Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	549,698	A→G	G24G (GGA→GGG)	ylbF →	putative anaerobic allantoin catabolic oxamate carbamoyltransferase; DUF2877 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	549,698	0	A	G	100.0%	175.4 / NA	51	G24G (GGA→GGG)	ylbF	putative anaerobic allantoin catabolic oxamate carbamoyltransferase; DUF2877 family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/24);  total (27/24)

CACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGAGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACGTTGCATCGTCAGGGTAGTGGTTTCGGCCCCGGAGGATGGGTGCTTCGCCGTGCGCAATTCGATGCGTTATGCGGTGGATTATGCGGCAATGAACG  >  NC_000913/549555‑549844                                                                                                                                                |                                                                                                                                                   cACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTggc                                                                                                                                             <  1:295905/151‑1 (MQ=255)        ggCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTGGcggcgg                                                                                                                                        <  1:175676/149‑1 (MQ=255)        ggCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTGGCggcgggc                                                                                                                                      <  1:614040/151‑1 (MQ=255)           atGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGCATTGGTTGAGGCACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTggcg                                                                                                                                            >  1:133299/2‑142 (MQ=255)               tttGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTGGCGGCGGGCGATTAAc                                                                                                                               >  2:739011/1‑151 (MQ=255)                ttGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTGGCGGCGGGCGATTaa                                                                                                                                <  1:274251/149‑1 (MQ=255)                    aCAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTGGCGGCGGGCGATTAACCtg                                                                                                                            >  1:67055/1‑149 (MQ=255)                      aGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTGGCGGCGGGCGATTTACCtgatg                                                                                                                         >  1:548273/1‑150 (MQ=255)                       ggCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGGGGGGGGCGGCGGGGGATTAACCTGATGAc                                                                                                                       >  1:471759/1‑151 (MQ=255)                         cgcTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTGGCGGCGGGCGATTAACCTGATGACgg                                                                                                                     <  2:612778/151‑1 (MQ=255)                             ggAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTGGCGGCGGGCGATTAACCTGATGACGGaaa                                                                                                                  <  1:165428/150‑1 (MQ=255)                             ggAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTGGCGGCGGGCGATTAACCTGATGACGGaa                                                                                                                   <  1:390093/149‑1 (MQ=255)                               aaGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTGGCGGCGGGCGATTATCCTGATGACGGaac                                                                                                                  <  1:520248/148‑2 (MQ=255)                                aGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAgcgg                                                                                                              <  2:211833/151‑1 (MQ=255)                                      ggCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAAc                                                                                                         <  1:739122/150‑1 (MQ=255)                                           aaaGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTa                                                                                                    >  2:70725/1‑150 (MQ=255)                                               cATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACggt                                                                                               >  1:736394/1‑149 (MQ=255)                                               cATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACGtt                                                                                               <  2:688195/151‑1 (MQ=255)                                                aTGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGCTCGCGGGGGTGTGGCGGCGGGGGATTAACCAGATGCCGGGGAGGGGAGAACTGTTAACGTTg                                                                                              >  1:56663/1‑151 (MQ=255)                                                 tGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACGTTg                                                                                              >  1:502888/1‑150 (MQ=255)                                                          gTTGAGGAACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTGGCGGCGGGCGATTAACCTGATGACGGGAAGCGGCGAAATGTTTACGTTGCCTCGGCAgg                                                                                    >  1:376660/1‑151 (MQ=255)                                                               ggAACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACGTTGCATCGTCAGGGTAGt                                                                               <  1:134658/151‑1 (MQ=255)                                                                gAACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACGTTGCATCGTCAGGGTAGTg                                                                              >  2:324943/1‑151 (MQ=255)                                                                gAACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACGTTGCATCGTCAGGGTAGTg                                                                              <  2:290607/151‑1 (MQ=255)                                                                 aaCTCGCAATGACGATCACCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGGTTGTGGCGGCGGGCGATTAACCTTATGTCGGAAAGCGGCGAACTTTTAACGTTGCATCGTCAGCGTAGTgg                                                                             <  1:413570/151‑1 (MQ=255)                                                                      gcAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTGGCGGCGGGCGGTTAACCTGATGACGGAAAGCGGCGAACTGTTAAAGTTGCATCGTCAGGGTAGGGGttt                                                                          >  1:90170/1‑149 (MQ=255)                                                                      gcAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACGTTGCATCGTCAGGGGAGTGGTTTCg                                                                        >  1:30006/1‑151 (MQ=255)                                                                       cAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACGTTGCATCGTCAGGGGAGTGGGTTCgg                                                                       >  1:748362/1‑151 (MQ=255)                                                                                tcatcCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACGTTGCATCGTCAGGGGAGTGGGTTCGGCCCCggagg                                                              >  2:616572/1‑151 (MQ=255)                                                                                 catccatcCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACGTTGCATCGTCAGGGTAGTGGTTTCGGCCCCggagg                                                              >  2:522941/1‑150 (MQ=255)                                                                                    ccatccTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACGTTGCATCGTCAGGGGAGTGGTTTCGGCCCCGGAGGATgg                                                          >  1:96741/1‑151 (MQ=255)                                                                                    ccatccTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACGTTGCATCGTCAGGGGAGTGGTTTCGGCCCCGGAGGATgg                                                          >  2:127979/1‑151 (MQ=255)                                                                                      atccTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACGTTGCATCGTCAGGGTAGTGGTTTCGGCCCCGGAGGATGGGt                                                        <  2:744378/151‑1 (MQ=255)                                                                                       tccTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTGGCGGCGGGCGGTTAACCTGATGACGGAAAGCGGCGAACTGTTAACGTTGCATCGTCAGGGGAGTGGTTTCGGCCCCGGGGGGTggg                                                         >  1:412619/1‑149 (MQ=255)                                                                                             gCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACGTTGCATCGTCAGGGTAGTGGTTTCGGCCCCGGAGGATGGGTGCTTCGc                                                 >  2:399582/1‑151 (MQ=255)                                                                                                  ccAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACGTTGCATCGTCAGGGTAGTGGTTTCGGCCCCGGAGGATGGGTGCTTCGCCGTgc                                            <  1:269721/151‑1 (MQ=255)                                                                                                   cAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTGGCGGCGGGCGATTAACCTGATGACGGGAAGCGGCGAACTGTTAACGTTGCATCGTCAGGGTAGTGGTTTCGGCCCCGGAGGATGGGGGCTTCGCCGTg                                             >  1:302450/1‑149 (MQ=255)                                                                                                   cAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACGTTGCATCGTCAGGGTAGTGGTTTCGGCCCCGGAGGATGGGTGCTTCGCCGTgcg                                           >  2:417505/1‑151 (MQ=255)                                                                                                     gtagtaGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACGTTGCATCGTCAGGGTAGTGGTTTCGGCCCCGGAGGATGGGTGCTTCGCCGTGCGCa                                         <  1:298723/151‑1 (MQ=255)                                                                                                       agtagCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAAAGTTGCATCGTCAGGGGAGTGGTTTCGGCCCCGGAGGGTGGGGTCTTCGCCGTGCGCaa                                        >  1:152447/1‑150 (MQ=255)                                                                                                          agCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACGTTGCATCGTCAGGGTAGTGGTTTCGGTCCCGGAGGATGGGTGCTTCGCCGTGCGCAATTCg                                    <  1:604/151‑1 (MQ=255)                                                                                                            cgcACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACGTTGCATCGTCAGGGTAGTGGTTTCGGCCCCGGAGGATGGGTGCTTCGCCGTGCGCAATTCg                                    >  2:716655/1‑149 (MQ=255)                                                                                                            cgcACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACGTTGCATCGTCAGGGTAGTGGTTTCGGCCCCGGAGGATGGGTGCTTCGCCGTGCGCAATTCGAt                                  <  2:412668/151‑1 (MQ=255)                                                                                                                 cGAATTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACGTTGCATCGTCAGGGTAGTGGTTTCGGCCCCGGAGGATGGGTGCTTCGCCGTGCGCAATTCGATGCGtt                             <  2:147233/151‑1 (MQ=255)                                                                                                                  gAATTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACGTTGCATCGTCAGGGTAGTGGTTTCGGCCCCGGAGGATGGGTGCTTCGCCGTGCGCAATTCGATGCGtt                             <  1:385168/150‑1 (MQ=255)                                                                                                                    aTTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACGTTGCATCGTCAGGGTAGTGGTTTCGGCCCCGGAGGATGGGTGCTTCGCCGTGCGCAATTCGATGCGTTATg                          <  1:202836/151‑1 (MQ=255)                                                                                                                    aTTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACGTTGCATCGTCAGGGTAGTGGTTTCGGCCCCGGAGGATGGGTGCTTCGCCGTGCGCAATTCGATGCGTTATg                          <  2:677457/151‑1 (MQ=255)                                                                                                                      tATCGCCAGTCCTGGCGGTTAGCGGGGGTGTggcg                                                                                                                                            <  2:113489/35‑1 (MQ=255)                                                                                                                      tATCGCCAGTCCTGGCGGTTAGCGGGGGTGTggcg                                                                                                                                            >  1:113457/1‑35 (MQ=255)                                                                                                                                  tGGCGGTTAGCGGGGGGGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACGTTGCATCGTCCGGGGAGTGGGTTCGGGCCCGGAGGCTGGGTGCTTCGCCGTGCGCAATTCGATGCGTTATGCGGTGGGTTTTGCg            >  1:647298/1‑151 (MQ=255)                                                                                                                                     cGGTTAGCGGGGGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACGTTGCATCGTCAGGGTAGTGGTTTCGGCCCCGGAGGATGGGTGCTTCGCCGTGCGCAATTCGATGCGTTATGCGGTGGATTATGCGGc          >  2:89980/1‑150 (MQ=255)                                                                                                                                             ggggGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACGTTGCATCGTCAGGGTAGTGGTTTCGGCCCCGGAGGATGGGTGCTTCGCCGTGCGCAATTCGATGCGTTATGCGGTGGATTATGCGGCAATGAACg  >  2:758823/1‑150 (MQ=255)                                                                                                                                                |                                                                                                                                                   CACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGAGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACGTTGCATCGTCAGGGTAGTGGTTTCGGCCCCGGAGGATGGGTGCTTCGCCGTGCGCAATTCGATGCGTTATGCGGTGGATTATGCGGCAATGAACG  >  NC_000913/549555‑549844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑