Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 613,412 | C→T | P226S (CCC→TCC) | fes → | enterobactin/ferrienterobactin esterase |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 613,412 | 0 | C | T | 98.2% | 202.4 / ‑4.4 | 56 | P226S (CCC→TCC) | fes | enterobactin/ferrienterobactin esterase |
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (28/27); total (28/28) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01 |
AAAGTCACGGCGTGTATGGATTTTTACCACCGGCGATGCAACAGCAGAAGAACGCCCGCTGGCAGTTTTGCTCGATGGCGAATTTTGGGCGCAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTCCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAACTGCCGTGTAATGCGGATTTCTGGCTCGCAGTACAGCAAGAGTTATTACCCCTGGTGAAAGCTATTGCCCCTTTTAGCGATCGTGCCGATCGCA > NC_000913/613264‑613559 | aaaGTCACGGCGTGTATGGATTTTTACCACCGGCGATGCAACAGCAGAAGAACGCCCGCTGGGAGTTTTGCTCGATGGCGGATTTTTGGCGCAAAGGATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCCTCGTCAGCAACttccttcc > 1:671/1‑151 (MQ=255) gCGTGTATGGATTTTTACCACCGGCGATGCAACAGCAGAAGAACGCCCGCTGGCAGTTTTGCTCGATGGCGAATTTTGGGCGCAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTAt > 2:255261/1‑151 (MQ=255) cGTGTATGGATTTTTACCACCGGCGATGCAACAGCAGAAGAACGCCCGCTGGCAGTTTTGCTCGATGGCGAATTTTGGGCGCAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTAtg > 1:387186/1‑151 (MQ=255) cGTGTATGGATTTTTACCACCGGCGATGCAACAGCAGAAGAACGCCCGCTGGCAGTTTTGCTCGATGGCGAATTTTGGGCGCAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTAtg > 1:324157/1‑151 (MQ=255) tATGGATTTTTACCACCGGCGATGCAACAGCAGAAGAACGCCCGCTGGCAGTTTTGCTCGATGGCGAATTTTGGGCGCAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTATGTGtt > 1:141112/1‑151 (MQ=255) ttttACCACCGGCGATGCAACAGCAGAAGAACGCCCGCTGGCAGTTTTGCTCGATGGCGAATTTTGGGCGCAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGa < 1:594753/150‑1 (MQ=255) ccaccGGCGATGCAACAGCAGAAGAACGCCCGCTGGCAGTTTTGCTCGATGGCGAATTTTGGGCGCAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTAt < 2:760367/151‑1 (MQ=255) accGGCGATGCAACAGCAGAAGAACGCCCGCTGGCAGTTTTGCTCGATGGCGAATTTTGGGCGCAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCg > 2:550246/1‑151 (MQ=255) gcGGCGATGCAACAGCAGAAGAACGCCCGCTGGCAGTTTTGCTCGATGGCGAATTTTGGGCGCAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTAt > 1:581530/2‑148 (MQ=255) cGATGCAACAGCAGAAGAACGCCCGCTGGCAGTTTTGCTCGATGGCGAATTTTGGGCGCAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTATGTGtt > 2:450584/1‑132 (MQ=255) cGATGCAACAGCAGAAGAACGCCCGCTGGCAGTTTTGCTCGATGGCGAATTTTGGGCGCAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTATGTGtt < 1:450533/132‑1 (MQ=255) cGATGCAACAGCAGAAGAACGCCCGCTGGCAGTTTTGCTCGATGGCGAATTTTGGGCGCAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGAcacc > 1:761501/1‑151 (MQ=255) gCAACAGCAGAAGAACGCCCGCTGGCAGTTTTGCTCGATGGCGAATTTTGGGCGCAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACg < 2:656993/150‑1 (MQ=255) gCAACAGCAGAAGAACGCCCGCTGGCAGTTTTGCTCGATGGCGAATTTTGGGCGCAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGc > 1:188901/1‑151 (MQ=255) gCAACAGCAGAAGAACGCCCGCTGGCAGTTTTGCTCGATGGCGAATTTTGGGCGCAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCCGACCCATCGTCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACg < 2:653792/150‑1 (MQ=255) cAACAGCAGAAGAACGCCCGCTGGCAGTTTTGCTCGATGGCGAATTTTGGGCGCAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCa < 1:92318/151‑1 (MQ=255) aaCAGCAGAAGAACGCCCGCTGGCAGTTTTGCTCGATGGCGAATTTTGGGCGCAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCAc < 2:241290/151‑1 (MQ=255) cccGCTGGCAGTTTTGCTCGATGGCGAATTTTGGGCGCAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAACTg < 2:473480/151‑1 (MQ=255) cGCTGGCAGTTTTGCTCGATGGCGAATTTTGGGCGCAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAACTGcc > 1:709100/1‑151 (MQ=255) ggCAGTTTTGCTCGATGGCGAATTTTGGGCGCAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAACTGCCgtgt < 2:188934/151‑1 (MQ=255) ggCAGTTTTGCTCGATGGCGAATTTTGGGCGCAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAACTGCCgt < 1:400519/149‑1 (MQ=255) gTTTTGCTCGATGGCGAATTTTGGGCGCAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAACTGCCGTGTAATg < 2:472449/151‑1 (MQ=255) tttGCTCGATGGCGAATTTTGGGCGCAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAACTGCCGTGTAATg > 2:175853/1‑149 (MQ=255) ttGCTCGATGGCGAATTTTGGGCGCAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAACTGCCGTGTAATGCgg > 1:557544/1‑151 (MQ=255) tGCTCGATGGCGAATTTTGGGCGCAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAACTGCCGTGTAATGCgg > 1:375804/1‑150 (MQ=255) gAATTTTGGGCGCAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAACTGCCGTGTAATGCGGATTTCTGGCTCg > 2:520212/1‑151 (MQ=255) aTTTTGGGCGCAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAACTGCCGTGTAATGCGGATTTCTGGCTCGc > 2:104891/1‑150 (MQ=255) ttGGGCGCAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAACTGCCGTGTAATGCGGATTTCTGGCTCGCAGt < 1:15374/150‑1 (MQ=255) gggCGCAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAACTGCCGTGTAATGCGGATTTCTGGCTCGCAGTAc < 1:44338/150‑1 (MQ=255) cgcAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAACTGCCGTGTAATGCGGATTTCTGGCTCGCAGTACAGCa < 2:494330/151‑1 (MQ=255) gcAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAACTGCCGTGTAATGCGGATTTCTGGCTCGCAGTACAGCa > 2:436720/1‑150 (MQ=255) aaaGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAACTGCCGTGTAATGCGGATTTCTGGCTCGCAGTACAGCa > 2:456885/1‑148 (MQ=255) aaaGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAACTGCCGTGTAATGCGGATTTCTGGCTCGCAGTACAGCa > 2:717949/1‑148 (MQ=255) gTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAACTGGCGTGTAATGCGGATTTCTGGCTCGCAGTACAGCAAGAGtt > 2:222487/1‑151 (MQ=255) gTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAACTGCCGTGTAATGCGGATTTCTGGCTCGCAGTACAGCAAGAGtt > 1:584271/1‑151 (MQ=255) aTGCCCGTCTGTCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAACTGCCGTGTAATGCGGATTTCTGGCTCGCAGTACAGCAAGAGttat < 1:222455/151‑1 (MQ=255) aTGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAACTGCCGTGTAATGCGGATTTCTGGCTCGCAGTACAGCAAGAGttat > 2:19634/1‑151 (MQ=255) ccGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAACTGCCGTGTAATGCGGATTTCTGGCTCGCAGTACAGCAAGAGTTATTAcc < 1:132332/151‑1 (MQ=255) ccGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAACTGCCGTGTAATGCGGATTTCTGGCTCGCAGTACAGCAAGAGTTATTAcc > 1:29920/1‑151 (MQ=255) acGTCTAGCGAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGACCACGAACTGCCGTGTAATGCGGATTTCTGGCGCGCAGGACAGCAAGAGttatta > 1:167757/2‑149 (MQ=255) cTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAACTGCCGTGTAATGCGGATTTCTGGCTCGCAGTACAGCAAGAGTTATTACCCCTg > 1:264020/1‑151 (MQ=255) ggCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAACTGCCGTGTAATGCGGATTTCTGGCTCGCAGTACAGCAAGAGTTATTACCCCTgg < 1:737372/150‑1 (MQ=255) aGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAACTGCCGTGTAATGCGGATTTCTGGCTCGCAGTACAGCAAGAGTTATTACCCCTGGTGaa > 1:242689/1‑150 (MQ=255) gCTGACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAACTGCCGTGTAATGCGGATTTCTGGCTCGCAGTACAGCAAGAGTTATTACCCCTGGTGAAAGCt < 2:672400/151‑1 (MQ=255) gCTGACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAACTGCCGTGTAATGCGGATTTCTGGCTCGCAGTACAGCAAGAGTTATTACCCCTGGTGAAAGCt < 1:761714/151‑1 (MQ=255) gACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGTCCACGAACTGCCGTGTAATGCGGATTTCTGGCTCGCAGTACAGCAAGAGTTATTACCCCTGGTGAAAGCTAtt < 1:725907/151‑1 (MQ=255) gACTTCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAACTGCCGTGTAATGCGGATTTCTGGCTCGCAGTACAGCAAGAGTTATTACCCCTGGTGAAAGCTAtt > 2:442995/1‑151 (MQ=255) aCTTCGCTGACCCATCGTCAGCAACTTCCTCCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAACTGCCGTGTAATGCGGATTTCTGGCTCGCAGTACAGCAAGAGTTATTACCCCTGGTGAAAGCTATTg < 2:311488/151‑1 (MQ=255) tCGCTGACCCATCGTCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAACTGCCGTGTAATGCGGATTTCTGGCTCGCAGTACAGCAAGAGTTATTACCCCTGGTGAAAGCTATTGc < 2:745005/149‑1 (MQ=255) gTCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAACTGCCGTGTAATGCGGATTTCTGGCTCGCAGTACAGCAAGAGTTATTACCCCTGGTGAAAGCTATTGCCCCTTTTAg < 1:694667/145‑1 (MQ=255) gTCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAACTGCCGTGTAATGCGGATTTCTGGCTCGCAGTACAGCAAGAGTTATTACCCCTGGTGAAAGCTATTGCCCCTTTTAg > 2:694741/1‑145 (MQ=255) gTCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAACTGCCGTGTAATGCGGATTTCTGGCTCGCAGTACAGCAAGAGTTATTACCCCTGGTGAAAGCTATTGCCCCTTTTAGCGATCg < 2:269362/151‑1 (MQ=255) tCAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAACTGCCGTGTAATGCGGATTTCTGGCTCGCAGTACAGCAAGAGTTATTACCCCTGGTGAAAGCTATTGCCCCTTTTCGCGATCg > 2:64632/1‑150 (MQ=255) cAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAACTGCCGTGTAATGCGGATTTCTGGCTCGCAGTACAGCAAGAGTTATTACCCCTGGTGAAAGCTATTGCCCCTTTTAGCGATCGTg < 1:737295/151‑1 (MQ=255) cAGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAACTGCCGTGTAATGCGGATTTCTGGCTCGCAGTACAGCAAGAGTTATTACCCCTGGTGAAAGCTATTGCCCCTTTTAGCGATCGTg < 2:703595/151‑1 (MQ=255) aGCAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAACTGCCGTGTAATGCGGATTTCTGGCTCGCAGTACAGCAAGAGTTATTACCCCTGGTGAAAGCTATTGCCCCTTTTAGCGATCGTGc < 2:487926/151‑1 (MQ=255) cAACTTCCTTCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAACTGCCGTGTAATGCGGATTTCTGGCTCGCAGTACAGCAAGAGTTATTACCCCTGGTGAAAGCTATTGCCCCTTTTAGCGATCGTGCCg < 2:571859/151‑1 (MQ=255) ccttcCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAACTGCCGTGTAATGCGGATTTCTGGCTCGCAGTACAGCAAGAGTTATTACCCCTGGTGAAAGCTATTGCCCCTTTTAGCGATCGTGCCGATCGCa < 2:341224/151‑1 (MQ=255) | AAAGTCACGGCGTGTATGGATTTTTACCACCGGCGATGCAACAGCAGAAGAACGCCCGCTGGCAGTTTTGCTCGATGGCGAATTTTGGGCGCAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTCCCGCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAACTGCCGTGTAATGCGGATTTCTGGCTCGCAGTACAGCAAGAGTTATTACCCCTGGTGAAAGCTATTGCCCCTTTTAGCGATCGTGCCGATCGCA > NC_000913/613264‑613559 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |