Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,306,735	A→G	intergenic (‑138/+135)	yjcS ← / ← alsK	metallo‑beta‑lactamase superfamily protein/D‑allose kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,306,735	0	A	G	100.0%	171.2 / NA	48	intergenic (‑138/+135)	yjcS/alsK	metallo‑beta‑lactamase superfamily protein/D‑allose kinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/23);  total (25/23)

AGTTATTCATATAAATCTCCATTTATCATTTATGTCGTAAATATGTAATCCATGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCATTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGG  >  NC_000913/4306588‑4306877                                                                                                                                                    |                                                                                                                                               agTTATTCATATAAATCTCCATTTATCATTTATGTCGTAAATATGTAATCCATGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGttt                                                                                                                                             >  1:695080/1‑151 (MQ=255)   ttATTCATATAAATCTCCATTTATCATTTATGTCGTAAATATGTAATCCATGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTtg                                                                                                                                            >  2:385144/1‑150 (MQ=255)    tATTCATATAAATCTCCATTTATCATTTATGTCGTAAATATGTAATCCATGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTtgtg                                                                                                                                          >  1:506794/1‑151 (MQ=255)      ttCATATAAATCTCCATTTATCATTTATGTCGTAAATATGTAATCCATGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCt                                                                                                                                        >  1:726414/1‑151 (MQ=255)        cATATAAATCTCCATTTATCATTTATGTCGTAAATATGTAATCCATGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTg                                                                                                                                       >  2:586940/1‑150 (MQ=255)              aaTCTCCATTTATCATTTATGTCGTAAATATGTAATCCATGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAggg                                                                                                                                <  1:596114/151‑1 (MQ=255)                  tcCATTTATCATTTATGTCGTAAATATGTAATCCATGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGaaa                                                                                                                             >  2:489140/1‑150 (MQ=255)                   ccATTTATCATTTATGTCGTAAATATGTAATCCATGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAAt                                                                                                                           >  1:94373/1‑151 (MQ=255)                        tatcatttatGTCGTAAATATGTAATCCATGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACa                                                                                                                       <  2:19831/150‑1 (MQ=255)                           catttatGTCGTAAATATGTAATCCATGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTaa                                                                                                                   <  2:355169/151‑1 (MQ=255)                                atGTCGTAAATATGTAATCCATGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACt                                                                                                               <  2:132472/150‑1 (MQ=255)                                 tGTCGTAAATATGTAATCCATGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTAt                                                                                                             >  2:376983/1‑151 (MQ=255)                                  gTCGTAAATATGTAATCCATGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTAt                                                                                                             <  2:489617/150‑1 (MQ=255)                                     gTAAATATGTAATCCATGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCAtt                                                                                                         <  2:505031/151‑1 (MQ=255)                                     gTAAATATGTAATCCATGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCAtt                                                                                                         <  2:91275/151‑1 (MQ=255)                                       aaaTATGTAATCCATGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGc                                                                                                       >  2:23616/1‑151 (MQ=255)                                       aaaTATGTAATCCATGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGc                                                                                                       <  2:445138/151‑1 (MQ=255)                                       aaaTATGTAATCCATGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGc                                                                                                       >  2:121471/1‑151 (MQ=255)                                             gTAATCCATGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGaaaa                                                                                                 <  2:742951/151‑1 (MQ=255)                                                   cATGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAaataat                                                                                             <  1:589278/149‑1 (MQ=255)                                                    aTGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATg                                                                                          >  2:424297/1‑151 (MQ=255)                                                      gCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAg                                                                                        <  2:744235/151‑1 (MQ=255)                                                          aaTACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGt                                                                                    <  2:411911/151‑1 (MQ=255)                                                           aTACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGtt                                                                                   >  2:40752/1‑151 (MQ=255)                                                             aCGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGtttt                                                                                 <  1:13559/151‑1 (MQ=255)                                                             aCGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGtttt                                                                                 <  1:557623/151‑1 (MQ=255)                                                                 cATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTAtgt                                                                             >  1:444993/1‑151 (MQ=255)                                                                 cATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTAtg                                                                              <  2:254858/150‑1 (MQ=255)                                                                 cATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTAtg                                                                              <  1:424246/150‑1 (MQ=255)                                                                  aTTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTAtgtg                                                                            >  2:152852/1‑151 (MQ=255)                                                                   tttAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATgtgt                                                                           >  1:557316/1‑151 (MQ=255)                                                                   tttAAAGCTGGCGGTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGAAAGTGCTGAAGAGGGAAAATTACAGTAAAACTATCACTGCAGAAAATACTATGAGTCGATTTAt                                                                               <  1:311994/147‑1 (MQ=255)                                                                    ttAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGtt                                                                          >  2:239418/1‑151 (MQ=255)                                                                      aaaGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGttt                                                                         <  2:301624/150‑1 (MQ=255)                                                                         gCTGGCGTTATCATAATTGGGAAATCGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTttatta                                                                     <  1:384814/151‑1 (MQ=255)                                                                             gCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATtatat                                                                  >  1:590971/1‑150 (MQ=255)                                                                              cGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATtatatat                                                                >  2:361490/1‑151 (MQ=255)                                                                                     aTAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTAc                                                         >  2:404170/1‑151 (MQ=255)                                                                                           gggTAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGt                                                     >  1:513977/1‑149 (MQ=255)                                                                                           gggAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCg                                                   >  1:164926/1‑151 (MQ=255)                                                                                             gAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGtt                                                 >  1:469065/1‑151 (MQ=255)                                                                                                   gCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGcac                                            <  2:164959/150‑1 (MQ=255)                                                                                                     gACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGc                                         >  2:209131/1‑151 (MQ=255)                                                                                                           aataaGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACa                                   <  2:237556/151‑1 (MQ=255)                                                                                                              aaGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGCAAATGGACAATTACAGTAAAACTATCCTTGCAGAAAATAATATGACTCGTTTTATGTGTCTATTCTCTATATTGGACCTGTCGTTGGCACAGAGGGACATTg                                >  1:693572/1‑151 (MQ=255)                                                                                                                                    cgcCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTAc          >  2:93032/1‑151 (MQ=255)                                                                                                                                      ccATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTc        <  1:152819/151‑1 (MQ=255)                                                                                                                                             attaGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATgggg  <  2:618847/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                               AGTTATTCATATAAATCTCCATTTATCATTTATGTCGTAAATATGTAATCCATGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCATTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGG  >  NC_000913/4306588‑4306877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑