Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,022,227	A→G	Y553H (TAT→CAT)	yccS ←	putative transporter, FUSC superfamily inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,022,227	0	A	G	100.0%	211.0 / NA	58	Y553H (TAT→CAT)	yccS	putative transporter, FUSC superfamily inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/31);  total (27/31)

GCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCTACTTCAAAACCTT  >  NC_000913/1022083‑1022373                                                                                                                                                 |                                                                                                                                                   gCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACa                                                                                                                                              <  2:549841/151‑1 (MQ=255)  cGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGAc                                                                                                                                               >  1:753313/1‑149 (MQ=255)  cGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGAc                                                                                                                                               >  1:728095/1‑149 (MQ=255)   ggggTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGt                                                                                                                                            <  2:272910/151‑1 (MQ=255)      gTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTgg                                                                                                                                         >  2:277845/1‑151 (MQ=255)          cGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCctc                                                                                                                                     <  1:46294/151‑1 (MQ=255)           gTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCctct                                                                                                                                    >  1:427005/1‑151 (MQ=255)           gTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCctct                                                                                                                                    <  1:444501/151‑1 (MQ=255)             tCGGCTCGCTGCACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGTCCTCtg                                                                                                                                   <  1:93641/150‑1 (MQ=255)                 cTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGgcg                                                                                                                              >  2:85695/1‑151 (MQ=255)                      tGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTc                                                                                                                         >  2:272389/1‑151 (MQ=255)                       ggACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTc                                                                                                                         <  2:489371/150‑1 (MQ=255)                           atatTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCa                                                                                                                    <  2:717521/151‑1 (MQ=255)                             atTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATg                                                                                                                  >  1:717835/1‑151 (MQ=255)                             atTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATg                                                                                                                  <  2:210397/151‑1 (MQ=255)                                tGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATgcgc                                                                                                               >  1:213309/1‑151 (MQ=255)                                tGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATgcg                                                                                                                <  2:673923/150‑1 (MQ=255)                                 gATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGcgcg                                                                                                              >  2:361395/1‑151 (MQ=255)                                  aTACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCgg                                                                                                             >  1:281478/1‑151 (MQ=255)                                      caccGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGc                                                                                                            >  1:504297/1‑148 (MQ=255)                                       accGACGCCAGCTCAGCATCGCGGTTGTGTGCATCGCGGCTGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGAt                                                                                                        <  2:749574/151‑1 (MQ=255)                                               cAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAAc                                                                                                <  2:504349/151‑1 (MQ=255)                                                     aGCTTCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCATGACGGTGATCACGACCCTGATGGTATTGCTCCCGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGcc                                                                                            >  1:497692/1‑149 (MQ=255)                                                      gCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGt                                                                                         >  2:468661/1‑151 (MQ=255)                                                      gCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGt                                                                                         >  2:709333/1‑151 (MQ=255)                                                       cATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTc                                                                                        <  2:752837/151‑1 (MQ=255)                                                          cACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAgg                                                                                     >  1:424367/1‑151 (MQ=255)                                                          cACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAg                                                                                      <  2:408271/150‑1 (MQ=255)                                                                 gtgtgCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGAt                                                                               <  2:712710/150‑1 (MQ=255)                                                                   gtgCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGt                                                                             <  2:55496/150‑1 (MQ=255)                                                                   gtgCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTa                                                                            <  2:552421/151‑1 (MQ=255)                                                                       aTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTc                                                                        <  2:728169/151‑1 (MQ=255)                                                                         cgcgGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCAc                                                                      <  2:628339/151‑1 (MQ=255)                                                                         cgcgGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCAc                                                                      <  1:277812/151‑1 (MQ=255)                                                                           cggcggGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTg                                                                    >  1:596736/1‑151 (MQ=255)                                                                                ggCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTgccgcc                                                               >  1:114256/1‑151 (MQ=255)                                                                                    atacgatacgCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACg                                                           >  2:716811/1‑151 (MQ=255)                                                                                      acgatacgCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGc                                                          <  2:281511/150‑1 (MQ=255)                                                                                      acgatacgCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGAGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGc                                                          <  2:126824/150‑1 (MQ=255)                                                                                               cAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGcaca                                                 >  2:353505/1‑150 (MQ=255)                                                                                                    ggTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAAt                                           <  2:101282/151‑1 (MQ=255)                                                                                                       tATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCa                                         <  2:425295/150‑1 (MQ=255)                                                                                                          cACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCg                                      <  1:496889/150‑1 (MQ=255)                                                                                                          cACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGt                                     >  1:566013/1‑151 (MQ=255)                                                                                                            cGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTAt                                   <  1:468610/151‑1 (MQ=255)                                                                                                                ccTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGAt                               <  2:296703/151‑1 (MQ=255)                                                                                                                 cTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGAtt                              >  1:251313/1‑151 (MQ=255)                                                                                                                     tGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTAcgc                          >  2:700378/1‑151 (MQ=255)                                                                                                                     tGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTAcgc                          >  2:360942/1‑151 (MQ=255)                                                                                                                      ggTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTAcgcg                         <  2:71194/151‑1 (MQ=255)                                                                                                                             cTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAAc                  >  2:69351/1‑151 (MQ=255)                                                                                                                               ccAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGc                >  2:611750/1‑151 (MQ=255)                                                                                                                                cAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCt               <  1:391180/151‑1 (MQ=255)                                                                                                                                 aGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCTa              <  1:700304/151‑1 (MQ=255)                                                                                                                                          tCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCTACTTCAAAAc     >  1:717591/1‑151 (MQ=255)                                                                                                                                             agaTGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGcaca                                                 <  2:7174/104‑1 (MQ=255)                                                                                                                                             agaTGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGcaca                                                 >  1:7146/1‑104 (MQ=255)                                                                                                                                              gaTGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCTACTTCAAAACCtt  <  1:228631/150‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                   GCGGGGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCTACTTCAAAACCTT  >  NC_000913/1022083‑1022373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑