Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,028,234	A→G	T97A (ACT→GCT)	yccU →	putative CoA‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,028,234	0	A	G	100.0%	196.2 / NA	54	T97A (ACT→GCT)	yccU	putative CoA‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/28);  total (26/28)

GCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAACTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATCCCTCGCCTGGGCCTGGCCAAATAAAAAATCCCCGGAAGGCAAAA  >  NC_000913/1028096‑1028379                                                                                                                                           |                                                                                                                                                  gCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGc                                                                                                                                       >  1:365059/1‑151 (MQ=255)  ccGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGc                                                                                                                                       >  1:177364/1‑150 (MQ=255)  ccGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCa                                                                                                                                      <  2:592961/151‑1 (MQ=255)  ccGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCa                                                                                                                                      <  1:480891/151‑1 (MQ=255)   cGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCaa                                                                                                                                     <  1:275347/151‑1 (MQ=255)   cGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCa                                                                                                                                      >  2:675951/1‑150 (MQ=255)       aaaaCGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACt                                                                                                                                   >  2:461106/1‑149 (MQ=255)       aaaaCGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACt                                                                                                                                   <  1:461055/149‑1 (MQ=255)         aaCGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTgggg                                                                                                                               <  1:356200/151‑1 (MQ=255)          aCGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTggggg                                                                                                                              >  2:41170/1‑151 (MQ=255)           cGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGt                                                                                                                             <  1:481601/151‑1 (MQ=255)            gctgctGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTg                                                                                                                            >  1:319419/1‑151 (MQ=255)              tgctgGGCCAGCAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGAt                                                                                                                          >  1:49821/1‑151 (MQ=255)                  gggCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAAt                                                                                                                      >  2:609082/1‑151 (MQ=255)                            gggTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCgg                                                                                                            >  2:11023/1‑151 (MQ=255)                                  ggCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTa                                                                                                       <  2:476572/150‑1 (MQ=255)                                    cACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTg                                                                                                    >  1:527333/1‑151 (MQ=255)                                     aCGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTgg                                                                                                   <  2:540833/151‑1 (MQ=255)                                          ggCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACg                                                                                               <  2:594459/150‑1 (MQ=255)                                                         aaaaGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAAt                                                                               <  2:182811/151‑1 (MQ=255)                                                         aaaaGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAAt                                                                               >  1:764811/1‑151 (MQ=255)                                                             gTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATgtgg                                                                           >  2:431397/1‑151 (MQ=255)                                                               cGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATgtggtg                                                                         >  1:99488/1‑151 (MQ=255)                                                               cGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATgtggtg                                                                         <  1:58783/151‑1 (MQ=255)                                                               cGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATgtggtg                                                                         <  1:216070/151‑1 (MQ=255)                                                               cGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATgtggt                                                                          <  2:136544/150‑1 (MQ=255)                                                                 atatGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGAt                                                                       >  1:653902/1‑151 (MQ=255)                                                                   atGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATg                                                                      <  1:748884/150‑1 (MQ=255)                                                                           tGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCATCTGGGGGTGATTAATGAGCAAGCGTCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCt                                                               <  2:385619/149‑1 (MQ=255)                                                                            gTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGcc                                                            <  2:332560/151‑1 (MQ=255)                                                                              tttCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGccc                                                           <  1:360200/150‑1 (MQ=255)                                                                                 ccGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGc                                                        <  1:128661/150‑1 (MQ=255)                                                                                 ccGTAATTCAGAAGCGGCGGGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGGACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCt                                                       <  1:555826/151‑1 (MQ=255)                                                                                   gTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTAt                                                     >  2:263829/1‑151 (MQ=255)                                                                                      aTTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTAtt                                                    >  1:504018/1‑149 (MQ=255)                                                                                             aGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATccc                                            >  1:6031/1‑150 (MQ=255)                                                                                                ggcgtggggcgtggCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATCCCTCgc                                        >  1:484295/1‑151 (MQ=255)                                                                                                     ggggcgtggCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATCCCTCgcctggg                                   >  2:372422/1‑151 (MQ=255)                                                                                                     ggggcgtggCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATCCCTCgcctgg                                    <  1:350618/150‑1 (MQ=255)                                                                                                          gtggCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGAGGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATCCCTCgcctgggcct                                <  1:35652/149‑1 (MQ=255)                                                                                                              cacaGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATCCCTCGCCTGGGCCTGGCCaa                          <  1:569028/151‑1 (MQ=255)                                                                                                                 aGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATCCCTCGCCTGGGCCTGGCCAAAt                        >  1:663103/1‑150 (MQ=255)                                                                                                                 aGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATCCCTCGCCTGGGCCTGGCCAAATa                       <  2:504070/151‑1 (MQ=255)                                                                                                                         aTTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGTACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATCCCTCGCCTGGGCCTGGCCAAATAAAAAATc                <  2:284613/150‑1 (MQ=255)                                                                                                                         aTTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATCCCTCGCCTGGGCCTGGCCAAATAAAAAATcc               <  2:124344/151‑1 (MQ=255)                                                                                                                           tGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATCCCTCGCCTGGGCCTGGCCAAATAAAAAATcccc             >  2:103734/1‑151 (MQ=255)                                                                                                                            gCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATCCCTCGCCTGGGCCTGGCCAAATAAAAAATCCCCg            >  1:50202/1‑151 (MQ=255)                                                                                                                            gCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATCCCTCGCCTGGGCCTGGCCAAATAAAAAATCCCCg            >  1:336961/1‑151 (MQ=255)                                                                                                                               aTTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATCCCTCGCCTGGGCCTGGCCAAATAAAAAATCCCCGGa          >  1:230277/1‑150 (MQ=255)                                                                                                                                  ggCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATCCCTCGCCTGGGCCTGGCCAAATAAAAAATCCCCGGAAgg       <  2:582583/150‑1 (MQ=255)                                                                                                                                  ggCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATCCCTCGCCTGGGCCTGGCCAAATAAAAAATCCCCGGAAGGc      <  2:585086/151‑1 (MQ=255)                                                                                                                                   gcgcGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATCCCTCGCCTGGGCCTGGCCAAATAAAAAATCCCCGGAAGGc      >  1:89625/1‑150 (MQ=255)                                                                                                                                      cgAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATCCCTCGCCTGGGCCTGGCCAAATAAAAAATCCCCGGAAGGCaaaa  <  1:741451/151‑1 (MQ=255)                                                                                                                                       gAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATTCCTCGCCTGGGCCTGGCCAAATAAAAAATCCCCGGAAGGCaaaa  >  1:606019/1‑150 (MQ=255)                                                                                                                                           |                                                                                                                                                  GCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAACTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATCCCTCGCCTGGGCCTGGCCAAATAAAAAATCCCCGGAAGGCAAAA  >  NC_000913/1028096‑1028379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑