Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,099,158	G→A	D94N (GAT→AAT)	ycdX →	alkaline phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,099,158	0	G	A	100.0%	140.4 / NA	37	D94N (GAT→AAT)	ycdX	alkaline phosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/14);  total (23/14)

AAGATGCGCCGCATCACTGGCACTTCATTAACATGCGTATCTGGCCGCGAGTGGTTGATGGGGTAGGGATCCTGCGCGGCATCGAAGCTAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGGATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAA  >  NC_000913/1099012‑1099304                                                                                                                                                   |                                                                                                                                                   aaGATGCGCCGCATCACTGGCACTTCATTAACATGCGTATCTGGCCGCGAGTGGTTGATGGGGTAGGGATCCTGCGCGGCATCGAAGCTAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCt                                                                                                                                                <  2:360288/151‑1 (MQ=255)  aGATGCGCCGCATCACTGGCACTTCATTAACATGCGTATCTGGCCGCGAGTGGTTGATGGGGTAGGGATCCTGCGCGGCATCGAAGCTAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCt                                                                                                                                                >  1:46219/1‑150 (MQ=255)        gccgcATCACTGGCACTTCATTAACATGCGTATCTGGCCGCGAGTGGTTGATGGGGTAGGGATCCTGCGCGGCATCGAAGCTAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAattatt                                                                                                                                         >  2:749014/1‑151 (MQ=255)             aTCACTGGCACTTCATTAACATGCGTATCTGGCCGCGAGTGGTTGATGGGGTAGGGATCCTGCGCGGCATCGAAGCTAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCgg                                                                                                                                    >  2:422716/1‑151 (MQ=255)             aTCACTGGCACTTCATTAACATGCGTATCTGGCCGCGAGTGGTTGATGGGGTAGGGATCCTGCGCGGCATCGAAGCTAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCgg                                                                                                                                    >  1:20089/1‑151 (MQ=255)                 cTGGCACTTCATTAACATGCGTATCTGGCCGCGAGTGGTTGATGGGGTAGGGATCCTGCGCGGCATCGAAGCTAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGtttt                                                                                                                                >  2:723357/1‑151 (MQ=255)                     cACTTCATTAACATGCGTATCTGGCCGCGAGTGGTTGATGGGGTAGGGATCCTGCGCGGCATCGAAGCTAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATg                                                                                                                            <  1:256471/151‑1 (MQ=255)                              aaCATGCGTATCTGGCCGCGAGTGGTTGATGGGGTAGGGATCCTGCGCGGCATCGAAGCTAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGttt                                                                                                                   >  1:665375/1‑151 (MQ=255)                                             ccGCGAGTGGTTGATGGGGTAGGGATCCTGCGCGGCATCGAAGCTAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACa                                                                                                    <  2:16127/151‑1 (MQ=255)                                               gcgAGTGGTTGATGGGGTAGGGATCCTGCGCGGCATCGAAGCTAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACaa                                                                                                   >  2:414585/1‑150 (MQ=255)                                                     ggTTGATGGGGTAGGGATCCTGCGCGGCATCGAAGCTAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGAc                                                                                             <  2:257938/150‑1 (MQ=255)                                                             gggTAGGGATCCTGCGCGGCATCGAAGCTAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATAcaca                                                                                    <  1:596611/151‑1 (MQ=255)                                                               gTAGGGATCCTGCGCGGCATCGAAGCTAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATAcaca                                                                                    >  2:397997/1‑149 (MQ=255)                                                               gTAGGGATCCTGCGCGGCATCGAAGCTAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAg                                                                                  <  1:719407/151‑1 (MQ=255)                                                                           cgcgGCATCGAAGCTAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCa                                                                      <  1:434423/151‑1 (MQ=255)                                                                              ggCATCGAAGCTAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACt                                                                    >  2:63243/1‑150 (MQ=255)                                                                              ggCATCGAAGCTAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATAACAAAGCGACCAATACACAAGCGATGATCGCCACt                                                                    >  2:468598/1‑150 (MQ=255)                                                                                 aTCGAAGCTAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACTATCg                                                                >  1:200854/1‑151 (MQ=255)                                                                                   cGAAGCTAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACTATCGcc                                                              <  1:284849/151‑1 (MQ=255)                                                                                         tAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACTATCGCCAGCGGc                                                        >  2:552217/1‑151 (MQ=255)                                                                                         tAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACTATCGCCAGCGGc                                                        >  1:196292/1‑151 (MQ=255)                                                                                         tAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACTATCGCCAGCGGc                                                        >  1:169590/1‑151 (MQ=255)                                                                                          aaCATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACTATCGCCAGCgg                                                         <  2:20118/149‑1 (MQ=255)                                                                                          aaCATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACTATCGCCAGCgg                                                         <  1:228008/149‑1 (MQ=255)                                                                                                  aaaTGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACTATCGCCAGCGGCAATGTGCat                                               >  2:548870/1‑151 (MQ=255)                                                                                                      gTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACTATCGCCAGCGGCAATGTGCAtata                                            <  2:304348/150‑1 (MQ=255)                                                                                                          aTGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACTATCGCCAGCGGCAATGTGCATATAATAAg                                       <  2:245948/151‑1 (MQ=255)                                                                                                              tGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACTATCGCCAGCGGCAATGTGCATATAATAAGCCAt                                   >  1:579158/1‑151 (MQ=255)                                                                                                                 aaTTTACTGCAGCGGTCCAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTGCATGAGCCGGTTTTTGCGACACATGACAAAGCGACCAATACACATGAGATGACCACCCCTATAGCCAAAGGCAAAGTGCATATAATAAGCCATccc                                >  1:467908/1‑151 (MQ=255)                                                                                                                  aTTGACTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCg                               >  1:761162/1‑151 (MQ=255)                                                                                                                       cTGCAGCGGTAAAATGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAAt                          >  1:115906/1‑151 (MQ=255)                                                                                                                                   aaTGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGaa              <  2:196325/151‑1 (MQ=255)                                                                                                                                    aTGTTTGACTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGa               >  2:634285/1‑149 (MQ=255)                                                                                                                                           aCTCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGAtgt      >  1:105387/1‑151 (MQ=255)                                                                                                                                             tCGCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGAtgtg     >  2:429731/1‑150 (MQ=255)                                                                                                                                             ccGCTGAATCTACTTATTGCCGGTTTTCATGAGCCGGTGTTTGCGCCACATGACAAATCGACCAATACACAAGCGATGATCGCCACTATCGCCAGCGGCAAAGTGCATATAATAAACCATCCCGGAAATCCCAAATATGAAATAGAtgt      >  1:539546/2‑149 (MQ=255)                                                                                                                                               gCTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACTATCGCCAGCGGCAATGTGCATTTAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGaaa  <  1:460341/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   AAGATGCGCCGCATCACTGGCACTTCATTAACATGCGTATCTGGCCGCGAGTGGTTGATGGGGTAGGGATCCTGCGCGGCATCGAAGCTAACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGGATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAA  >  NC_000913/1099012‑1099304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑