Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,559,131	C→T	M132I (ATG→ATA)	iadA ←	isoaspartyl dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,559,131	0	C	T	100.0%	170.7 / NA	44	M132I (ATG→ATA)	iadA	isoaspartyl dipeptidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/18);  total (26/18)

CATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCGG  >  NC_000913/4558984‑4559274                                                                                                                                                    |                                                                                                                                                cATATTGGCGAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATc                                                                                                                                              <  1:455967/151‑1 (MQ=255)    atTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCa                                                                                                                                            <  2:308204/150‑1 (MQ=255)             gTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATg                                                                                                                                  >  1:747177/1‑151 (MQ=255)                aTAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGGCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGcc                                                                                                                                <  1:126026/150‑1 (MQ=255)                   aaCGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCcttct                                                                                                                            <  2:142481/151‑1 (MQ=255)                        ccGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCAtt                                                                                                                       <  2:91877/151‑1 (MQ=255)                                    aaCGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATc                                                                                                                                              >  1:483021/1‑116 (MQ=255)                                    aaCGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATc                                                                                                                                              <  2:483073/116‑1 (MQ=255)                                            tCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCg                                                                                                    >  1:169296/1‑150 (MQ=255)                                                agaTGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAg                                                                                               <  1:653298/151‑1 (MQ=255)                                                 gaTGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAgg                                                                                              >  2:648703/1‑151 (MQ=255)                                                    ggCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGAt                                                                                           >  2:266901/1‑151 (MQ=255)                                                            tCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTg                                                                                   >  1:319541/1‑151 (MQ=255)                                                             cACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTgg                                                                                  >  1:219598/1‑151 (MQ=255)                                                              aCGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGc                                                                                 >  1:574409/1‑151 (MQ=255)                                                              aCGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGc                                                                                 >  2:636789/1‑151 (MQ=255)                                                                      cacaCGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGAt                                                                          <  1:88571/150‑1 (MQ=255)                                                                      cacaCGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATa                                                                         <  2:238794/151‑1 (MQ=255)                                                                       acacGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGAt                                                                          >  2:420625/1‑149 (MQ=255)                                                                        cacGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGAt                                                                          >  1:192635/1‑148 (MQ=255)                                                                             tcgatAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTc                                                                    <  2:88448/149‑1 (MQ=255)                                                                                          cGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGacc                                                     <  2:393525/151‑1 (MQ=255)                                                                                               ttttCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGaccaacc                                                 >  1:658531/1‑150 (MQ=255)                                                                                                tttCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGaccaacca                                                <  2:362062/150‑1 (MQ=255)                                                                                                tttCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGaccaacca                                                <  1:136929/150‑1 (MQ=255)                                                                                                  tCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACtg                                             >  1:412843/1‑151 (MQ=255)                                                                                                      cGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACtgacg                                          >  2:110107/1‑150 (MQ=255)                                                                                                       ggAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACtgacgtg                                        >  1:412953/1‑151 (MQ=255)                                                                                                        gAACCCGTAACGGGGCGGGGAGGGACATGAAAAGCTCCGGACAGTATCCAGGCGCTTATGGCTTCTTCATTGAGCGCTCGGGTCTAGGCGAGCAGGGATTGCGGGTGGAGAGAGATAGAGTCGGAGCCCAGCAGACCAACCACtgacgt                                         >  1:108511/1‑149 (MQ=255)                                                                                                         aaCCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACtgacgtgac                                      >  1:96442/1‑151 (MQ=255)                                                                                                             cGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGccc                                  >  1:84062/1‑151 (MQ=255)                                                                                                               tAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGc                                >  1:371003/1‑151 (MQ=255)                                                                                                                 aTGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGCtt                              <  1:396703/151‑1 (MQ=255)                                                                                                                  tGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGCTTc                             >  1:374116/1‑151 (MQ=255)                                                                                                                  tGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGCTTc                             >  1:66363/1‑151 (MQ=255)                                                                                                                     tGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGCTTCCGt                          >  1:261160/1‑151 (MQ=255)                                                                                                                       cGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGCTTCCGTCa                        <  2:371050/151‑1 (MQ=255)                                                                                                                           aaGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGCTTCCGTCAGGCg                    <  2:139307/151‑1 (MQ=255)                                                                                                                            aGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGCTTCCGTCAGGCg                    >  1:510316/1‑150 (MQ=255)                                                                                                                               gACATGATAAGCGCCGGTCAGTGTCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGCTTCCGTCAGGCGACtt                <  2:218218/151‑1 (MQ=255)                                                                                                                                 cATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGCTTCCGTCAGGCGACtt                >  2:379919/1‑149 (MQ=255)                                                                                                                                  aTGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGCTTCCGTCAGGCGACTTAgc             >  2:293485/1‑151 (MQ=255)                                                                                                                                             cGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCgg  <  1:544042/151‑1 (MQ=255)                                                                                                                                             cGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCgg  >  2:236024/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                CATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCGG  >  NC_000913/4558984‑4559274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑