Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,608,413	C→T	R77C (CGT→TGT)	rimI →	ribosomal‑protein‑S18‑alanine N‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,608,413	0	C	T	100.0%	181.4 / NA	48	R77C (CGT→TGT)	rimI	ribosomal‑protein‑S18‑alanine N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/19);  total (29/19)

ACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGCGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTC  >  NC_000913/4608275‑4608560                                                                                                                                           |                                                                                                                                                    aCGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTgg                                                                                                                                         <  1:647555/151‑1 (MQ=255)    tttGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGa                                                                                                                                       >  2:739997/1‑150 (MQ=255)               ccAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGctgctg                                                                                                                           >  2:127452/1‑151 (MQ=255)                 aGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTgg                                                                                                                          >  1:292815/1‑150 (MQ=255)                 aGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTgg                                                                                                                          >  2:442725/1‑150 (MQ=255)                    gCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACa                                                                                                                      <  2:733176/151‑1 (MQ=255)                      gAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATc                                                                                                                    >  2:764830/1‑151 (MQ=255)                      gAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATc                                                                                                                    >  2:759822/1‑151 (MQ=255)                                 cAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATcgacg                                                                                                           <  1:215454/149‑1 (MQ=255)                                 cAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATcga                                                                                                             <  2:631361/147‑1 (MQ=255)                                    cTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTg                                                                                                      >  2:360381/1‑151 (MQ=255)                                      ttCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTgg                                                                                                     >  2:566764/1‑150 (MQ=255)                                        cAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGaa                                                                                                   >  1:17128/1‑150 (MQ=255)                                          gTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGaaaa                                                                                                 >  2:643804/1‑150 (MQ=255)                                            tAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAAcg                                                                                              >  2:362686/1‑151 (MQ=255)                                            tAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAAc                                                                                               <  1:564914/150‑1 (MQ=255)                                            tAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAAc                                                                                               <  1:397951/150‑1 (MQ=255)                                             aaCGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAAcgc                                                                                             >  1:442117/1‑151 (MQ=255)                                             aaCGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAAcg                                                                                              >  2:534664/1‑150 (MQ=255)                                             aaCGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAAcg                                                                                              <  2:268314/150‑1 (MQ=255)                                                  aaaaCGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGt                                                                                        >  1:422100/1‑151 (MQ=255)                                                   aaaCGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTg                                                                                       >  2:754657/1‑151 (MQ=255)                                                    aaCGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTgg                                                                                      >  2:183201/1‑151 (MQ=255)                                                      cGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCg                                                                                    >  2:649379/1‑151 (MQ=255)                                                               ggcggcGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATg                                                                            <  1:702692/150‑1 (MQ=255)                                                                gcggcgTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGc                                                                          <  1:534612/151‑1 (MQ=255)                                                                 cggcgTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCt                                                                         >  2:169224/1‑151 (MQ=255)                                                                    cgTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTgg                                                                       >  1:6374/1‑150 (MQ=255)                                                                       ttGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGt                                                                   >  1:56815/1‑151 (MQ=255)                                                                           gATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGt                                                               <  2:564433/151‑1 (MQ=255)                                                                               aCGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCtt                                                           <  2:586160/151‑1 (MQ=255)                                                                                 gCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCa                                                         <  2:558294/151‑1 (MQ=255)                                                                                  cAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCa                                                         >  1:544578/1‑150 (MQ=255)                                                                                    aGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAAc                                                      >  1:668104/1‑151 (MQ=255)                                                                                        gtgCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTg                                                  <  2:493134/151‑1 (MQ=255)                                                                                         tgCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTgc                                                 >  1:523253/1‑151 (MQ=255)                                                                                             ggATGACGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTgccgcc                                             >  2:270169/1‑151 (MQ=255)                                                                                             ggATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTgccgcc                                             <  1:211214/151‑1 (MQ=255)                                                                                                    gCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGccc                                      >  2:147853/1‑151 (MQ=255)                                                                                                                 atatTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGttt                         >  2:522673/1‑151 (MQ=255)                                                                                                                 atatTGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGttt                         <  2:358552/151‑1 (MQ=255)                                                                                                                     tGCGGTCGATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGc                     >  1:234484/1‑151 (MQ=255)                                                                                                                            gATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACg              >  2:614988/1‑151 (MQ=255)                                                                                                                            gATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACg              <  1:320397/151‑1 (MQ=255)                                                                                                                            gATCCTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCAGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGTCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAAc               >  1:519345/1‑150 (MQ=255)                                                                                                                               ccTGACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAg            <  2:538661/150‑1 (MQ=255)                                                                                                                                  gACTATCAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGcg         <  2:435999/150‑1 (MQ=255)                                                                                                                                        cAGTGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTc  <  2:563138/151‑1 (MQ=255)                                                                                                                                           |                                                                                                                                                    ACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGATCCTGACTATCAGCGTCAGGGATTGGGAAGGGCGCTGCTGGAACATCTGATCGACGAACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTC  >  NC_000913/4608275‑4608560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑