTCTGGTGGTGGATGATGACATTAGCCACTGCACTATTTTGCAGGCTTTACTGCGCGGCTGGGGCTATAACGTCGCGCTGGCGAACGGCGGGCGACa                                                                                                                                           <  2:685362/151‑1 (MQ=255)            aaTATTACGCGTAAGGACCCACAAGGATGACGCACGATAATATCGATATTCTGGTGGTGGATGATGACATTAGCCACTGCACTATTTTGCAGGCTTTACTGCGCGGCTGGGGCTATAACGTCGCGCTGGCGAACGGCGGGCGACAGGCGtt                                                                                                                                     <  1:598215/151‑1 (MQ=255)             atatTACGCGTAAGGACCCACAAGGATGACGCACGATAATATCGATATTCTGGTGGTGGATGATGACATTAGCCACTGCACTATTTTGCAGGCTTTACTGCGCGGCTGGGGCTATAACGTCGCGCTGGCGAACGGCGGGCGACAGGCGTTg                                                                                                                                    >  1:160502/1‑151 (MQ=255)             atatTACGCGTAAGGACCCACAAGGATGACGCACGATAATATCGATATTCTGGTGGTGGATGATGACATTAGCCACTGCACTATTTTGCAGGCTTTACTGCGCGGCCGGGGCTATAACGTCGCGCTGGCGAACGGCGGGCGACAGGCGTTg                                                                                                                                    >  1:345608/1‑151 (MQ=255)                      gTAAGGACCCACAAGGATGACGCACGATAATATCGATATTCTGGTGGTGGATGATGACATTAGCCACTGCACTATTTTGCAGGCTTTACTGCGCGGCTGGGGCTATAACGTCGCGCTGGCGAACGGCGGGCGACAGGCGTTGGAGCAGGTg                                                                                                                           <  1:123703/151‑1 (MQ=255)                        aaGGACCCACAAGGATGACGCACGATAATATCGATATTCTGGTGGTGGATGATGACATTAGCCACTGCACTATTTTGCAGGCTTTACTGCGCGGCTGGGGCTATAACGTCGCGCTGGCGAACGGCGGGCGACAGGCGTTGGAGCAGGTGc                                                                                                                          <  2:751440/150‑1 (MQ=255)                        aaGGACCCACAAGGATGACGCACGATAATATCGATATTCTGGTGGTGGATGATGACATTAGCCACTGCACTATTTTGCAGGCTTTACTGCGCGGCTGGGGCTATAACGTCGCGCTGGCGAACGGCGGGCGACAGGCGTTGGAGCAGGTGCg                                                                                                                         >  2:555183/1‑151 (MQ=255)                             cccACAAGGATGACGCACGATAATATCGATATTCTGGTGGTGGATGATGACATTAGCCACTGCACTATTTTGCAGGCTTTACTGCGCGGCTGGGGCTATAACGTCGCGCTGGCGAACGGCGGGCGACAGGCGTTGGAGCAGGTGCGGGAAc                                                                                                                    <  2:178437/151‑1 (MQ=255)                               cacaAGGATGACGCACGATAATATCGATATTCTGGTGGTGGATGATGACATTAGCCACTGCACTATTTTGCAGGCTTTACTGCGCGGCTGGGGCTATAACGTCGCGCTGGCGAACGGCGGGCGACAGGCGTTGGAGCAGGTGCGGGaa                                                                                                                     >  1:525226/1‑148 (MQ=255)                                         aCGCACGATAATATCGATATTCTGGTGGTGGATGATGACATTAGCCACTGCACTATTTTGCAGGCTTTACTGCGCGGCTGGGGCTATAACGTCGCGCTGGCGAACGGCGGGCGACAGGCGTTGGAGCAGGTGCGGGAACAGGTTTTTGAt                                                                                                         >  1:434354/1‑150 (MQ=255)                                         aCGCACGATAATATCGATATTCTGGTGGTGGATGATGACATTAGCCACTGCACTATTTTGCAGGCTTTACTGCGCGGCTGGGGCTATAACGTCGCGCTGGCGAACGGCGGGCGACAGGCGTTGGAGCAGGTGCGGGAACAGGTTTTTGATc                                                                                                        >  2:156921/1‑151 (MQ=255)                                            cACGATAATATCGATATTCTGGTGGTGGATGATGACATTAGCCACTGCACTATTTTGCAGGCTTTACTGCGCGGCTGGGGCTATAACGTCGCGCTGGCGAACGGCGGGCGACAGGCGTTGGAGCAGGTGCGGGAACAGGTTTTTGATCTtg                                                                                                     <  1:33712/151‑1 (MQ=255)                                             aCGATAATATCGATATTCTGGTGGTGGATGATGACATTAGCCACTGCACTATTTTGCAGGCTTTACTGCGCGGCTGGGGCTATAACGTCGCGCTGGCGAACGGCGGGCGACAGGCGTTGGAGCAGGTGCGGGAACAGGTTTTTGATCTtgt                                                                                                    >  1:240515/1‑151 (MQ=255)                                                     atCGATATTCTGGTGGTGGATGATGACATTAGCCACTGCACTATTTTGCAGGCTTTACTGCGCGGCTGGGGCTATAACGTCGCGCTGGCGAACGGCGGGCGACAGGCGTTGGAGCAGGTGCGGGAACAGGTTTTTGATCTTGTGCTTTGc                                                                                             >  2:611591/1‑150 (MQ=255)                                                     atCGATATTCTGGTGGTGGATGATGACATTAGCCACTGCACTATTTTGCAGGCTTTACTGCGCGGCTGGGGCTATAACGTCGCGCTGGCGAACGGCGGGCGACAGGCGTTGGAGCAGGTGCGGGAACAGGTTTTTGATCTTGTGCTTTGc                                                                                             <  2:277954/150‑1 (MQ=255)                                                     atCGATATTCTGGTGGTGGATGATGACATTAGCCACTGCACTATTTTGCAGGCTTTACTGCGCGGCTGGGGCTATAACGTCGCGCTGGCGAACGGCGGGCGACAGGCGTTGGAGCAGGTGCGGGAACAGGTTTTTGATCTTGTGCTTTGCg                                                                                            >  1:452554/1‑151 (MQ=255)                                                     atCGATATTCTGGTGGTGGATGATGACATTAGCCACTGCACTATTTTGCAGGCTTTACTGCGCGGCTGGGGCTATAACGTCGCGCTGGCGAACGGCGGGCGACAGGCGTTGGAGCAGGTGCGGGAACAGGTTTTTGATCTTGTGCTTTGCg                                                                                            <  2:112185/151‑1 (MQ=255)                                                          tatTCTGGTGGTGGATGATGACATTAGCCACTGCACTATTTTGCAGGCTTTACTGCGCGGCTGGGGCTATAACGTCGCGCTGGCGAACGGCGGGCGACAGGCGTTGGAGCAGGTGCGGGAACAGGTTTTTGATCTTGTGCTTTACGAtgtg                                                                                       >  2:430471/1‑151 (MQ=255)                                                           atTCTGGTGGTGGATGATGACATTAGCCACTGCACTATTTTGCAGGCTTTACTGCGCGGCTGGGGCTATAACGTCGCGCTGGCGAACGGCGGGCGACAGGCGTTGGAGCAGGTGCGGGAACAGGTTTTTGATCTTGTGCTTTGCGATGTGc                                                                                      >  2:248803/1‑151 (MQ=255)                                                            ttCTGGTGGTGGATGATGACATTAGCCACTGCACTATTTTGCAGGCTTTACTGCGCGGCTGGGGCTATAACGTCGCGCTGGCGAACGGCGGGCGACAGGCGTTGGAGCAGGTGCGGGAACAGGTTTTTGATCTTGTGCTTTGCGATGTGCg                                                                                     >  2:66891/1‑151 (MQ=255)                                                                     tggATGATGACATTAGCCACTGCACTATTTTGCAGGCTTTACTGCGCGGCTGGGGCTATAACGTCGCGCTGGCGAACGGCGGGCGACAGGCGTTGGAGCAGGTGCGGGAACAGGTTTTTGATCTTGTGCTTTGCGATGTGCGAATGGCgg                                                                             >  1:408061/1‑150 (MQ=255)                                                                              aCATTAGCCACTGCACTATTTTGCAGGCTTTACTGCGCGGCTGGGGCTATAACGTCGCGCTGGCGAACGGCGGGCGACAGGCGTTGGAGCAGGTGCGGGAACAGGTTTTTGATCTTGTGCTTTGCGATGTGCGAATGGCGGAGATGGACgg                                                                   <  2:140782/151‑1 (MQ=255)                                                                                  tAGCCACTGCACTATTTTGCAGGCTTTACTGCGCGGCTGGGGCTATAACGTCGCGCTGGCGAACGGCGGGCGACAGGCGTTGGAGCAGGTGCGGGAACAGGTTTTTGATCTTGTGCTTTGCGATGTGCGAATGGCGGAGATGGACGGCAt                                                                <  2:718872/150‑1 (MQ=255)                                                                                   aGCCACTGCACTATTTTGCAGGCTTTACTGCGCGGCTGGGGCTATAACGTCGCGCTGGCGAACGGCGGGCGACAGGCGTTGGAGCAGGTGCGGGAACAGGTTTTTGATCTTGTGCTTTGCGATGTGCGAATGGCGGAGATGGACGGCATCg                                                              <  1:343425/151‑1 (MQ=255)                                                                                     ccACTGCACTATTTTGCAGGCTTTACTGCGCGGCTGGGGCTATAACGTCGCGCTGGCGAACGGCGGGCGACAGGCGTTGGAGCAGGTGCGGGAACAGGTTTTTGATCTTGTGCTTTGCGATGTGCGAATGGCGGAGATGGACGGCATCGcc                                                            <  1:299732/151‑1 (MQ=255)                                                                                        cTGCACTATTTTGCAGGCTTTACTGCGCGGCTGGGGCTATAACGTCGCGCTGGCGAACGGCGGGCGACAGGCGTTGGAGCAGGTGCGGGAACAGGTTTTTGATCTTGTGCTTTGCGATGTGCGAATGGCGGAGATGGACGGCATCGCCACg                                                         >  2:361985/1‑151 (MQ=255)                                                                                           caatATTTTGCAGGCTTTACTGCGCGGCTGGGGCTATAACGTCGCGCTGGCGAACGGCGGGCGACAGGCGTTGGAGCAGGTGCGGGAACAGGTTTTTGATCTTGTGCTTTGCGATGTGCGAATGGCGGAGATGGACGGCATCGCCACGCTg                                                      <  1:283711/148‑1 (MQ=255)                                                                                              tATTTTGCAGGCTTTACTGCGCGGCTGGGGCTATAACGTCGCGCTGGCGAACGGCGGGCGACAGGCGTTGGAGCAGGTGCGGGAACAGGTTTTTGATCTTGTGCTTTGCGATGTGCGAATGGCGGAGATGGACGGCATCGCCACGCTGaa                                                    >  1:451399/1‑150 (MQ=255)                                                                                                  ttGCAGGCTTTACTGCGCGGATGGGGCTAGAACGTCGCGCTGGCGAACGGCGGGCGACAGGCGTTGGAGCAGGTGCGGGAACAGTTTGTTGATCGTGTGCTTTGCGATGGGAGAATGGCGGAGATGGACGGCATCGCCATACTTAAagag                                                <  1:698832/150‑1 (MQ=255)                                                                                                    gCAGGCTTTACTGCGCGGCTGGGGCTATAACGTCGCGCTGGCGAACGGCGGGCGACAGGCGTTGGAGCAGGTGCGGGAACAGGTTTTTGATCTTGTGCTTTGCGATGTGCGAATGGCGGAGATGGACGGCATCGCCACGCTGAAAGAGAt                                              <  1:361939/150‑1 (MQ=255)                                                                                                       ggCTTTACTGCGCGGCTGGGGCTATAACGTCGCGCTGGCGAACGGCGGGCGACAGGCGTTGGAGCAGGTGCGGGAACAGGTTTTTGATCTTGTGCTTTGCGATGTGCGAATGGCGGAGATGGACGGCATCGCCACGCTGAAAGAGATCaa                                           >  2:155472/1‑150 (MQ=255)                                                                                                        gCTTTACTGCGCGGCTGGGGCTATAACGTCGCGCTGGCGAACGGCGGGCGACAGGCGTTGGAGCAGGTGCGGGAACAGGTTTTTGATCTTGTGCTTTGCGATGTGCGAATGGCGGAGATGGACGGCATCGCCACGCTGAAAGAGATCaa                                           >  2:519837/1‑149 (MQ=255)                                                                                                               tGCGCGGCTGGGGCTATAACGTCGCGCTGGCGAACGGCGGGCGACAGGCGTTGGAGCAGGTGCGGGAACAGGTTTTTGATCTTGTGCTTTGCGATGTGCGAATGGCGGAGATGGACGGCATCGCCACGCTGAAAGAGATCAAAGCGTTaa                                   >  1:64269/1‑150 (MQ=255)                                                                                                                    ggCTGGGGCTATAACGTCGCGCTGGCGAACGGCGGGCGACAGGCGTTGGAGCAGGTGCGGGAACAGGTTTTTGATCTTGTGCTTTGCGATGTGCGAATGGCGGAGATGGACGGCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCgg                             >  2:729145/1‑151 (MQ=255)                                                                                                                      cTGGGGCTATAACGTCGCGCTGGCGAACGGCGGGCGACAGGCGTTGGAGCAGGTGCGGGAACAGGTTTTTGATCTTGTGCTTTGCGATGTGCGAATGGCGGAGATGGACGGCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGc                            >  2:667617/1‑150 (MQ=255)                                                                                                                        ggggCTATAACGTCGCGCTGGCGAGCGGCGGGCGACAGGCGTTGGAGCAGGTGCGGGAACAGGTTTTTGATCTTGTGCTTTGCGATGTGCGAATTGCGGAGATGGACGGCATCGCCACGCTGAAAGAGATCAAAGCGTTAACCCCGGCaa                          <  1:23857/150‑1 (MQ=255)                                                                                                                              ataACGTCGCGCTGGCGAACGGCGGGCGACAGGCGTTGGAGCAGGTGCGGGAACAGGTTTTTGATCTTGTGCTTTGCGATGTGCGAATGGCGGAGATGGACGGCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCgg                    >  2:72087/1‑150 (MQ=255)                                                                                                                              ataACGTCGCGCTGGCGAACGGCGGGCGACAGGCGTTGGAGCAGGTGCGGGAACAGGTTTTTGATCTTGTGCTTTGCGATGTGCGAATGGCGGAGATGGACGGCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCg                     <  1:324002/149‑1 (MQ=255)                                                                                                                                 aCGTCGCGCTGGCGAACGGCGGGCGACAGGCGTTGGAGCAGGTGCGGGAACAGGTTTTTGATCTTGTGCTTTGCGATGTGCGAATGGCGGAGATGGACGGCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCt                <  2:301859/151‑1 (MQ=255)                                                                                                                                  ctgcgggcTGGCGAGCGGCGGGCGGCAGGCGTTGGAGCAGGTGCGGGAACAGGTTTTTGATCTTGTGCTTTGCGATGTGCGAATGGCGGAGATGGACGGCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCt                <  2:255702/144‑1 (MQ=255)                                                                                                                                      gcgcTGGCGAACGGCGGGCGACAGGCGTTGGAGCAGGTGCGGGAACAGGTTTTTGATCTTGTGCTTTGCGATGTGCGAATGGCGGAGATGGACGGCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTa           <  2:724552/151‑1 (MQ=255)                                                                                                                                             cGAACGGCGGGCGACAGGCGTTGGAGCAGGTGCGGGAACAGGTTTTTGATCTTGTGCTTTGCGATGTGCGAATGGCGGAGATGGACGGCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGc    <  2:240548/151‑1 (MQ=255)                                                                                                                                               aaCGGCGGGCGACAGGCGTTGGAGCAGGTGCGGGAACAGGTTTTTGATCTTGTGCTTTGCGATGTGCGAATGGCGGAGATGGACGGCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGt  <  1:320131/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                    GGCTTCCGGTCAATATTACGCGTAAGGACCCACAAGGATGACGCACGATAATATCGATATTCTGGTGGTGGATGATGACATTAGCCACTGCACTATTTTGCAGGCTTTACTGCGCGGCTGGGGCTATAACGTCGCGCTGGCGAACAGCGGGCGACAGGCGTTGGAGCAGGTGCGGGAACAGGTTTTTGATCTTGTGCTTTGCGATGTGCGAATGGCGGAGATGGACGGCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTGCTGATTATGACTGCGT  >  NC_000913/4203283‑4203575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑