Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	768,591	T→C	V205A (GTG→GCG)	mngB →	alpha‑mannosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	768,591	0	T	C	100.0%	252.0 / NA	71	V205A (GTG→GCG)	mngB	alpha‑mannosidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/42);  total (29/42)

GGTACTGATAAAACCGAGTTTTTGTGGCAAAGCAGTGACGGTAGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGTGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCAACGTAAGTTTGTGATGAGCCGCTTTGA  >  NC_000913/768446‑768732                                                                                                                                                  |                                                                                                                                              ggTACTGATAAAACCGAGTTTTTGTGGCAAAGCAGTGACGGTAGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTgg                                                                                                                                          <  2:318193/151‑1 (MQ=255)       gATAAAACCGAGTTTTTGTGGCAAAGCAGTGACGGTAGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGaaaa                                                                                                                                      <  2:466275/149‑1 (MQ=255)       gATAAAACCGAGTTTTTGTGGCAAAGCAGTGACGGTAGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGaaaa                                                                                                                                      >  2:212664/1‑149 (MQ=255)        aTAAAACCGAGTTTTTGTGGCCAAGAAGTGACGGTAGCGAAGTGACGACGCAGGGGCTGGCGCTGGGCTACGCCATCGGTAAGTACTTACATGCCGACGAAAACGGATTACGTAAACGCCTAGACAGTTATTTTGACGCGCTGGAAAAAGc                                                                                                                                   >  1:684688/1‑151 (MQ=255)          aaacCCGAGTTTTTGTGGCAAAGCAGTGACGGTAGCGAAGTGACGGCGCAGGTGCTGCCGCTGTGCTACGCCATCTGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCg                                                                                                                                  <  1:524004/150‑1 (MQ=255)              ccGAGTTTTTGTGGCAAAGCAGTGACGGTAGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGt                                                                                                                             >  2:287057/1‑151 (MQ=255)                 aGTTTTTGTGGCAAAGCAGTGACGGTAGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTaa                                                                                                                           <  1:536260/150‑1 (MQ=255)                    ttttGTGGCAAAGCAGTGACGGTAGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCa                                                                                                                        <  1:128189/150‑1 (MQ=255)                    ttttGTGGCAAAGCAGTGACGGTAGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCa                                                                                                                        <  2:507375/150‑1 (MQ=255)                      ttGTGGCAAAGCAGTGACGGTAGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCaaa                                                                                                                      <  1:194378/150‑1 (MQ=255)                        gtgGCAAAGCAGTGACGGTAGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAaga                                                                                                                    <  2:168528/150‑1 (MQ=255)                          ggCAAAGCATTGACGGTAGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTAACGCGCTGGAAAAAGCGTCTGTAACCAAAgaga                                                                                                                  <  1:96135/150‑1 (MQ=255)                              aaGCAGTGACGGTAGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTtg                                                                                                             >  1:426183/1‑151 (MQ=255)                                 cAGTGACGGTAGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTtgctg                                                                                                          <  2:291213/151‑1 (MQ=255)                                     gACGGTAGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCg                                                                                                       >  1:244223/1‑150 (MQ=255)                                        ggTAGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATg                                                                                                   <  2:655554/151‑1 (MQ=255)                                            gCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATgggaa                                                                                               <  2:330531/151‑3 (MQ=255)                                              gAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATg                                                                                             <  1:635448/151‑1 (MQ=255)                                                aGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGAc                                                                                           <  2:495137/151‑1 (MQ=255)                                                   gACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGAcc                                                                                          >  1:432430/1‑149 (MQ=255)                                                   gACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAg                                                                                        <  2:401080/151‑1 (MQ=255)                                                      ggCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATg                                                                                     <  2:741531/151‑1 (MQ=255)                                                        cgcAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGcc                                                                                   >  2:462363/1‑151 (MQ=255)                                                         gcAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCa                                                                                  <  2:693086/151‑1 (MQ=255)                                                           aGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCAtt                                                                                <  2:291629/151‑1 (MQ=255)                                                                    cGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCaaa                                                                        >  1:64039/1‑150 (MQ=255)                                                                       tGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAatat                                                                    >  2:575229/1‑151 (MQ=255)                                                                        gggCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAatat                                                                    <  2:233614/150‑1 (MQ=255)                                                                         ggCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCt                                                                  >  1:198234/1‑151 (MQ=255)                                                                          gCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCtt                                                                 <  2:657726/151‑1 (MQ=255)                                                                            tACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCg                                                               >  1:620119/1‑151 (MQ=255)                                                                              cGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGaa                                                             <  2:299789/151‑1 (MQ=255)                                                                               gCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAg                                                            <  2:194336/151‑1 (MQ=255)                                                                                ccATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGt                                                           <  1:768992/151‑1 (MQ=255)                                                                                  aTCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGa                                                         <  2:752923/151‑1 (MQ=255)                                                                                      gTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGa                                                     <  1:318220/151‑1 (MQ=255)                                                                                        aaGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATa                                                   <  1:398644/151‑1 (MQ=255)                                                                                         aGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGAt                                                    <  1:295763/149‑1 (MQ=255)                                                                                         aGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGAt                                                    >  2:295796/1‑149 (MQ=255)                                                                                          gTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAg                                                 <  1:596944/151‑1 (MQ=255)                                                                                             cTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCt                                               >  2:379639/1‑150 (MQ=255)                                                                                             cTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCt                                               >  2:72732/1‑150 (MQ=255)                                                                                               tACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTAc                                             <  1:471741/150‑1 (MQ=255)                                                                                               tACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTAc                                             <  2:321916/150‑1 (MQ=255)                                                                                               tACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACg                                            >  2:38710/1‑151 (MQ=255)                                                                                                    gCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTgag                                        <  1:748981/150‑1 (MQ=255)                                                                                                    gCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTga                                         <  1:608057/149‑1 (MQ=255)                                                                                                     ccGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGAt                                      <  2:404300/151‑1 (MQ=255)                                                                                                      cgacgaAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTg                                          >  1:704667/1‑146 (MQ=255)                                                                                                          gaAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTAcc                                 >  1:600129/1‑151 (MQ=255)                                                                                                            aaaCGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCt                               >  1:34299/1‑151 (MQ=255)                                                                                                            aaaCGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCt                               <  2:713751/151‑1 (MQ=255)                                                                                                              aCGGATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCa                             <  2:288736/151‑1 (MQ=255)                                                                                                                ggATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCAAc                           >  2:630132/1‑151 (MQ=255)                                                                                                                 gATTACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCAACg                          <  1:261090/151‑1 (MQ=255)                                                                                                                    tACGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCAACGTa                        <  2:9854/150‑1 (MQ=255)                                                                                                                     aCGTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCAACGTa                        >  1:183940/1‑149 (MQ=255)                                                                                                                       gTAAACGCCTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCAACGTAAGtt                    <  1:457711/151‑1 (MQ=255)                                                                                                                              ccTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCAACGTAAGTTTGtgat              <  2:391389/150‑1 (MQ=255)                                                                                                                               cTCGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCAACGTAAGTTTGtgatg             >  1:392601/1‑150 (MQ=255)                                                                                                                                tCGACAGTTATTTTGAGGCGCTGGAAAAAGCGTCTGTAGCCAAAGAGAATTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGCGGTGATGGATAAGCTACGTGAGATCTACCCTCAACGTCGGTTTGtgatg             >  1:54217/1‑149 (MQ=255)                                                                                                                                 cGACAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCAACGTAAGTTTGtggtga            <  1:303237/149‑1 (MQ=255)                                                                                                                                   aCAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCAACGTAAGTTTGTGATGAGCCg        >  1:758998/1‑151 (MQ=255)                                                                                                                                   aCAGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCAACGTAAGTTTGTGATGAGCCg        >  1:30476/1‑151 (MQ=255)                                                                                                                                     aGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCAACGTAAGTTTGTGATGAGCCGCt      <  1:689482/151‑1 (MQ=255)                                                                                                                                     aGTTATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCAACGTAAGTTTGTGATGAGCCGCt      >  1:424508/1‑151 (MQ=255)                                                                                                                                        tATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCAACGTAAGTTTGTGATGAGCCGCttt    <  2:424559/150‑1 (MQ=255)                                                                                                                                        tATTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCAACGTAAGTTTGTGATGAGCCGCTTTg   >  2:167243/1‑151 (MQ=255)                                                                                                                                         aTTTTGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCAACGTAAGTTTGTGATGAGCCGCTTTg   >  2:24353/1‑150 (MQ=255)                                                                                                                                           tttGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCAACGTAAGTTTGTGATGAGCCGCTTTGa  >  1:690517/1‑149 (MQ=255)                                                                                                                                            ttGACGCGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGCCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCAACGTAAGTTTGTGATGAGCCGCTTTGa  >  1:110410/1‑148 (MQ=255)                                                                                                                                                  |                                                                                                                                              GGTACTGATAAAACCGAGTTTTTGTGGCAAAGCAGTGACGGTAGCGAAGTGACGGCGCAGGTGCTGCCGCTGGGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGACAGTTATTTTGACGTGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGGCATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATCTACCCTCAACGTAAGTTTGTGATGAGCCGCTTTGA  >  NC_000913/768446‑768732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑