Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	904,502	T→C	intergenic (‑35/‑91)	ybjP ← / → ybjQ	lipoprotein/UPF0145 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	904,502	0	T	C	100.0%	179.2 / NA	49	intergenic (‑35/‑91)	ybjP/ybjQ	lipoprotein/UPF0145 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/22);  total (27/22)

ACGCAAGGACCACTGCGTGTGCCGTTATCTTTATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGTTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGAATATTGCGGT  >  NC_000913/904358‑904649                                                                                                                                                 |                                                                                                                                                    aCGCAAGGACCACTGCGTGTGCCGTTATCTTTATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACg                                                                                                                                               >  1:33046/1‑151 (MQ=255)   gCAAGGACCACTGCGTGTGCCGTTATCTTTATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGat                                                                                                                                             <  2:469677/151‑1 (MQ=255)    cAAGGACCACTGCGTGTGCCGTTATCTTTATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACg                                                                                                                                               <  2:557797/148‑1 (MQ=255)       ggACCACTGCGTGTGCCGTTATCTTTATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGataata                                                                                                                                         <  2:153922/151‑1 (MQ=255)         aCCACTGCGTGTGCCGTTATCTTTATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATaataat                                                                                                                                       <  2:655184/151‑1 (MQ=255)             cTGCGTGTGCCGTTATCTTTATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGat                                                                                                                                    >  1:458599/1‑150 (MQ=255)                               ttATAAGCTGGAGTGACTGTGGTGCTGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCg                                                                                                                  <  2:14772/150‑1 (MQ=255)                               ttATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAg                                                                                                                    <  2:77257/148‑1 (MQ=255)                                tataAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGt                                                                                                                 <  1:254835/150‑1 (MQ=255)                                 ataAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTAt                                                                                                               >  2:452623/1‑151 (MQ=255)                                    aGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAg                                                                                                            <  1:748582/151‑1 (MQ=255)                                        ggAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTc                                                                                                          <  1:293544/149‑1 (MQ=255)                                        ggAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCt                                                                                                         <  2:387108/150‑1 (MQ=255)                                              aCTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATg                                                                                                  <  1:350270/151‑1 (MQ=255)                                                      gCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTga                                                                                           <  2:60691/150‑1 (MQ=255)                                                       cAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTgt                                                                                           <  1:569528/149‑2 (MQ=255)                                                                aggagCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCt                                                                                <  2:618032/151‑1 (MQ=255)                                                                aggagCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCt                                                                                <  1:31809/151‑1 (MQ=255)                                                                aggagCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCt                                                                                >  1:303419/1‑151 (MQ=255)                                                                  gagCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCtt                                                                               >  2:38542/1‑150 (MQ=255)                                                                  gagCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTa                                                                              <  1:772135/151‑1 (MQ=255)                                                                   agCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCtt                                                                               >  2:487009/1‑149 (MQ=255)                                                                      aGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCa                                                                           >  1:161530/1‑150 (MQ=255)                                                                      aGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCa                                                                           >  2:90123/1‑150 (MQ=255)                                                                         gcgcAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACa                                                                       <  2:354195/151‑1 (MQ=255)                                                                             aGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAgg                                                                    >  2:455577/1‑150 (MQ=255)                                                                             aGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAgg                                                                    >  1:197147/1‑150 (MQ=255)                                                                                    aaGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAgatcgat                                                            >  2:245470/1‑151 (MQ=255)                                                                                          tGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGc                                                       >  1:90124/1‑150 (MQ=255)                                                                                           gTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCaa                                                     <  2:550938/151‑1 (MQ=255)                                                                                           gTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCa                                                      >  2:354236/1‑150 (MQ=255)                                                                                               aTTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAAtttt                                                 >  2:751689/1‑151 (MQ=255)                                                                                               aTTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAAtttt                                                 >  1:323884/1‑151 (MQ=255)                                                                                                     tGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACa                                            >  2:335276/1‑150 (MQ=255)                                                                                                         gcgcATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCaa                                                                          <  2:205129/116‑1 (MQ=255)                                                                                                         gcgcATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCaa                                                                          >  1:205097/1‑116 (MQ=255)                                                                                                           gcATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACTATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCaa                                     <  2:371812/151‑1 (MQ=255)                                                                                                             aTAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAAct                                   >  2:183168/1‑151 (MQ=255)                                                                                                                     ttCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAg                            <  2:748447/150‑1 (MQ=255)                                                                                                                       ccTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGcc                         >  1:545966/1‑151 (MQ=255)                                                                                                                       ccTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGcc                         >  1:355325/1‑151 (MQ=255)                                                                                                                              aGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCAt                  >  1:405491/1‑151 (MQ=255)                                                                                                                                cGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCg                >  2:562321/1‑151 (MQ=255)                                                                                                                                 gATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGt               >  2:622435/1‑151 (MQ=255)                                                                                                                                     gCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGa            >  1:308786/1‑150 (MQ=255)                                                                                                                                        ctGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGAat          <  2:343685/149‑1 (MQ=255)                                                                                                                                         tGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGAATAtt       >  2:618203/1‑151 (MQ=255)                                                                                                                                          gAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGAATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGAATAtt       >  1:520513/1‑150 (MQ=255)                                                                                                                                            aaGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGAATATTGCg    >  1:559218/1‑151 (MQ=255)                                                                                                                                              gcgcTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGAATATTGCGgt  <  2:5199/151‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                    ACGCAAGGACCACTGCGTGTGCCGTTATCTTTATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGTTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGAATATTGCGGT  >  NC_000913/904358‑904649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑