Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	514,375	G→A	G128D (GGC→GAC)	cueR →	copper‑responsive regulon transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	514,375	0	G	A	100.0%	177.6 / NA	47	G128D (GGC→GAC)	cueR	copper‑responsive regulon transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/24);  total (23/24)

ACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATAT  >  NC_000913/514228‑514522                                                                                                                                                    |                                                                                                                                                    aCGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGactg                                                                                                                                                  <  1:428529/151‑1 (MQ=255)      aaaCGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGTGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGtc                                                                                                                                             <  2:641600/151‑1 (MQ=255)      aaaCGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGtc                                                                                                                                             >  2:301898/1‑151 (MQ=255)       aaCGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGtca                                                                                                                                            <  1:42802/151‑1 (MQ=255)         cGGCGCACGCTGGAGAAGGGGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGtcatc                                                                                                                                          >  1:360370/1‑151 (MQ=255)          ggCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGtcatca                                                                                                                                         <  2:458196/151‑1 (MQ=255)          ggCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGtcatca                                                                                                                                         <  1:180925/151‑1 (MQ=255)                 gCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCa                                                                                                                                  <  2:358130/151‑1 (MQ=255)                            tGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTa                                                                                                                         >  2:175466/1‑149 (MQ=255)                             ggCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTa                                                                                                                         >  2:652009/1‑148 (MQ=255)                                ggAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAagacg                                                                                                                    >  2:185557/1‑150 (MQ=255)                                    aTCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAagacgagacg                                                                                                               >  1:591968/1‑151 (MQ=255)                                        aaCGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTc                                                                                                           >  2:288368/1‑151 (MQ=255)                                         aCGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCg                                                                                                          >  1:335793/1‑151 (MQ=255)                                         aCGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCg                                                                                                          >  1:142580/1‑151 (MQ=255)                                            acacATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGat                                                                                                       >  2:761252/1‑151 (MQ=255)                                             cacaTTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGata                                                                                                      <  1:504944/151‑1 (MQ=255)                                                 ttGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGt                                                                                                   >  1:290083/1‑150 (MQ=255)                                                    aggagCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAggg                                                                                               <  1:719803/151‑1 (MQ=255)                                                        gCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTAt                                                                                           <  1:467564/151‑1 (MQ=255)                                                        gCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTAt                                                                                           <  1:222445/151‑1 (MQ=255)                                                        gCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTAt                                                                                           >  2:22543/1‑151 (MQ=255)                                                         cTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATc                                                                                          >  1:105605/1‑151 (MQ=255)                                                           gCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATccc                                                                                        <  1:426063/151‑1 (MQ=255)                                                                ccATGCGCGAGCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCt                                                                                    <  2:521305/150‑1 (MQ=255)                                                                  aTGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATc                                                                                 <  2:335826/151‑1 (MQ=255)                                                                     cgcgACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGc                                                                               >  1:567280/1‑150 (MQ=255)                                                                     cgcgACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCa                                                                              <  2:30892/151‑1 (MQ=255)                                                                           cAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACt                                                                        <  2:141927/151‑1 (MQ=255)                                                                                gctgGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCaa                                                                    <  1:565672/150‑1 (MQ=255)                                                                                   ggCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATg                                                                <  1:30980/151‑1 (MQ=255)                                                                                     cACTGACGAGCGCCGGCCCTGCCGATTACAGCGTCGGCTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCAGCATCGGGGAGGGTGATTAAAACGAGACATCTCGGATATCTTCGGCTATCCCTGCTATCGCGATGACTTCAACAt                                                                 >  1:254797/1‑148 (MQ=255)                                                                                        tGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTa                                                            >  1:73767/1‑150 (MQ=255)                                                                                            gAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCAGCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGGCTTCAACATGCGTACCTg                                                        <  1:316719/150‑1 (MQ=255)                                                                                                gcctgccCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCAcc                                                    <  1:761177/150‑1 (MQ=255)                                                                                                   tgccCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCg                                                   >  1:122022/1‑148 (MQ=255)                                                                                                     cccTGGCGATTACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCGGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGTCTTCAACATGCGGACCTGCACCGAGATc                                              <  1:655633/151‑1 (MQ=255)                                                                                                     cccTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCg                                                   >  1:765636/1‑146 (MQ=255)                                                                                                      ccTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATcc                                             <  1:764452/151‑1 (MQ=255)                                                                                                           cGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCt                                        <  2:90069/151‑1 (MQ=255)                                                                                                              tGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGgc                                     >  1:748653/1‑151 (MQ=255)                                                                                                               gACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGgc                                     <  2:176497/150‑1 (MQ=255)                                                                                                                            gCCCGATTATCGAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCAt                        <  1:553583/150‑1 (MQ=255)                                                                                                                                       gAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCt                                  <  1:664491/129‑1 (MQ=255)                                                                                                                                       gAAAATCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCt                                  >  2:664549/1‑129 (MQ=255)                                                                                                                                        aaacTCTCTCCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCGCGCTGGCGCTGACAGGCCATGAACTg                  >  1:311348/1‑144 (MQ=255)                                                                                                                                               ctcCGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCat    >  1:587187/1‑151 (MQ=255)                                                                                                                                                 ccGACTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCatat  >  1:534932/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                    ACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATAT  >  NC_000913/514228‑514522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑