Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,403,176	C→T	P380L (CCG→CTG)	hflK →	modulator for HflB protease specific for phage lambda cII repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,403,176	0	C	T	100.0%	132.8 / NA	35	P380L (CCG→CTG)	hflK	modulator for HflB protease specific for phage lambda cII repressor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/13);  total (22/13)

ATGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAGCGTCAGGGGGAATAACGATGCGTAAGTCAGTTATCGCG  >  NC_000913/4403028‑4403320                                                                                                                                                     |                                                                                                                                                 aTGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCtgctgc                                                                                                                                                >  2:139884/1‑151 (MQ=255)   ggAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCa                                                                                                                                              <  1:707751/151‑1 (MQ=255)      aaaaGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGcc                                                                                                                                           >  2:456065/1‑151 (MQ=255)       aaaGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCct                                                                                                                                          >  2:503819/1‑151 (MQ=255)                 gTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCcac                                                                                                                                <  2:408127/151‑1 (MQ=255)                 gTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCcac                                                                                                                                <  1:612481/151‑1 (MQ=255)                             aaGTCCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCTTTAGACCAGATGCTGAAAGGTGGTAATGCTCCTGCGGCGAAGAGCGATAACGTTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGagc                                                                                                                    <  1:509220/151‑1 (MQ=255)                               gTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGagcaa                                                                                                                  <  1:215484/151‑1 (MQ=255)                               gTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGagca                                                                                                                   >  1:721961/1‑150 (MQ=255)                                      ttAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAacac                                                                                                           >  2:404906/1‑151 (MQ=255)                                              aaaGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTcc                                                                                                    <  2:769622/150‑1 (MQ=255)                                                    ggCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTc                                                                                             <  2:208611/151‑1 (MQ=255)                                                            gATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGat                                                                                     <  1:278386/151‑1 (MQ=255)                                                              tGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGatat                                                                                   >  1:555648/1‑151 (MQ=255)                                                               ggTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGAtatt                                                                                  >  1:279751/1‑151 (MQ=255)                                                                 ttCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGAtattat                                                                                >  2:122554/1‑151 (MQ=255)                                                                  tCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGAtattat                                                                                <  2:102557/150‑1 (MQ=255)                                                                              ccAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAAcgccgc                                                                   >  1:143534/1‑151 (MQ=255)                                                                                          aGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAg                                                       <  1:415323/151‑1 (MQ=255)                                                                                            gtggtAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGc                                                      >  1:746282/1‑150 (MQ=255)                                                                                            gtggtAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCg                                                     >  1:636122/1‑151 (MQ=255)                                                                                                 aaCGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACg                                                >  1:359346/1‑151 (MQ=255)                                                                                                   cGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGAc                                              >  2:367618/1‑151 (MQ=255)                                                                                                    gCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACt                                             >  2:614327/1‑151 (MQ=255)                                                                                                      cccTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTAc                                           <  1:143041/151‑1 (MQ=255)                                                                                                         tGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTAcc                                          >  1:300702/1‑149 (MQ=255)                                                                                                            ggcgAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAGCGt                                     >  1:26975/1‑151 (MQ=255)                                                                                                                     cGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAGCGTCAGGGGGa                             >  2:450726/1‑150 (MQ=255)                                                                                                                         aaCGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAGCGTCAGGGGGAATAAc                        >  2:557665/1‑151 (MQ=255)                                                                                                                               gCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAGCGTCAGGGGGAATAACGATGCg                  >  2:627458/1‑151 (MQ=255)                                                                                                                                       tCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAGCGTCAGGGGGAATAACGATGCGTAAGTc            >  2:178466/1‑149 (MQ=255)                                                                                                                                          gctgcGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAGCGTCAGGGGGAATAACGATGCGTAAGTCAGtt        >  1:542995/1‑150 (MQ=255)                                                                                                                                             gcGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAGCGTCAGGGGGAATAACGATGCGTAAGTCAGTTATcg    <  1:467550/151‑1 (MQ=255)                                                                                                                                             gcGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAGCGTCAGGGGGAATAACGATGCGTAAGTCAGTTATc     <  2:402229/150‑1 (MQ=255)                                                                                                                                               gTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAGCGTCAGGGGGAATAACGATGCGTAAGTCAGTTATcgcg  >  1:601138/1‑151 (MQ=255)                                                                                                                                               gTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAGCGTCAGGGGGAATAACGATGCGTAAGTCAGTTATcg    >  1:627081/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                 ATGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAGCGTCAGGGGGAATAACGATGCGTAAGTCAGTTATCGCG  >  NC_000913/4403028‑4403320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑