Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,617,397 C→T T25T (ACC→ACT)  deoC → 2‑deoxyribose‑5‑phosphate aldolase, NAD(P)‑linked

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,617,3970CT93.2% 198.6 / ‑7.1 59T25T (ACC→ACT) deoC2‑deoxyribose‑5‑phosphate aldolase, NAD(P)‑linked
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (29/26);  minor base G (0/4);  total (29/30)
Fisher's exact test for biased strand distribution p-value = 1.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTA  >  NC_000913/4617252‑4617536
                                                                                                                                                 |                                                                                                                                           
tGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACtgacg                                                                                                                                         <  2:291502/150‑1 (MQ=255)
  cGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACtgacg                                                                                                                                         >  2:429324/1‑148 (MQ=255)
      gTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACTGACGAGAAAGt                                                                                                                                  >  2:770084/1‑151 (MQ=255)
       tAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACTGACGAGAAAGt                                                                                                                                  <  2:521220/150‑1 (MQ=255)
           tGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACTGACGAGAAAGTGATCg                                                                                                                             >  2:737726/1‑151 (MQ=255)
           tGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACTGACGAGAAAGTGATCg                                                                                                                             >  1:505415/1‑151 (MQ=255)
               aGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACTGACGAGAAAGTGATCGCCCt                                                                                                                         >  1:148459/1‑151 (MQ=255)
               aGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACTGACGAGAAAGTGATCGCCCt                                                                                                                         <  2:236303/151‑1 (MQ=255)
                     cTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACTGACGAGAAAGTGATCGCCCTGTGtc                                                                                                                    >  2:97531/1‑150 (MQ=255)
                     cTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACTGACGAGAAAGTGATCGCCCTGTGtc                                                                                                                    >  1:191448/1‑150 (MQ=255)
                       aaCAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACTGACGAGAAAGTGATCGCCCTGTGtca                                                                                                                   <  2:548635/149‑1 (MQ=255)
                          aaaCTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACTGACGAGAAAGTGATCGCCCTGTGTCATCAgt                                                                                                              <  1:482326/151‑2 (MQ=255)
                          aaaCTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACTGACGAGAAAGTGATCGCCCTGTGTCATCAgg                                                                                                              <  2:647550/151‑1 (MQ=255)
                           aaCTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACTGACGAGAAAGTGATCGCCCTGTGTCATCAGGc                                                                                                             >  2:133460/1‑151 (MQ=255)
                               cGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACTGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCaaa                                                                                                         <  2:688057/151‑1 (MQ=255)
                                 cAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACTGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAAc                                                                                                       <  1:507943/151‑1 (MQ=255)
                                  aaGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACTGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACt                                                                                                      <  2:606355/151‑1 (MQ=255)
                                   aGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACTGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTc                                                                                                     <  2:626084/151‑1 (MQ=255)
                                         aTTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACTGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCg                                                                                               <  1:298818/151‑1 (MQ=255)
                                             tATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACTGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCa                                                                                            <  2:666231/150‑1 (MQ=255)
                                              aTTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACTGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAAt                                                                                          <  2:721223/151‑1 (MQ=255)
                                                       aaGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACTGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCt                                                                                 >  1:4714/1‑151 (MQ=255)
                                                            aGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACTGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTg                                                                            >  1:154598/1‑151 (MQ=255)
                                                            aGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACTGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTg                                                                            >  2:689366/1‑151 (MQ=255)
                                                            aGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACTGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTg                                                                            >  2:72700/1‑151 (MQ=255)
                                                             ggAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACTGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGt                                                                           >  1:355386/1‑151 (MQ=255)
                                                               agaATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACTGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGtat                                                                         >  2:57815/1‑151 (MQ=255)
                                                                 aatgaaatgaCTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACTGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGtatct                                                                       >  2:646708/1‑151 (MQ=255)
                                                                 aatgaaatgaCTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACTGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGtatc                                                                        >  1:755392/1‑150 (MQ=255)
                                                                   tgaaatgaCTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACTGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGtatctat                                                                     >  2:391122/1‑151 (MQ=255)
                                                                       atgaCTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGCCCTGACCCCCCTGAATGACGACGACACTGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTc                                                                 <  2:50801/151‑1 (MQ=255)
                                                                         gcttGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGCCCTGACCCCCCTGAATGACGACGACACTGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAACCTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCg                                                                <  2:605098/147‑1 (MQ=255)
                                                                          aCTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGCCCTGACCCCCCTGAATGACGACGACACTGACGAAAAAGTGATCGCCCTGTGTCATCAGGCAAAACCCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCt                                                              <  1:176560/151‑1 (MQ=255)
                                                                             gATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACTGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCttt                                                            >  2:632425/1‑150 (MQ=255)
                                                                              aTCTGAAAGCAAGCAGCCTGCGTGCACGGAAATGGGTGAACCTGACCCCCCTGAAGGACGACGACACTGCAGAGAAAGTGTTCGCATTGTTTCACCAGCCAAAAACTCAGGGCGGAAATACCGCCGCTATCTGTATCTATCCTCGCTTTa                                                           <  1:369596/150‑1 (MQ=255)
                                                                                 tGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACTGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATccc                                                       >  2:15321/1‑151 (MQ=255)
                                                                                  gAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACTGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCg                                                      >  2:216658/1‑151 (MQ=255)
                                                                                   aaaGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACTGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCg                                                      >  1:478801/1‑150 (MQ=255)
                                                                                   aaaGCAAGCAGCCTGCGTGCACGGAAATTGATGGACCTGACCCCCCTGAATGACGACGACACTGACGAGAAAGTGATCGCCCTGTGTCATCAGCCCAAAACTCCGGTCGGAAATACCGCCGCTATCTGTATCTATCCTCGCTTTAt                                                          <  1:20838/146‑1 (MQ=255)
                                                                                   aaaGCAAGCAGCCGGCGTGCACTGAAATTGAGGGCCCTGACCCCCCTGAAGGCCGACGACACTGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGAAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGa                                                     <  1:561690/151‑1 (MQ=255)
                                                                                     agcaagcaGCCTGCGTGCACTAAAATTGATGGCCCTGACCCCCCTGAATGACGACGACACTGACGAGAAAGTGACCGCCCTGTGTCATCAGCCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGAtt                                                   <  2:747996/151‑1 (MQ=255)
                                                                                        aagcaGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACTGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCt                                                >  1:230057/1‑151 (MQ=255)
                                                                                            agcCTGCGTGCACTGAAATTGATGGCCCTGCCCCCCCTAAATGACGACGACACTGACGAGAAAGTGATCCCCCTGTGTCATCAGGCCAAAACTCCGTTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTc                                               <  2:263094/148‑1 (MQ=255)
                                                                                            agcCTGCGTGCACTGAAATTGATGGCCCTGACCCCCCTAAATGACGACGCCCCGGCCAAAAAATTGATCGCCCTTTGTCATCAGCCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGAtt                                                   <  1:269994/144‑1 (MQ=255)
                                                                                             gcCTGCGTGCACTGAAATTGATGGCCCTGACCCCCCTGAATGACGACGACACTGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTc                                               <  2:50178/147‑1 (MQ=255)
                                                                                              ccTGCGTGCACTGAAATTGATGGACCTGCCCACCCTGAATGACGACGACACTGACGAGAAAGTGATCGCCCTGTGTCATCAGGCAAAACCTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCaaa                                          <  2:769176/151‑1 (MQ=255)
                                                                                                tGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACTGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAAc                                        >  2:685515/1‑151 (MQ=255)
                                                                                                           aaaTTGTTGGACCGAACCCCCCTGAAGGACGAGGACACGGACGAGAAGGTGGCCCCCCTGTGTCAGCGGCCAAAAACTCCGGTGGGCAATACCGCCGCTATCTGTATCTATCCGCGCTTTATCCCGATTGCTCGCAAAACTCTg                                    <  1:540710/144‑1 (MQ=255)
                                                                                                           aaaTTGATGGACCTGACCACCCTGAATGACGACGACACTGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGc                             >  1:157089/1‑151 (MQ=255)
                                                                                                            aaTTGATGGACCTGACCACCCTGAATGACGACGACACTGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAaga                               >  1:272811/1‑148 (MQ=255)
                                                                                                            aaTTGATGGACCTGACCACCCTGAATGACGACGACACTGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACAGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGc                             >  1:180792/1‑150 (MQ=255)
                                                                                                              ttGATGGACCTGACCACCCTGAATGACGACGACACTGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAgg                          >  1:317068/1‑151 (MQ=255)
                                                                                                                  tGGCCCTGACCACCCTGAAGGACGACGACCCTGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAACCTCCGGTCGGCATTACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAg                           <  2:148574/146‑1 (MQ=255)
                                                                                                                       cTGACCACCCTGAATGACGACGACACTGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCgg                 >  2:360996/1‑151 (MQ=255)
                                                                                                                       cTGACCACCCTGAATGACGACGACACTGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCgg                 >  1:660935/1‑151 (MQ=255)
                                                                                                                          accaccCTGAATGACGACGACACTGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGaa               >  1:299269/1‑150 (MQ=255)
                                                                                                                            cccccTGAATGGCGACGACCCTTACTAGAAATGAATCCCTCTGTGTCATCAGTCCAAAGCGCCGTCCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATc            <  1:772773/149‑1 (MQ=255)
                                                                                                                            cccccTGAATGACGACGACCCGGACGAGAAAGTGATCGCCCTGTGTCATCAGCCAAAACCCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATc            <  1:633692/149‑1 (MQ=255)
                                                                                                                              cccTGAATGACGACGACACTGACGAGAAAGTGATCGCCCTGTGTCATCAGGCAAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATcc           <  2:529731/150‑1 (MQ=255)
                                                                                                                               ccTGAATGACGCCGACACGGACGAGAAAGTGATCGCCCGGTGTCATCAGCCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATcc           <  1:489691/149‑1 (MQ=255)
                                                                                                                                        cgacgacACTGACGAGAAAGTGATCGCCCTGTGTCATCAGGCAAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTa  <  2:299302/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                           
TGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTA  >  NC_000913/4617252‑4617536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑