Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	944,703	G→A	G224D (GGT→GAT)	dmsC →	dimethyl sulfoxide reductase, anaerobic, subunit C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	944,703	0	G	A	100.0%	151.4 / NA	40	G224D (GGT→GAT)	dmsC	dimethyl sulfoxide reductase, anaerobic, subunit C
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/20);  total (20/20)

TGCGTCTGCTGCCAGCAATTTCTGTACTGGCACTGGTAGTGAGTGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGGTGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTCTGGCAGGGGAATTAATCGGTCGCGGCGT  >  NC_000913/944574‑944850                                                                                                                                  |                                                                                                                                                    tGCGTCTGCTGCCAGCAATTTCTGTACTGGCACTGGTAGTGAGTGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGAt                                                                                                                                >  2:93721/1‑151 (MQ=255)   cGTCTGCTGCCAGCAATTTCTGTACTGGCACTGGTAGTGAGTGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCg                                                                                                                              >  2:712386/1‑151 (MQ=255)             cAGCAATTTCTGTACTGGCACTGGTAGTGAGTGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGc                                                                                                                    >  1:185850/1‑151 (MQ=255)                 aaTTTCTGTACTGGCACTGGTAGTGAGTGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGt                                                                                                                 >  2:480749/1‑150 (MQ=255)                        gTACTGGCACTGGTAGTGAGTGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCtgt                                                                                                         >  1:659502/1‑151 (MQ=255)                            tGGCACTGGTAGTGAGTGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCtt                                                                                                     >  2:190288/1‑151 (MQ=255)                             ggCACTGGTAGTGAGTGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTtg                                                                                                    >  2:499788/1‑151 (MQ=255)                                   ggTAGTGAGTGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGa                                                                                                <  1:138764/149‑1 (MQ=255)                                   ggTAGTGAGTGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGAtt                                                                                              <  1:583829/151‑1 (MQ=255)                                               cGTGGTGTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCt                                                                                   >  2:51918/1‑150 (MQ=255)                                                gtggtgTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTa                                                                                  >  1:585884/1‑150 (MQ=255)                                                  ggtgTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTaaaa                                                                               <  2:335023/151‑1 (MQ=255)                                                   gtgtCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAg                                                                              <  1:23349/151‑1 (MQ=255)                                                     gtCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGt                                                                            <  2:685354/151‑1 (MQ=255)                                                     gtCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGt                                                                            <  1:263572/151‑1 (MQ=255)                                                            aTGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGc                                                                     <  2:185991/151‑1 (MQ=255)                                                                aGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGc                                                                 >  2:324659/1‑151 (MQ=255)                                                                aGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGc                                                                 <  2:228074/151‑1 (MQ=255)                                                                      cAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTcc                                                           >  1:440131/1‑151 (MQ=255)                                                                      cAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTcc                                                           <  1:689045/151‑1 (MQ=255)                                                                      cAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTcc                                                           <  2:177919/151‑1 (MQ=255)                                                                          gCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCt                                                        >  2:530507/1‑150 (MQ=255)                                                                            tGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACt                                                     >  2:338105/1‑151 (MQ=255)                                                                                gACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCt                                                 <  2:75835/151‑1 (MQ=255)                                                                                          aGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTAtcattc                                        >  2:130790/1‑150 (MQ=255)                                                                                                gtgCAGCAGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCattcattc                                    <  1:171966/148‑1 (MQ=255)                                                                                                  gcagcaGGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCtt                               <  1:195997/151‑1 (MQ=255)                                                                                                   cagcagGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTc                              >  1:758848/1‑151 (MQ=255)                                                                                                   cagcagGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTc                              <  1:425170/151‑1 (MQ=255)                                                                                                    agcagGCCGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTCt                             <  1:226610/151‑1 (MQ=255)                                                                                                           cGCAGCGCTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTCTGGCAggg                      >  2:636415/1‑151 (MQ=255)                                                                                                                cgcTGGTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTCTGGCAGGGGAAtt                 <  2:26705/151‑1 (MQ=255)                                                                                                                    ggTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTCTGGCAGGGGAATTAATc             <  2:114494/151‑1 (MQ=255)                                                                                                                     gTGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTCTGGCAGGGGAATTAATCg            <  1:211685/151‑1 (MQ=255)                                                                                                                      tGCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTCTGGCAGGGGAATTAATCgg           >  2:515909/1‑151 (MQ=255)                                                                                                                       gCCGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTCTGGCAGGGGAATTAATCGGt          <  2:28044/151‑1 (MQ=255)                                                                                                                        ccGGACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTCTGGCAGGGGAATTAATCGGTc         <  1:50631/151‑1 (MQ=255)                                                                                                                          ggACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTCTGGCAGGGGAATTAATCGGTcgc       >  1:3478/1‑151 (MQ=255)                                                                                                                          ggACTATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTCTGGCAGGGGAATTAATCGGTcgc       >  1:218274/1‑151 (MQ=255)                                                                                                                              tATGATGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTCTGGCAGGGGAATTAATCGGTCgcggcg   >  1:706598/1‑151 (MQ=255)                                                                                                                               atgatgCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTCTGGCAGGGGAATTAATCGGTCGCGGCGt  >  2:710219/1‑151 (MQ=255)                                                                                                                                  |                                                                                                                                                    TGCGTCTGCTGCCAGCAATTTCTGTACTGGCACTGGTAGTGAGTGGCGTGGTGTCAGTGATGCAGGGCGCAGAGCTGGCGACTATTCATAGTTCTGTGCAGCAGGCCGCAGCGCTGGTGCCGGACTATGGTGCGCTGATGTCCTGGCGGATCGTGCTTTTGGCCGTTGCCCTGTGCTTGTGGATTGCACCACAGCTAAAAGGTTATCAGCCTGCGGTTCCGCTACTTTCTGTATCATTCATTCTGCTTCTGGCAGGGGAATTAATCGGTCGCGGCGT  >  NC_000913/944574‑944850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑