Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	699,862	A→G	A155A (GCT→GCC)	umpH ←	UMP phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	699,862	0	A	G	100.0%	152.0 / NA	41	A155A (GCT→GCC)	umpH	UMP phosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/16);  total (25/16)

CAGGTTATCGCCGACAATCACCGTTTCTTCCGAATGCGCCTGCATTTTGTTTAATGCTGCGCGGATGATCCACGGGCTGGGCTTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGG  >  NC_000913/699718‑700007                                                                                                                                                 |                                                                                                                                                  cAGGTTATCGCCGACAATCACCGTTTCTTCCGAATGCGCCTGCATTTTGTTTAATGCTGCGCGGATGATCCACGGGCTGGGCTTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAGGCggg                                                                                                                                              >  2:12522/1‑150 (MQ=255)   ggTTATCGCCGACAATCACCGTTTCTTCCGAATGCGCCTGCATTTTGTTTAATGCTGCGCGGATGATCCACGGGCTGGGCTTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAGGCggg                                                                                                                                              >  2:9719/1‑148 (MQ=255)      tATCGCCGACAATCACCGTTTCTTCCGAATGCGCCTGCATTTTGTTTAATGCTGCGCGGATGATCCACGGGCTGGGCTTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAGGCGGGATaaa                                                                                                                                         <  1:549120/150‑1 (MQ=255)       aTCGCCGACAATCACCGTTTCTTCCGAATGCGCCTGCATTTTGTTTAATGCTGCGCGGATGATCCACGGGCTGGGCTTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAGGCGGGATaaaa                                                                                                                                        >  1:467980/1‑150 (MQ=255)            cGACAATCACCGTTTCTTCCGAATGCGCCTGCATTTTGTTTAATGCTGCGCGGATGATCCACGGGCTGGGCTTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAGGCGGGATAAAAACcgcg                                                                                                                                  >  2:276554/1‑151 (MQ=255)                       gTTTCTTCCGAATGCGCCTGCATTTTGTTTAATGCTGCGCGGATGATCCACGGGCTGGGCTTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAGGCGGGATAAAAACCGCGCCCGTGGgtgt                                                                                                                       <  2:207538/151‑1 (MQ=255)                         ttcttcCGAATGCGCCTGCATTTTGTTTAATGCTGCGCGGATGATCCACGGGCTGGGCTTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAGGCGGGGTAAAAACCCCGcccccaggggtcc                                                                                                                     >  1:23306/1‑141 (MQ=255)                                     cgcCTGCATTTTGTTTAATGCTGCGCGGATGATCCACGGGCTGGGCTTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAGGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCg                                                                                                         >  2:467496/1‑151 (MQ=255)                                       ccTGCATTTTGTTTAATGCTGCGCGGATGATCCACGGGCTGGGCTTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAGGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCg                                                                                                         >  2:210852/1‑149 (MQ=255)                                           cATTTTGTTTAATGCTGCGCGGATGATCCACGGGCTGGGCTTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAGGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATaaa                                                                                                    >  1:159585/1‑150 (MQ=255)                                            aTTTTGTTTAATGCTGCGCGGATGATCCACGGGCTGGGCTTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAGGCGGGATAAAAACCGCGCCCGTGGGGGTCCGGATTGGTGGCGATaaaa                                                                                                   >  2:57321/1‑150 (MQ=255)                                            aTTTTGTTTAATGCTGCGCGGATGATCCACGGGCTGGGCTTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAGGCGGGATAAAAACCGCGCCCGTGGGGGTCCGGATTGGTGGCGATaaaa                                                                                                   >  1:146648/1‑150 (MQ=255)                                             ttttGTTTAATGCTGCGCGGATGATCCACGGGCTGGGCTTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAGGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATaaaa                                                                                                   >  1:5151/1‑149 (MQ=255)                                              tttGTTTAATGCTGCGCGGATGATCCACGGGCTGGGCTTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAGGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATaaaa                                                                                                   >  2:536164/1‑148 (MQ=255)                                               ttGTTTAATGCTGCGCGGATGATCCACGGGCTGGGCTTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAGGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGt                                                                                                <  1:266083/150‑1 (MQ=255)                                                   ttAATGCTGCGCGGATGATCCACGGGCTGGGCTTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAGGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCAc                                                                                            <  1:83529/150‑1 (MQ=255)                                                    tAATGCTGCGCGGATGATCCACGGGCTGGGCTTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAGGCGGGATAAAAACCGCGCCCGTGGGGGTCCGGATTGGTGGCGATAAAACGTGCAcc                                                                                           >  2:121247/1‑150 (MQ=255)                                                          tgcGCGGATGATCCACGGGCTGGGCTTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAGGCGGGATAAAAACCCCGCCCGTGGGGGTCCGGGTTGGGGGCGATAAAACGTGCACCGTTAGc                                                                                     >  1:57232/1‑150 (MQ=255)                                                                   gatCCACGGGCTTGGCTTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAGGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAg                                                                           <  1:237161/151‑1 (MQ=255)                                                                        aCGGGCTGGGCTTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAGGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGgctgc                                                                      <  2:424342/151‑1 (MQ=255)                                                                          gggctgggctTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAGGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCtt                                                                    <  1:194301/151‑1 (MQ=255)                                                                            gctgggctTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAGGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAg                                                                                      <  2:85081/131‑1 (MQ=255)                                                                            gctgggctTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAGGCGGGATAAAAACCGCGCCCGTGGGGGTCCGGATTGGTGGCGGTAAAACGTGCACCGTTAg                                                                                      >  1:85050/1‑131 (MQ=255)                                                                              tgggctTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAGGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTAt                                                                 >  2:388265/1‑150 (MQ=255)                                                                              tgggctTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAGGCGGGATAAAAAACGCGCCCGTGGGGGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCCGCTTTAt                                                                 >  2:702765/1‑150 (MQ=255)                                                                                ggctTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAGGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGc                                                               >  1:220227/1‑150 (MQ=255)                                                                                     aCCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAGGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATg                                                         <  2:153858/151‑1 (MQ=255)                                                                                        aaCATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAGGCGGGATAAAAACCCCGCCCGTGGGTGTGCGGGTTGGTGGCGATAAAACGTGGACCGTTAGGGACGAAAAAGGCTGCTTTATGCATCATGt                                                        >  2:497941/1‑149 (MQ=255)                                                                                                aCGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAGGCGGGATAAAAAACGCGCCCGTGGGGGTCCGGGTTGGGGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTTTTCATCATGTCCCAGTTGt                                               >  1:573259/1‑150 (MQ=255)                                                                                                       gcgcCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAGGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAAcg                                        <  2:176250/150‑1 (MQ=255)                                                                                                         gcCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAGGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGt                                     >  1:152324/1‑151 (MQ=255)                                                                                                                 aTTTTCTCAATCCCTGCACACAACGCGCCACAGGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCcaa                             <  1:746987/151‑1 (MQ=255)                                                                                                                       tcAATCCCTGCACACAACGCGCCACAGGCGGGATAAAAACCGCGCCCCTGGGTGCCCGGAGTGGGGGCGATAAAACGTGCACCGTTAGCGACGAAAAAGGCTGCTTTATGCCACATGTCCCAGCTGTAGGAACCCGCTTCGCcaaca                           >  1:389179/1‑147 (MQ=255)                                                                                                                        cAATCCCTGCACACAACGCGCCACAGGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCAc                      <  2:269111/151‑1 (MQ=255)                                                                                                                            cccTGCACACAACGCGCCACAGGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGaaa                  <  1:14902/151‑1 (MQ=255)                                                                                                                               tGCACACAACGCGCCACAGGCGGGATAAAAACCGCGCCCGTGGGGGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATc                >  1:446248/1‑150 (MQ=255)                                                                                                                                  acacaACGCGCCACAGGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAggg            <  1:673286/151‑1 (MQ=255)                                                                                                                                        cgcgCCACAGGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTcaca      <  1:326316/151‑1 (MQ=255)                                                                                                                                        cgcgCCACAGGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTcaca      <  1:270738/151‑1 (MQ=255)                                                                                                                                          cgcCACAGGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATc    >  2:499875/1‑151 (MQ=255)                                                                                                                                             cacaGGCGGGATAAAAACCCCGCCCGTGGGTTTCCCGGTTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACCCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCgg  >  1:150645/1‑150 (MQ=255)                                                                                                                                                 |                                                                                                                                                  CAGGTTATCGCCGACAATCACCGTTTCTTCCGAATGCGCCTGCATTTTGTTTAATGCTGCGCGGATGATCCACGGGCTGGGCTTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGG  >  NC_000913/699718‑700007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑