Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	832,662	T→C	M97V (ATG→GTG)	ybiA ←	DUF1768 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	832,662	0	T	C	100.0%	216.7 / NA	61	M97V (ATG→GTG)	ybiA	DUF1768 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/32);  total (29/32)

GTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCATCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTT  >  NC_000913/832518‑832808                                                                                                                                                 |                                                                                                                                                   gTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTg                                                                                                                                              <  2:190347/151‑1 (MQ=255)   cGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGtt                                                                                                                                            <  1:365849/151‑1 (MQ=255)    gATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTc                                                                                                                                           >  2:184712/1‑151 (MQ=255)     aTTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCt                                                                                                                                          >  1:491163/1‑151 (MQ=255)     aTTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCt                                                                                                                                          >  2:389525/1‑151 (MQ=255)                   cATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCa                                                                                                                            <  2:156749/151‑1 (MQ=255)                   cATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCa                                                                                                                            <  2:362347/151‑1 (MQ=255)                       accaccGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGttt                                                                                                                        >  2:753813/1‑151 (MQ=255)                         caccGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGttttt                                                                                                                      <  2:499737/151‑1 (MQ=255)                           ccGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTAc                                                                                                                    >  1:200130/1‑151 (MQ=255)                                 ccccAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCagag                                                                                                              <  2:125252/151‑1 (MQ=255)                                 ccccAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCa                                                                                                                 <  1:758608/148‑1 (MQ=255)                                  cccAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAgg                                                                                                             <  2:748619/151‑1 (MQ=255)                                   ccAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGc                                                                                                            >  1:191295/1‑151 (MQ=255)                                      gTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTa                                                                                                         <  1:584451/151‑1 (MQ=255)                                          gCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAAc                                                                                                     >  1:153859/1‑151 (MQ=255)                                           cATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACg                                                                                                    <  2:162298/151‑1 (MQ=255)                                           cATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACg                                                                                                    <  1:158857/151‑1 (MQ=255)                                              cGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATc                                                                                                 >  2:58072/1‑151 (MQ=255)                                               gTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATcg                                                                                                <  2:217493/151‑1 (MQ=255)                                                                  aGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTgcggcg                                                                              >  2:655831/1‑150 (MQ=255)                                                                          gggCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAggg                                                                     >  1:105608/1‑151 (MQ=255)                                                                            gcgcGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAgggg                                                                    >  1:521304/1‑150 (MQ=255)                                                                              gcgGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGga                                                                   >  1:209211/1‑149 (MQ=255)                                                                                gggtGCCAGCAAGAGCGCACGCAGTTCTGCATTCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGCGCGGCGACCCTAGGGgaaga                                                                >  1:22327/4‑150 (MQ=255)                                                                                    gCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAAcc                                                            <  1:316901/150‑1 (MQ=255)                                                                                            gagCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGAtc                                                   <  1:701257/151‑1 (MQ=255)                                                                                             agCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGAtc                                                   >  1:78760/1‑150 (MQ=255)                                                                                                gcacgcaGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCtt                                               <  2:748476/151‑1 (MQ=255)                                                                                                   cgcaGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTcgc                                            >  2:345825/1‑151 (MQ=255)                                                                                                   cgcaGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTcgc                                            <  1:58041/151‑1 (MQ=255)                                                                                                   cgcaGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTcgc                                            <  1:185783/151‑1 (MQ=255)                                                                                                    gcaGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTcgcg                                           <  1:188338/151‑1 (MQ=255)                                                                                                      aGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGt                                         >  2:368187/1‑151 (MQ=255)                                                                                                       gTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGt                                         >  2:472343/1‑150 (MQ=255)                                                                                                            gCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTAtt                                      <  2:563241/148‑1 (MQ=255)                                                                                                            gCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTAtt                                      <  1:56093/148‑1 (MQ=255)                                                                                                             cATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTtc                                  >  1:380530/1‑151 (MQ=255)                                                                                                              aTGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTtc                                  >  2:462765/1‑150 (MQ=255)                                                                                                                gCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTtcatc                               <  1:32537/151‑1 (MQ=255)                                                                                                                gCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTtcat                                <  2:463968/150‑1 (MQ=255)                                                                                                                   gTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCaa                             <  2:758196/150‑1 (MQ=255)                                                                                                                   gTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAg                            <  2:744488/151‑1 (MQ=255)                                                                                                                     tCGAATTTAGGGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCGAGTTTTTACGCAGAGGCTTAGAACGATCGCGACCCATGCGTGCGGCGCCCATATGGGAAGAAACCCGACGGATCCATTCGCGGTATTTTTCAGCaa                             >  1:641078/1‑148 (MQ=255)                                                                                                                     tCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAgg                           <  2:162119/150‑1 (MQ=255)                                                                                                                     tCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAgg                           >  1:573091/1‑150 (MQ=255)                                                                                                                       gAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAAt                        >  2:535944/1‑151 (MQ=255)                                                                                                                        aaTTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAAtt                       >  1:567480/1‑151 (MQ=255)                                                                                                                            tAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAAttttt                    <  2:528947/150‑1 (MQ=255)                                                                                                                            tAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTtg                   >  2:630950/1‑151 (MQ=255)                                                                                                                             aGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTtgt                  >  2:169707/1‑151 (MQ=255)                                                                                                                              gcgcGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTtgtg                 <  2:699758/151‑1 (MQ=255)                                                                                                                                  gAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTg             <  2:713483/151‑1 (MQ=255)                                                                                                                                    aGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGaa           <  1:753738/151‑1 (MQ=255)                                                                                                                                       gCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAAt        >  2:283643/1‑151 (MQ=255)                                                                                                                                       gCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAAt        >  1:370986/1‑151 (MQ=255)                                                                                                                                        cTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAAt        >  2:172920/1‑150 (MQ=255)                                                                                                                                          tttCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATa       <  1:244436/149‑1 (MQ=255)                                                                                                                                          tttCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAgt     >  1:499979/1‑151 (MQ=255)                                                                                                                                           ttCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAgt     <  2:545190/150‑1 (MQ=255)                                                                                                                                             cGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGtt  <  2:159292/151‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                   GTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCATCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTT  >  NC_000913/832518‑832808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑