Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	684,342	G→A	A24V (GCC→GTC)	rihA ←	ribonucleoside hydrolase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	684,342	0	G	A	100.0%	141.0 / NA	38	A24V (GCC→GTC)	rihA	ribonucleoside hydrolase 1
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/22);  total (16/22)

GCATTAACGGTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGAAACGATA  >  NC_000913/684196‑684487                                                                                                                                                   |                                                                                                                                                  gCATTAACGGTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAgacgag                                                                                                                                               <  2:381382/151‑1 (MQ=255)     tAACGGTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAgacgagggcg                                                                                                                                           >  1:382820/1‑151 (MQ=255)      aaCGGTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAgacgagggcg                                                                                                                                           >  2:413789/1‑150 (MQ=255)            tttACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACt                                                                                                                                     <  1:588009/150‑1 (MQ=255)               aCCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTg                                                                                                                                 <  2:492334/151‑1 (MQ=255)                   cgccgcCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGAt                                                                                                                             <  2:100199/151‑1 (MQ=255)                      cgccTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAg                                                                                                                           >  2:500905/1‑150 (MQ=255)                           gCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGcgtcg                                                                                                                      <  1:186914/150‑1 (MQ=255)                                cGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGa                                                                                                                <  2:652834/151‑1 (MQ=255)                                    atatCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTg                                                                                                            <  2:316322/151‑1 (MQ=255)                                    atatCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTg                                                                                                            <  2:549764/151‑1 (MQ=255)                                                ttAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCt                                                                                                 >  2:602475/1‑150 (MQ=255)                                                  aaGCAAGGTCAGCATACGCAGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTa                                                                                                <  1:142815/149‑1 (MQ=255)                                                     cAAGGTCAGCATACGCAGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAAc                                                                                              >  2:1323/1‑148 (MQ=255)                                                        ggTCAGCATACGCAGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAAtt                                                                                        >  2:719820/1‑151 (MQ=255)                                                                 aCGCAGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGcc                                                                               >  1:766761/1‑151 (MQ=255)                                                                  cGCAGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCa                                                                              <  1:388832/151‑1 (MQ=255)                                                                  cGCAGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCa                                                                              <  2:447386/151‑1 (MQ=255)                                                                              cGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCtt                                                                  >  1:185542/1‑151 (MQ=255)                                                                                 aaGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCttgtt                                                               >  2:494683/1‑151 (MQ=255)                                                                                    gTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTtgtg                                                            >  2:137047/1‑151 (MQ=255)                                                                                          tCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGcttc                                                      >  2:597792/1‑151 (MQ=255)                                                                                             ggTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCttt                                                   <  1:690150/151‑1 (MQ=255)                                                                                              gtgtCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTg                                                  <  1:265330/151‑1 (MQ=255)                                                                                                gtCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTtgttgt                                                             >  1:104770/1‑138 (MQ=255)                                                                                                gtCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTtgttgt                                                             <  2:104802/138‑1 (MQ=255)                                                                                                gtCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCg                                                <  1:238205/151‑1 (MQ=255)                                                                                                        tCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAggg                                         >  1:60368/1‑150 (MQ=255)                                                                                                         ccGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTa                                       <  1:542191/151‑1 (MQ=255)                                                                                                           ggcggAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAAc                                     <  2:236366/151‑1 (MQ=255)                                                                                                                     gTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGt                           >  2:761550/1‑151 (MQ=255)                                                                                                                     gTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGt                           >  2:661283/1‑151 (MQ=255)                                                                                                                     gTAATTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGt                           <  2:100219/151‑1 (MQ=255)                                                                                                                        aTTGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAAc                        >  1:52355/1‑151 (MQ=255)                                                                                                                         ttGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAAc                        >  1:456013/1‑150 (MQ=255)                                                                                                                          tGCTTTGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACa                       <  1:211262/150‑1 (MQ=255)                                                                                                                              ttGACATCAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGAt                    <  2:194425/149‑1 (MQ=255)                                                                                                                                     cAAGCTCTGGTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGc           <  2:677273/151‑1 (MQ=255)                                                                                                                                             ggTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGAAAc      <  2:693198/148‑1 (MQ=255)                                                                                                                                             ggTGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGAAACg     <  2:252381/149‑1 (MQ=255)                                                                                                                                               tGAGACGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGAAACGATa  >  2:337816/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                                  GCATTAACGGTTTTACCGCGCCGCCTGCTACCGGAATATCGGTGCGATTAAGCAAGGTCAGCATACGCAGAACATTGCGTAAGGTTTTTTCTGGTGTCTGGTTTCCGGCGGAAGACGTAATTGCTTTGACATCAAGCTCTGGTGAGGCGAGGGCGAGAACTATTGCGATAGCGTCGTCATGACCTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGAAACGATA  >  NC_000913/684196‑684487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑