| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,072,718 | A→G | V49A (GTC→GCC) | rutB ← | ureidoacrylate amidohydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,072,718 | 0 | A | G | 97.8% | 141.2 / ‑2.7 | 45 | coding (146/693 nt) | rutB | ureidoacrylate amidohydrolase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (22/22); minor base . (1/0); total (23/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01 | |||||||||||
GTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAAT‑GTTGGCAATGACCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCGTTTTGCATATCCACCACGATCAGCGCACTTTGCTGCGGATCGAAGGTAATGGCTTCCGGTCGAGCGGTTAAGGTCGTCATC > NC_000913/1072574‑1072864 | gTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAAT‑GTTGGCAATGGCCGGGc < 2:356593/151‑1 (MQ=255) ttttCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTTAAT‑GTTGGCAATTGCCggg > 1:502095/1‑149 (MQ=255) ttCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTTAAT‑GTTGGGAATGGGCggggg > 2:719264/1‑147 (MQ=255) ttCGATTTATGAAAAGTCGGTGAGCCGGGCCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGTTCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAAT‑GTTGGCAATGGCCGGGCGAGTGGTTgag < 1:606387/150‑1 (MQ=255) tCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAAT‑GTTGGCAATGGCCGGGCGAGTGGTTgaga < 2:403458/150‑1 (MQ=255) aTGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAAT‑GTTGGCAATGGCCGGGCGAGTGGTTGAGACATCaaa < 1:412233/150‑1 (MQ=255) tGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGGAT‑GTTGGGAATGGCCGGGCGGGGGGGTGAGACATCaaa > 1:130147/1‑149 (MQ=255) ttCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTTTAT‑TTTTGCAATGGCCCGGCGGGGGGGTGAGGCATCaaa > 1:479110/1‑143 (MQ=255) ttCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAAT‑GTTGGCAATGGCCGGGGGAGGGGGTGAGACATCAAAACCgg > 2:89577/1‑148 (MQ=255) gAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAAT‑GTTGGCAATGGCCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCt < 1:398914/150‑1 (MQ=255) aGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAAT‑GTTGGCAATGGCCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTaa < 2:461889/151‑1 (MQ=255) tCCGCCAGCCTCGACATCCTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAAT‑GTTGGCAATGGCCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAgcc < 1:509887/150‑1 (MQ=255) gCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAAT‑GTTGGCAATGGCCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAgccgcc < 1:34735/150‑1 (MQ=255) ccAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTTAAT‑TTTGGCAAAGGGCCGGGGGGGGGGTGAGACATCAAACCCCGCGAGATCTAAGTAgccgcc > 2:131132/1‑149 (MQ=255) ccAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGATTGAAT‑GTTGGGAAATGGCGGGGGAGAGGTTGTGACAACCAACCCCGCGCGATCTAATTAaccgcc > 2:5815/1‑149 (MQ=255) cAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAAT‑GTTGGGAATGGCCGGGCGGGGGGGTGGGACATCAAACCCGGCGAGATCTAAGTAGCCGCCCgg > 1:187168/1‑151 (MQ=255) cATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAAT‑GTTGGCAATGGCCGGGCGAGGGGGTGAGACATCAAACCCGGCGGGATCTAAGTAGCCGCCTGGCGGGGCa > 2:584521/1‑149 (MQ=255) tACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGTAT‑GTTGGCAATGGCCGGGCGGGGGGGTGAGACATCAAAACCGGCGGGGTCTAAGTAGCCGCCCGGCGTGGCATaa > 1:618816/1‑150 (MQ=255) aCTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAAT‑GTTGGCAATGGCCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGc < 1:751999/151‑1 (MQ=255) cATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTTTAT‑ATTGGCAATGGCCGGGCGAGGGGTTGAGACATCAAACCCGGCGGGATCTAAATAGCCGCCCGGCGTGGCATAAACGtttt > 1:569621/1‑150 (MQ=255) cATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAAT‑GTTGGCAATGGCCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCGtttt < 1:654177/150‑1 (MQ=255) ccATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAAT‑GTTGGCAATGGCCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCGTTTTGCATATcca < 2:711994/151‑1 (MQ=255) aTTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAAT‑GTTGGCAATGGCCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCGTTTTGCATATccacc < 2:578010/151‑1 (MQ=255) aaCCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAAT‑GTTGGCAATGGCCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCGTTTTGCATATCCACCACGATCa < 1:105291/151‑1 (MQ=255) aaCCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAAT‑GTTGGCAAAGGCCGGGCGAGGGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCCGGCGTGGCATAAACGTTTTGCATATTcaccac > 2:155672/1‑146 (MQ=255) ccAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGGATGGTTGGCATTG‑CCGGGCGGGGGGGTGAGACATCAAACCCCGCGGGATCTAAGTAGCCGCCCTGCGTGGCCTAAGCGGTTTTGATATCCACCCCggaca > 1:562582/1‑145 (MQ=255) ccAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAAT‑GTTGGCAATGGCCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCGTTTTGCATATCCACCACGATCAgc < 1:41178/151‑1 (MQ=255) atgatCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTTAAT‑GTTGGCAATGGCCGGGCGGGTGGTTGGGACATCCAACCCGGCGGGATCTAAGTAGCCGCCTGGCGTGGGCTAAGCGTTTTGCATATCCACCACGATCAGCGCAc > 1:137396/1‑151 (MQ=255) atgatCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTTAAT‑GTTGGCAATGGCCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCGTTTTTCATATCCACCACGATCAgcg > 2:611712/1‑148 (MQ=255) gCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAAT‑GTTGGCAATGGCCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCGTTTTGCATATCCACCACGATCAGCGCACTTtgct < 2:101921/150‑1 (MQ=255) aTCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAAT‑GTTGGCAATGGCCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCGTTTTGCATATCCACCACGATCAGCGCACTTTGCTGCg < 1:122605/151‑1 (MQ=255) tCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTTTAA‑GTTTGCACAGGCCGGGCGTGGGGGTGGGACAACAAACCCCGCGAGAATATAGTAGCCGGCTGGCGGGGAATAAAGGGGTTGCATATTCCACACGCTCAGCGCACctt > 1:610376/1‑144 (MQ=255) cccTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAAT‑GTTGGCAATGGCCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCGTTTTGCATATCCACCACGATCAGCGCACTTTGCTGCgg < 1:681969/150‑1 (MQ=255) ccTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAAT‑GGTGGGAATGGGCGGGGGAGGGGTTGGGACATAAAAACCCGCGAGATTTAAGAAGGCGCCCGGGGTGGCATAAGAGTTTTGCATATCCCCCACGATTAGCGCACTTTGCTGCgg > 2:214569/1‑149 (MQ=255) tGCCGCTCGCGCTGCGGTCACGGCGGTTTGAAT‑GTTGGCAATTGCCGGGGGGGGGGGTGGGACCTCAAACCCCGCGGGATCTTAGTAGCCGCCCGGCGGGGCCAAAACGGTTTTCCTATCCCCCCCCAACAACGCCCTTTTCTGCGGGTCg > 1:277731/1‑151 (MQ=255) tGCCGCTCGCGCTGCGGTCACGGCGGTTTGAAT‑GTTGGCAATGGCCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCGTTTTGCATATCCACCACGATCAg < 1:710309/132‑1 (MQ=255) tGCCGCTCGCGCTGCGGTCACGGCGGTTTGAAT‑GTTGGCAATGGCCGGGCGAGTGGGTGAGACATCAAACCCCGCGAGATCTAAGTAGCCGCCCGGCGTGGCATAAACGGTTTTCATATCCACCACGATCa > 2:710383/1‑131 (MQ=255) cGCTCGCGCTGCGGTCACGGCGGTTTGAAT‑GTTGGCAATGGCCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCGTTTTGCATATCCACCACGATCAGCGCACTTTGCTGCGGATCGAAg < 2:594246/151‑1 (MQ=255) cgcgCTGCGGTCACGGCGGTTTGAAT‑GTTGGCAATGGCCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCGTTTTGCATATCCACCACGATCAGCGCACTTTGCTGCGGATCGAAGGt < 2:438251/149‑1 (MQ=255) cTGCGGTCACGGCGGTTTGAAT‑GGTGGCAAAGGCCGGGCGAGGGGGTGAGACATCAAACCCCGGGAGATCTAAGGAGCCGCCCGGCGTGGGCTAAGCGTTTTGCATATCCACCACGACAAGCGCACCTTGCTGCGGGGCGGAGGGAATgg > 1:78037/1‑150 (MQ=255) gAAT‑GTTGGCAATGGCCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCGTTTTGCATATCCACCACGATCAGCGCACTTTGCTGCGGATCGAAGGTAATGGCTTCCGGTCGAGCGGTTa > 2:723294/1‑150 (MQ=255) aaT‑GTTGGCAATGGCCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCGTTTTGCATATCCACCACGATCAGCGCACTTTGCTGCGGATCGAAGGTAATGGCTTCCGGTCGAGCGGTTa > 1:520093/1‑149 (MQ=255) gTTGGCAATGGCCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCGTTTTGCATATCCACCACGATCAGCGCACTTTGCTGCGGATCGAAGGTAATGGCTTCCGGTCGAGCGGTTAAGgtc < 1:453641/151‑1 (MQ=255) cAATGGCCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCGTTTTGCATATCCACCACGATCAGCGCACTTTGCTGCGGATCGAAGGTAATGGCTTCCGGTCGAGCGGTTAAGGTCGtcat < 2:499379/151‑1 (MQ=255) aaTGGCCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCGTTTTGCATATCCACCACGATCAGCGCACTTTGCTGCGGATCGAAGGTAATGGCTTCCGGTCGAGCGGTTAAGGTCGtcatc > 1:166026/1‑151 (MQ=255) | GTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAAT‑GTTGGCAATGACCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCGTTTTGCATATCCACCACGATCAGCGCACTTTGCTGCGGATCGAAGGTAATGGCTTCCGGTCGAGCGGTTAAGGTCGTCATC > NC_000913/1072574‑1072864 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |