Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	646,110	A→G	P157P (CCT→CCC)	citT ←	citrate/succinate antiporter; citrate carrier

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	646,110	0	A	G	100.0%	193.3 / NA	55	P157P (CCT→CCC)	citT	citrate/succinate antiporter; citrate carrier
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/30);  total (25/30)

TCAGACTGGTACTAATGACCATCATCCACATCAAATAGCCGCCAATACGACGCGCGGACGGATCGTTCGGGAATGATTTAAACAACGGCGGCAGGTTTTTAATGACCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAAGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGACGACCTAACCCGGAAACTTCATACCCTAATGCAAAAATAAATGCGCC  >  NC_000913/645968‑646256                                                                                                                                               |                                                                                                                                                   tCAGACTGGTACTAATGACCATCATCCACATCAAATAGCCGCCAATACGACGCGCGGACGGATCGTTCGGGAATGATTTAAACAACGGCGGCAGGGTTTTAATGACCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAGGGTGTaa                                                                                                                                             >  1:151231/1‑150 (MQ=255)  cAGACTGGTACTAATGACCATCATCCACATCAAATAGCCGCCAATACGACGCGCGGACGGATCGTTCGGGAATGATTTAAACAACGGCGGCAGGTTTTTAATGACCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAGGGTGTaa                                                                                                                                             <  1:560008/149‑1 (MQ=255)   aGACTGGTACTAATGACCATCATCCACATCAAATAGCCGCCAATACGACGCGCGGACGGATCGTTCGGGAATGATTTAAACAACGGCGGCAGGTTTTTAATGACCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAGGGTGTAAACg                                                                                                                                          >  2:126561/1‑151 (MQ=255)      cTGGTACTAATGACCATCATCCACATCAAATAGCCGCCAATACGACGCGCGGACGGATCGTTCGGGAATGATTTAAACAACGGCGGCAGGTTTTTAATGACCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAGGGTGTAAACGGTg                                                                                                                                       >  2:294519/1‑151 (MQ=255)       tGGTACTAAGGACCATCATCCACATCAAATAGCCGCCAATACGACGCGCGGACGGATCGTTCGGGAATGATTTAAACAACGGCGGCAGGTTTTTAATGACCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAGGGTGTAAACGGt                                                                                                                                        <  1:399483/149‑1 (MQ=255)              aaTGACCATCATCCACATCAAATAGCCGCCAATACGACGCGCGGACGGATCGTTCGGGAATGATTTAAACAACGGCGGCAGGTTTTTAATGACCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAGGGTGTAAACGGTGCcagc                                                                                                                                  >  2:286858/1‑148 (MQ=255)              aaTGACCATCATCCACATCAAATAGCCGCCAATACGACGCGCGGACGGATCGTTCGGGAATGATTTAAACAACGGCGGCAGGTTTTTAATGACCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAGGGTGTAAACGGTGCCAGCAGa                                                                                                                               <  2:94835/151‑1 (MQ=255)                 gACCATCATCCACATCAAATAGCCGCCAATACGACGCGCGGACGGATCGTTCGGGAATGATTTAAACAACGGCGGCAGGTTTTTAATGACCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAGGGTGTAAACGGTGCCAGCAGAAt                                                                                                                             <  1:776199/150‑1 (MQ=255)                  aCCATCATCCACATCAAATAGCCGCCAATACGACGCGCGGACGGATCGTTCGGGAATGATTTAAACAACGGCGGCAGGTTTTTAATGACCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAGGGTGTAAACGGTGCCAGCAGAATGt                                                                                                                           >  1:60214/1‑151 (MQ=255)                  aCCATCATCCACATCAAATAGCCGCCAATACGACGCGCGGACGGATCGTTCGGGAATGATTTAAACAACGGCGGCAGGTTTTTAATGACCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAGGGTGTAAACGGTGCCAGCAGAATGt                                                                                                                           <  1:325007/151‑1 (MQ=255)                   ccATCATCCACATCAAATAGCCGCCAATACGACGCGCGGACGGATCGTTCGGGAATGATTTAAACAACGGCGGCAGGTTTTTAATGACCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAGGGTGTAAACGGTGCCAGCAGAAt                                                                                                                             <  1:333806/148‑1 (MQ=255)                        atcCACATCAAATAGCCGCCAATACGACGCGCGGACGGATCGTTCGGGAATGATTTAAACAACGGCGGCAGGTTTTTAATGACCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAGGGTGTAAACGGTGCCAGCAGAATGTCGATa                                                                                                                      >  1:690955/1‑150 (MQ=255)                          ccACATCAAATAGCCGCCAATACGACGCGCGGACGGATCGTTCGGGAATGATTTAAACAACGGCGGCAGGTTTTTAATGACCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAGGGTGTAAACGGTGCCAGCAGAATGTCGATAATg                                                                                                                   <  1:420977/151‑1 (MQ=255)                                 aaaTAGCCGCCAATACGACGCGCGGACGGATCGTTCGGGAATGATTTAAACAACGGCGGCAGGTTTTTAATGACCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAGGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATc                                                                                                             <  1:294486/150‑1 (MQ=255)                                 aaaTAGCCGCCAATACGACGCGCGGACGGATCGTTCGGGAATGATTTAAACAACGGCGGCAGGTTTTTAATGACCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAGGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCg                                                                                                            >  2:417680/1‑151 (MQ=255)                                        cgccAATACGACGCGCGGACGGATCGTTCGGGAATGATTTAAACAACGGCGGCAGGTTTTTAATGACCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAGGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAAcc                                                                                                     >  2:433964/1‑151 (MQ=255)                                            aaTACGACGCGCGGACGGATCGTTCGGGAATGATTTAAACAACGGCGGCAGGTTTTTAATGACCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAGGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAAc                                                                                                 >  2:26270/1‑151 (MQ=255)                                               acgacgCGCGGACGGATCGTTCGGGAATGATTTAAACAACGGCGGCAGGTTTTTAATGACCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAGGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGt                                                                                               <  2:172986/150‑1 (MQ=255)                                               acgacgCGCGGACGGATCGTTCGGGAATGATTTAAACAACGGCGGCAGGTTTTTAATGACCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAGGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGt                                                                                               <  1:111311/150‑1 (MQ=255)                                               acgacgCGCGGACGGATCGTTCGGGAATGATTTAAACAACGGCGGCAGGTTTTTAATGACCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAGGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTc                                                                                              <  1:555134/151‑1 (MQ=255)                                                cgacgCGCGGACGGATCGTTCGGGAATGATTTAAACAACGGCGGCAGGTTTTTAATGACCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAGGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCa                                                                                             <  1:38874/151‑1 (MQ=255)                                                      gcgGACGGATCGTTCGGGAATGATTTAAACAACGGCGGCAGGTTTTTAATGACCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAGGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAgcgtg                                                                                        <  1:54988/150‑1 (MQ=255)                                                       cggacggaTCGTTCGGGAATGATTTAAACAACGGCGGCAGGTTTTTAATGACCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAGGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAgcgt                                                                                         >  1:619912/1‑148 (MQ=255)                                                         gacggaTCGTTCGGGAATGATTTAAACAACGGCGGCAGGTTTTTAATGACCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAGGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGtt                                                                                    >  2:234537/1‑151 (MQ=255)                                                          acggaTCGTTCGGGAATGATTTAAACAACGGCGGCAGGTTTTTAATGACCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAGGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGttt                                                                                   <  1:737075/151‑1 (MQ=255)                                                               tCGTTCGGGAATGATTTAAACAACGGCGGCAGGTTTTTAATGACCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAGGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGccc                                                                               >  2:588111/1‑150 (MQ=255)                                                                cGTTCGGGAATGATTTAAACAACGGCGGCAGGTTTTTAATGACCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAGGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCAt                                                                             >  2:143499/1‑151 (MQ=255)                                                                   tCGGGAATGATTTAAACAACGGCGGCAGGTTTTTAATGACCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAGGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGa                                                                           <  1:724906/150‑1 (MQ=255)                                                                     gggAATGATTTAAACAACGGCGGCAGGTTTTTAATGACCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAGGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAAtt                                                                        <  2:151264/151‑1 (MQ=255)                                                                               tAAACAACGGCGGCAGGTTTTTAATGACCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAGGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAgg                                                                >  1:677811/1‑149 (MQ=255)                                                                                   caacGGCGGCAGGTTTTTAATGACCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAGGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTc                                                                      <  2:670156/139‑1 (MQ=255)                                                                                   caacGGCGGCAGGTTTTTAATGACCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAGGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTc                                                                      >  1:670098/1‑139 (MQ=255)                                                                                    aacGGCGGCAGGTTTTTAATGACCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAGGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAggg                                                         <  2:124489/151‑1 (MQ=255)                                                                                      cggcggCAGGTTTTTAATGACCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAGGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCa                                                       <  1:701980/151‑1 (MQ=255)                                                                                                 ttttAATGACCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAGGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCgacgac                                             <  1:55035/150‑1 (MQ=255)                                                                                                   ttAATGACCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAGGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGACGACCt                                           >  2:398490/1‑150 (MQ=255)                                                                                                     aaTGACCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAGGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGACGACCTaa                                         <  1:433913/150‑1 (MQ=255)                                                                                                     aaTGACCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAGGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGACGACCTAAc                                        <  2:397511/151‑1 (MQ=255)                                                                                                      aTGACCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAGGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGACGACCTAAcc                                       >  2:166732/1‑151 (MQ=255)                                                                                                       tGACCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAGGGTGAAAACGGTGCCAGCAGAATGTCGATAATGACTATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGACGACCTAAcc                                       >  2:423055/1‑150 (MQ=255)                                                                                                               aaaaCCGTACCCCCGGTACGCGCGGTGTTGGAGGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGACGACCTAACCCGGAAACtt                              >  1:59301/1‑151 (MQ=255)                                                                                                                aaaCCGTACCCCCGGTACGCGCGGTGTTGGAGGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGACGACCTAACCCGGAAACTTc                             <  1:749863/151‑1 (MQ=255)                                                                                                                    cGTACCCCCGGTACGCGCGGTGTTGGAGGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGACGACCTAACCCGGAAACTTCATAc                         >  2:658012/1‑151 (MQ=255)                                                                                                                    cGTACCCCCGGTACGCGCGGTGTTGGAGGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGACGACCTAACCCGGAAACTTCATAc                         >  1:399457/1‑151 (MQ=255)                                                                                                                       aCCCCCGGTACGCGCGGTGTTGGAGGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGACGACCTAACCCGGAAACTTCATACCCt                      >  1:133296/1‑151 (MQ=255)                                                                                                                        cccccGGTACGCGCGGTGTTGGAGGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGACGACCTAACCCGGAAACTTCATACCCTa                     <  1:234504/151‑1 (MQ=255)                                                                                                                           ccGGTACGCGCGGTGTTGGAGGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGACGACCTAACCCGGAAACTTCATACCCTAATg                  <  1:756898/151‑1 (MQ=255)                                                                                                                           ccGGTACGCGCGGTGTTGGAGGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGACGACCTAACCCGGAAACTTCATACCCTAATg                  <  1:749871/151‑1 (MQ=255)                                                                                                                                aCGCGCGGTGTTGGAGGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGACGACCTAACCCGGAAACTTCATACCCTAATGCaaa              <  1:271756/150‑1 (MQ=255)                                                                                                                                aCGCGCGGTGTTGGAGGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGACGACCTAACCCGGAAACTTCATACCCTAATGCaaa              >  2:44033/1‑150 (MQ=255)                                                                                                                                  gcgcgGTGTTGGAGGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGACGACCTAACCCGGAAACTTCATACCCTAATGCAAaaat           <  1:272487/151‑1 (MQ=255)                                                                                                                                     cgGTGTTGGAGGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGACGACCTAACCCGGAAACTTCATACCCTAATGCAAaaataaa        <  2:43590/151‑1 (MQ=255)                                                                                                                                        tgtTGGAGGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGACGACCTAACCCGGAAACTTCATACCCTAATGCAAAAATAAATgc     >  1:561924/1‑151 (MQ=255)                                                                                                                                         gtTGGAGGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGACGACCTAACCCGGAAACTTCATACCCTAATGCAAAAATAAATgc     <  2:465292/150‑1 (MQ=255)                                                                                                                                            ggAGGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGACGACCTAACCCGGAAACTTCATACCCTAATGCAAAAATAAATGCGcc  >  2:460206/1‑150 (MQ=255)                                                                                                                                               |                                                                                                                                                   TCAGACTGGTACTAATGACCATCATCCACATCAAATAGCCGCCAATACGACGCGCGGACGGATCGTTCGGGAATGATTTAAACAACGGCGGCAGGTTTTTAATGACCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAAGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGACGACCTAACCCGGAAACTTCATACCCTAATGCAAAAATAAATGCGCC  >  NC_000913/645968‑646256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑