Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,286,249	T→C	T375A (ACC→GCC)	acs ←	acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,286,249	0	T	C	100.0%	179.3 / NA	52	T375A (ACC→GCC)	acs	acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/23);  total (29/23)

TGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTGGGTACGCCTTC  >  NC_000913/4286102‑4286381                                                                                                                                                    |                                                                                                                                     tGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGcc                                                                                                                                   >  1:56564/1‑151 (MQ=255)  gATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCt                                                                                                                                  >  1:22209/1‑151 (MQ=255)     cATGACACCACCGGTTTCGGTCTGCCACCAGGCATCGACCACCGCCCATTTCTAGTTGCCGCTTTTTTTCCAGTACCACTCCCACGCTTCCGGGATAATTGGCTCGCCCACGGACCAGGTAAGGCGCAGCGACGAACGGTCGGCGCCtt                                                                                                                                 >  1:712912/1‑149 (MQ=255)     cATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTc                                                                                                                                <  1:546490/150‑1 (MQ=255)      aTGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCg                                                                                                                               >  1:586877/1‑150 (MQ=255)         aaaCCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGAt                                                                                                                             <  1:632414/149‑1 (MQ=255)         aaaCCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATc                                                                                                                            <  1:314993/150‑1 (MQ=255)         aaaCCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCg                                                                                                                           >  2:277538/1‑151 (MQ=255)            ccgccgGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACACTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCtt                                                                                                                        >  1:336757/1‑151 (MQ=255)               ccgGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTAt                                                                                                                     >  1:229656/1‑151 (MQ=255)                cgGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATc                                                                                                                    <  1:151723/151‑1 (MQ=255)                 ggTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCg                                                                                                                   >  2:503993/1‑151 (MQ=255)                     tCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCtt                                                                                                               >  2:646984/1‑151 (MQ=255)                        gTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCg                                                                                                            <  1:585325/151‑1 (MQ=255)                            gCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGc                                                                                                           <  2:111558/148‑1 (MQ=255)                            gCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCa                                                                                                         <  2:743811/150‑1 (MQ=255)                             ccaccaGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATc                                                                                                       >  1:631374/1‑151 (MQ=255)                             ccaccaGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATc                                                                                                       >  1:689565/1‑151 (MQ=255)                                      aTCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGcgcgcg                                                                                               <  2:285272/150‑1 (MQ=255)                                        cGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGa                                                                                             <  2:56595/150‑1 (MQ=255)                                             accGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCg                                                                                       >  1:24777/1‑151 (MQ=255)                                              ccGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGt                                                                                      <  1:237006/151‑1 (MQ=255)                                              ccGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGt                                                                                      <  2:165548/151‑1 (MQ=255)                                                ggACATTTCCCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTgg                                                                                    >  2:493215/1‑151 (MQ=255)                                                 gACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTggg                                                                                   >  2:241395/1‑151 (MQ=255)                                                   cATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTg                                                                                 >  2:194539/1‑151 (MQ=255)                                                     tttCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCg                                                                               >  1:129177/1‑151 (MQ=255)                                                      ttCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCAGATCGCCGTGGGTGCgg                                                                              >  1:83644/1‑151 (MQ=255)                                                          cGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGtata                                                                          <  2:86658/151‑1 (MQ=255)                                                              gCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATag                                                                         >  2:116947/1‑148 (MQ=255)                                                                 gATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAat                                                                    >  2:574959/1‑150 (MQ=255)                                                                  aTTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAatat                                                                  >  2:310546/1‑151 (MQ=255)                                                                          ccAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTg                                                           <  2:214545/150‑1 (MQ=255)                                                                            aGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATg                                                        <  1:481373/151‑1 (MQ=255)                                                                                   cTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTcc                                                 >  1:233141/1‑151 (MQ=255)                                                                                          gCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCt                                          <  1:664051/151‑1 (MQ=255)                                                                                            ttCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTgc                                        >  2:684256/1‑151 (MQ=255)                                                                                             tCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTgc                                        <  2:359130/150‑1 (MQ=255)                                                                                               cGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGcc                                     >  1:506364/1‑151 (MQ=255)                                                                                                gggTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGcc                                     >  1:66903/1‑150 (MQ=255)                                                                                                         ggCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAg                           <  2:606207/151‑1 (MQ=255)                                                                                                         ggCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAg                           <  2:235006/151‑1 (MQ=255)                                                                                                               cccACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCg                     >  2:32692/1‑151 (MQ=255)                                                                                                                ccACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCgg                    >  1:91921/1‑151 (MQ=255)                                                                                                                 cACGGAACCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGc                   <  1:567751/151‑1 (MQ=255)                                                                                                                      aaCCGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGtt              <  2:720122/151‑1 (MQ=255)                                                                                                                       aCCGAGAATGCGCAGCGAAGAACGGTCGGCGCATTCGATCGCGTTAACGCCTTCCGCCATCAGCGCGCGGATCGTCGTGGGTGTGGAATAGAGACTATTGACTTGATGCGTGTCCACCACCTGCGCCATAAGGTCAGGCGTCGGCCCGtt              >  1:101799/1‑150 (MQ=255)                                                                                                                        ccGAGAATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTgg            <  2:691854/151‑1 (MQ=255)                                                                                                                          gagaATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTGGGt          <  2:258520/151‑1 (MQ=255)                                                                                                                           agaATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGACTATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTGGGt          >  1:335099/1‑150 (MQ=255)                                                                                                                           agaATGCGCAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTGGGTa         <  2:610464/151‑1 (MQ=255)                                                                                                                                  gcAGCGACGAACGGTCGGCGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTGGGTACGCCTTc  >  2:161142/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                     TGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTGGGTACGCCTTC  >  NC_000913/4286102‑4286381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑