Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	811,250	A→G	A290A (GCA→GCG)	bioF →	8‑amino‑7‑oxononanoate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	811,250	0	A	G	100.0%	163.4 / NA	48	A290A (GCA→GCG)	bioF	8‑amino‑7‑oxononanoate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/25);  total (23/25)

CGGGGCAGCGGTGCTTTGCTCCAGTACGGTGGCGGATTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCACGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGTGCGTTACAACTGGCAGAAAAACTGCGT  >  NC_000913/811103‑811397                                                                                                                                                    |                                                                                                                                                    cGGGGCAGCGGTGCTTTGCTCCAGTACGGTGGCGGATTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCgg                                                                                                                                                  <  1:31345/151‑1 (MQ=255)  ggggCAGCGGTGCTTTGCTCCAGTACGGTGGCGGATTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGGGATGAGGGGGATGCGCgg                                                                                                                                                  >  1:133803/1‑150 (MQ=255)        gCGGTGCTTTGCTCCAGTACGGTGGCGGATTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGaa                                                                                                                                            >  2:196185/1‑150 (MQ=255)             gCTTTGCTCCAGTACGGTGGCGGATTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACt                                                                                                                                       <  1:470716/150‑1 (MQ=255)                     ccAGTACGGTGGCGGATTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCAc                                                                                                                               >  1:768655/1‑150 (MQ=255)                      cAGTACGGTGGCGGATTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTc                                                                                                                             >  2:402542/1‑151 (MQ=255)                      cAGTACGGTGGCGGATTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGGGATGCGCGGCGCGAAAAACTGGCGGCACt                                                                                                                              >  1:665647/1‑150 (MQ=255)                       aGTACGGTGGCGGATTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCa                                                                                                                            <  1:753067/151‑1 (MQ=255)                           cGGTGGCGGATTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCAtt                                                                                                                          <  1:164049/149‑1 (MQ=255)                             gTGGCGGATTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTAcgc                                                                                                                      <  1:229929/151‑1 (MQ=255)                              tGGCGGATTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTAcgcg                                                                                                                     >  1:716082/1‑151 (MQ=255)                                gCGGATTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGtt                                                                                                                   >  2:745840/1‑151 (MQ=255)                                 cGGATTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGttt                                                                                                                  <  1:776827/151‑1 (MQ=255)                                  ggATTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGgttt                                                                                                                 >  1:643773/1‑151 (MQ=255)                                    aTTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTc                                                                                                                >  2:431739/1‑150 (MQ=255)                                    aTTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCg                                                                                                               <  1:726571/151‑1 (MQ=255)                                         ctgctgCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGTGTTTTCGTGCCg                                                                                                          >  2:366213/1‑151 (MQ=255)                                              gcAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGt                                                                                                      >  2:16443/1‑150 (MQ=255)                                                   tCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAgg                                                                                                 <  1:35505/150‑1 (MQ=255)                                                    cgcccgccACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGAt                                                                                               <  2:260246/151‑1 (MQ=255)                                                                  tCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCCCTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGttt                                                                                      >  2:589533/1‑146 (MQ=255)                                                                   cTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCtt                                                                                >  2:84518/1‑151 (MQ=255)                                                                   cTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCACTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGGACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCtt                                                                                >  1:368784/1‑151 (MQ=255)                                                                    tACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCtt                                                                                >  1:390664/1‑150 (MQ=255)                                                                    tACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCtt                                                                                >  2:254262/1‑150 (MQ=255)                                                                     aCAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTg                                                                               <  2:768994/150‑1 (MQ=255)                                                                     aCAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTg                                                                               <  2:698932/150‑1 (MQ=255)                                                                           ccAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTc                                                                        <  2:197572/151‑1 (MQ=255)                                                                             aGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCAt                                                                      >  2:464961/1‑151 (MQ=255)                                                                             aGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCAt                                                                      <  1:535293/151‑1 (MQ=255)                                                                                  gccgccCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAgc                                                                 <  2:144301/151‑1 (MQ=255)                                                                                    cgcccgcTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAgcgc                                                               <  2:229666/151‑1 (MQ=255)                                                                                     gcccgcTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGcc                                                              <  1:123640/151‑1 (MQ=255)                                                                                      cccgcTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCa                                                             <  1:741963/151‑1 (MQ=255)                                                                                         gcTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATcc                                                          <  1:545897/151‑1 (MQ=255)                                                                                              ggCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCa                                                     <  2:622493/151‑1 (MQ=255)                                                                                                 gcAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCattg                                                  >  2:617479/1‑151 (MQ=255)                                                                                                  cAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCattg                                                  >  2:56286/1‑150 (MQ=255)                                                                                                   aGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCattgat                                                <  1:500925/151‑1 (MQ=255)                                                                                                           cGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTg                                        <  2:49604/151‑1 (MQ=255)                                                                                                                gTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAAc                                   <  1:335408/151‑1 (MQ=255)                                                                                                                    cTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGcc                               >  1:416173/1‑151 (MQ=255)                                                                                                                      ggCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGt                             >  1:504146/1‑151 (MQ=255)                                                                                                                       gCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGTg                            <  2:138824/151‑1 (MQ=255)                                                                                                                            cATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGTGCGTTa                       <  1:6733/151‑1 (MQ=255)                                                                                                                                      gatgaGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGTGCGTTACAACTGGCa              <  2:224024/150‑1 (MQ=255)                                                                                                                                          aGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGTGCGTTACAACTGGCAGaaa          >  2:78657/1‑150 (MQ=255)                                                                                                                                                 tGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGTGCGTTACAACTGGCAGAAAAACTGCGt  >  2:31733/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                    CGGGGCAGCGGTGCTTTGCTCCAGTACGGTGGCGGATTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCACGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGTGCGTTACAACTGGCAGAAAAACTGCGT  >  NC_000913/811103‑811397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑