Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,550,166	A→G	T377T (ACT→ACC)	gntP ←	fructuronate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,550,166	0	A	G	100.0%	165.0 / NA	46	T377T (ACT→ACC)	gntP	fructuronate transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/22);  total (24/22)

CCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATG  >  NC_000913/4550020‑4550310                                                                                                                                                   |                                                                                                                                                 ccccAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTg                                                                                                                                              <  2:286000/151‑1 (MQ=255)      ggTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGa                                                                                                                                         <  1:144521/151‑1 (MQ=255)         tttCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCt                                                                                                                                      >  1:625895/1‑151 (MQ=255)           tCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGAACAACCAGAAAGATGCATCATTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTa                                                                                                                                     <  2:57261/150‑1 (MQ=255)               cGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGAtt                                                                                                                                <  2:756170/151‑1 (MQ=255)               cGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGAtt                                                                                                                                <  2:644196/151‑1 (MQ=255)                      tttACTGACAGGTCAAAGTAACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTgcagca                                                                                                                          <  2:428795/150‑1 (MQ=255)                              cAGGTCAAAGTAACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATc                                                                                                                 >  1:329748/1‑151 (MQ=255)                                  tCAAAGTAACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCcgg                                                                                                             >  1:506417/1‑151 (MQ=255)                                   cAAAGTAACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCcggc                                                                                                            >  1:127897/1‑151 (MQ=255)                                   cAAAGTAACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCcggc                                                                                                            >  2:334435/1‑151 (MQ=255)                                        tAACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCcggcggcg                                                                                                        >  1:735473/1‑150 (MQ=255)                                           ccTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCa                                                                                                    <  2:637070/151‑1 (MQ=255)                                            cTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCAt                                                                                                   >  2:547458/1‑151 (MQ=255)                                              ttGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCg                                                                                                 >  2:132834/1‑151 (MQ=255)                                                gAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGcc                                                                                               <  1:107552/151‑1 (MQ=255)                                                  acaacCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCg                                                                                              >  1:468792/1‑150 (MQ=255)                                                     acCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAAc                                                                                          <  2:626753/151‑1 (MQ=255)                                                      ccAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAAc                                                                                          <  1:727231/150‑1 (MQ=255)                                                          aacgATGGATCGTTAATGTGGGGGAGGGTGTTGGAACCCGCAGCCGTCGTCAGTACCAGGAGCGCGGGCTTCGCGCCCAGCAGCTGACCGGTTGCTGGATCTAGGATTTCAGCACTGATAATCCCGTCGGCGGTCATCGccttgccg                                                                                         >  1:179382/4‑141 (MQ=255)                                                             gATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCt                                                                                  >  2:363846/1‑151 (MQ=255)                                                              aTGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTg                                                                                 >  2:94203/1‑151 (MQ=255)                                                                 cATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGAcc                                                                              >  1:127957/1‑151 (MQ=255)                                                                            tgGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGAc                                                                   >  1:29256/1‑151 (MQ=255)                                                                              ggTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACg                                                                  >  2:325666/1‑150 (MQ=255)                                                                              ggTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACg                                                                  >  1:611806/1‑150 (MQ=255)                                                                              ggTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACg                                                                  >  2:441756/1‑150 (MQ=255)                                                                                       gtTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGcac                                                         >  1:272918/1‑150 (MQ=255)                                                                                           gAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGa                                                    <  2:87944/151‑1 (MQ=255)                                                                                           gAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGa                                                    <  1:334402/151‑1 (MQ=255)                                                                                             aCCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATc                                                  >  2:607676/1‑151 (MQ=255)                                                                                                 gCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAgcc                                              <  1:718831/151‑1 (MQ=255)                                                                                                    gCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAgccatg                                           <  1:694455/151‑1 (MQ=255)                                                                                                       gTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAgccatgcca                                        <  1:678142/151‑1 (MQ=255)                                                                                                       gTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGCCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAgccatgcca                                        <  1:282885/151‑1 (MQ=255)                                                                                                                aCCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAgg                               <  1:743403/151‑1 (MQ=255)                                                                                                                      agcGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCg                            >  2:144203/1‑148 (MQ=255)                                                                                                                       gcgcCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCg                            >  2:603108/1‑147 (MQ=255)                                                                                                                          ccGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATAtt                     <  1:338967/151‑1 (MQ=255)                                                                                                                          ccGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATAtt                     <  2:506469/151‑1 (MQ=255)                                                                                                                            ggATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGc                   >  2:448255/1‑151 (MQ=255)                                                                                                                                  aCGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTggg             >  2:487385/1‑151 (MQ=255)                                                                                                                                  aCGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTggg             <  1:581154/151‑1 (MQ=255)                                                                                                                                          cAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAAc     >  1:762617/1‑151 (MQ=255)                                                                                                                                           aGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACa    <  1:484805/151‑1 (MQ=255)                                                                                                                                               gACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATg  <  1:737259/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                 CCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATG  >  NC_000913/4550020‑4550310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑