Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	732,614	A→G	D1011G (GAC→GGC)	rhsC →	Rhs protein with putative toxin domain; putative neighboring cell growth inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	732,614	0	A	G	100.0%	82.7 / NA	37	D1011G (GAC→GGC)	rhsC	Rhs protein with putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/21);  total (16/21)

CGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTATGGCGGCGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGCCTGACCACGATACAGAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTC  >  NC_000913/732469‑732750                                                                                                                                                  |                                                                                                                                         cgcACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTATGGCGGCGTGAACGGGGCCTg                                                                                                                                      >  2:616779/1‑150 (MQ=25)          cGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTATGGCGGCGTGAACGGGGCCTGACGGGCTGGa                                                                                                                            <  2:27949/151‑1 (MQ=18)                           tCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTATGGCGGCGTGAACGGGGCCTGACGGGCTGGATGTCGCTGTCACGGaa                                                                                                            >  1:494794/1‑150 (MQ=18)                              gCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTATGGCGGCGTGAACGGGGCCTGACGGGCTGGATGTCGCTGTCACGGAAACCg                                                                                                        <  1:244860/151‑1 (MQ=18)                                aCCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGGGGCAAAACGGGTATGGCGGCGTGAACGGGGCCTGACGGGCTGGATGTCGCTGTCACGGAAACCg                                                                                                        >  1:270729/1‑149 (MQ=18)                                           cACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTATGGCGGCGTGAACGGGGCCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTgg                                                                                            >  1:144351/1‑150 (MQ=18)                                           cACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTATGGCGGCGTGAACGGGGCCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGt                                                                                           <  2:178752/151‑1 (MQ=18)                                                cacGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTATGGCGGCGTGAACGGGGCCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACgg                                                                                       <  1:496871/150‑1 (MQ=18)                                                 acGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTATGGCGGCGTGAACGGGGCCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCt                                                                                     <  2:654033/151‑1 (MQ=18)                                                           aaTATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTATGGCGGCGTGAACGGGGCCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCg                                                                            >  2:359337/1‑150 (MQ=18)                                                            atatGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTATGGCGGCGTGAACGGGGCCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGa                                                                           >  1:359316/1‑150 (MQ=18)                                                                 cAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTATGGCGGCGTGAACGGGGCCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGCCt                                                                     <  2:517846/151‑1 (MQ=21)                                                                        gCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTATGGCGGCGTGAACGGGGCCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGCCTGACCa                                                                >  1:665871/1‑149 (MQ=18)                                                                        gCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTATGGCGGCGTGAACGGGGCCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGCCTGACCACg                                                              <  1:435172/151‑1 (MQ=18)                                                                               gAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTATGGCGGCGTGAACGGGGCCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGCCTGACCACGATACAGa                                                       <  2:551857/151‑1 (MQ=18)                                                                                aaaGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTATGGCGGCGTGAACGGGGCCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGCCTGACCACGATACAGaa                                                      <  2:359236/151‑1 (MQ=18)                                                                                 aaGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTATGGCGGCGTGAACGGGGCCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGCCTGACCACGATACAGaa                                                      >  1:589619/1‑150 (MQ=18)                                                                                 aaGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTATGGCGGCGTGAACGGGGCCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGCCTGACCACGATACAGaa                                                      >  1:626590/1‑150 (MQ=18)                                                                                 aaGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTATGGCGGCGTGAACGGGGCCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGCCTGACCACGATACAGAAc                                                     <  1:151216/151‑1 (MQ=18)                                                                                     cGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTATGGCGGCGTGAACGGGGCCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGCCTGACCACGATACAGAACGAc                                                  <  2:3688/150‑1 (MQ=18)                                                                                         aTCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTATGGCGGCGTGAACGGGGCCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGCCTGACCACGATACAGAACGACAGaa                                              >  1:527322/1‑150 (MQ=18)                                                                                            tttACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTATGGCGGCGTGAACGGGGCCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGCCTGACCACGATACAGAACGACAGAAccc                                           >  2:224382/1‑150 (MQ=18)                                                                                              tACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTATGGCGGCGTGAACGGGGCCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGCCTGACCACGATACAGAACGACAGAACCCGc                                         >  2:518912/1‑150 (MQ=18)                                                                                                cGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTATGGCGGCGTGAACGGGGCCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGCCTGACCACGATACAGAACGACAGAACCCGCATc                                      <  2:522930/151‑1 (MQ=18)                                                                                                    ccGCTGGGCCGCAGGGTGGCAAAACGGGTATGGCGGCGTGAACGGGGCCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGCCTGACCACGATACAGAACGACAGAACCCGCATcc                                     >  1:361666/1‑148 (MQ=18)                                                                                                     cGCTGGGCCGCAGGGTGGCAAAACGGGTATGGCGGCGTGAACGGGGCCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGCCTGACCACGATACAGAACGACAGAACCCGCATCCAGAc                                 <  2:687470/151‑1 (MQ=18)                                                                                                       cTGGGCCGCAGGGTGGCAAAACGGGTATGGCGGCGTGAACGGGGCCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGCCTGACCACGATACAGAACGACAGAACCCGCATCCAGACg                                >  2:486234/1‑150 (MQ=18)                                                                                                            ccGCAGGGTGGCAAAACGGGTATGGCGGCGTGAACGGGGCCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGCCTGACCACGATACAGAACGACAGAACCCGCATCCAGACGATTTAt                          <  1:189799/151‑1 (MQ=18)                                                                                                                 gggTGGCAAAACGGGTATGGCGGCGTGAACGGGGCCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGCCTGACCACGATACAGAACGACAGAACCCGCATCCAGACGATTTATCAGcc                     <  2:231725/151‑1 (MQ=18)                                                                                                                  ggTGGCAAAACGGGTATGGCGGCGGGAACGGGGCCTGACGGGCTGGATGTCGCTGTCGCGGCAACCGCAAGTGACCTGGTACGGCTGGGCCGGCGACCGCCTGACCACGATACAGAACGACAGAACCCGCATCCAGACGATTTATCAGc                      <  1:351213/149‑1 (MQ=255)                                                                                                                   gTGGCAAAACGGGTATGGCGGCGTGAACGGGGCCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGCCTGACCACGATACAGAACGACAGAACCCGCATCCAGACGATTTATCAGCCgg                   <  1:569255/151‑1 (MQ=18)                                                                                                                   gTGGCAAAACGGGTATGGCGGCGTGAACGGGGCCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGCCTGACCACGATACAGAACGACAGAACCCGCATCCAGACGATTTATCAGCCgg                   <  1:239477/151‑1 (MQ=18)                                                                                                                       cAAAACGGGTATGGCGGCGTGAACGGGGCCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGCCTGACCACGATACAGAACGACAGAACCCGCATCCAGACGATTTATCAGCCgggg                 >  2:12507/1‑149 (MQ=18)                                                                                                                        aaaaCGGGTATGGCGGCGTGAACGGGGCCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGCCTGACCACGATACAGAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAg               <  1:649228/150‑1 (MQ=18)                                                                                                                             gggTATGGCGGCGTGAACGGGGCCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGAGCGCCTGACCACGATACAGAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCAc         <  1:319921/151‑1 (MQ=18)                                                                                                                              ggcaTGGCGGCGTGAACGGGGCCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGCCTGACCACGATACAGAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACg        <  2:257118/148‑1 (MQ=17)                                                                                                                                     cggcgTGAACGGGGCCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGCCTGACCACGATACAGAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACtc  >  2:90966/1‑150 (MQ=14)                                                                                                                                                  |                                                                                                                                         CGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTATGGCGGCGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGCCTGACCACGATACAGAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTC  >  NC_000913/732469‑732750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑