Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	112,720	G→A	A167V (GCC→GTC)	coaE ←	dephospho‑CoA kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	112,720	0	G	A	100.0%	153.5 / NA	41	A167V (GCC→GTC)	coaE	dephospho‑CoA kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/16);  total (25/16)

TGTTCAAAAAGGACCTGGGTCTGCATTACGGTTTTTCCTGTGAGACAAACTGCGACGCAAGCTGCAAATAGTGTGCGTGCAGGCGGGCAACATCCGATGCGATAGCATCCGGTGCGCCGTTATTATCAATGACGTCATCTGCCACGGCAAGGCGGGCTTCGCGCGTTGCCTGAGCAGCAAGGATTTGTTCGACATGCTCGCGAGTTACATCATCGCGCTGCATGGTGCGCTTAAGTTGCGTTTCTGGGCTGACATCCACCACAAGCACTCGATTCGCTTTTTTATACAGTGAGT  >  NC_000913/112574‑112867                                                                                                                                                   |                                                                                                                                                    tGTTCAAAAAGGACCTGGGTCTGCATTACGGTTTTTCCTGTGAGACAAACTGCGACGCAAGCTGCAAATAGTGTGCGTGCAGGCGGGCAACATCCGATGCGATAGCATCCGGTGCGCCGTTATTATCAATGACGTCATCTGCCACGACa                                                                                                                                                   >  2:86807/1‑149 (MQ=255)   ttCAAAAAGGACCTGGGTCTGCATTACGGTTTTTCCTGTGAGACAAACTGCGACGCAAGCTGCAAATAGTGTGCGTGCAGGCGGGCAACATCCGATGCGATAGCATCCGGTGCGCCGTTATTATCAATGACGTCATCTGCCACGACAAgg                                                                                                                                                >  1:446133/1‑150 (MQ=255)    tCAAAAAGGACCTGGGTCTGCATTACGGTTTTTCCTGTGAGACAAACTGCGACGCAAGCTGCAAATAGTGTGCGTGCAGGCGGGCAACATCCGATGCGATAGCATCCGGTGCGCCGTTATTATCAATGACGTCATCTGCCACGACAAGGc                                                                                                                                               <  1:71565/150‑1 (MQ=255)      aaaaaGGACCTGGGTCTGCATTACGGTTTTTCCTGTGAGACAAACTGCGACGCAAGCTGCAAATAGTGTGCGTGCAGGCGGGCAACATCCGATGCGATAGCATCCGGTGCGCCGTTATTATCAATGACGTCATCTGCCACGACAAGGCggg                                                                                                                                            >  2:204174/1‑151 (MQ=255)       aaaaGGACCTGGGTCTGCATTACGGTTTTTCCTGTGAGACAAACTGCGACGCAAGCTGCAAATAGTGTGCGTGCAGGCGGGCAACATCCGATGCGATAGCATCCGGTGCGCCGTTATTATCAATGACGTCATCTGCCACGACAAGGCgg                                                                                                                                             >  1:544767/1‑149 (MQ=255)           ggACCTGGGTCTGCATTACGGTTTTTCCTGTGAGACAAACTGCGACGCAAGCTGCAAATAGTGTGCGTGCAGGCGGGCAACATCCGATGCGATAGCATCCGGTGCGCCGTTATTATCAATGACGTCATCTGCCACGACAAGGCGGGCTTcg                                                                                                                                       <  2:136755/151‑1 (MQ=255)               cTGGGTCTGCATTACGGTTTTTCCTGTGAGACAAACTGCGACGCAAGCTGCAAATAGTGTGCGTGCAGGCGGGCAACATCCGATGCGATAGCATCCGGTGCGCCGTTATTATCAATGACGTCATCTGCCACGACAAGGCGGGCTTcgcgcg                                                                                                                                   >  2:141371/1‑151 (MQ=255)               cTGGGTCTGCATTACGGTTTTTCCTGTGAGACAAACTGCGACGCAAGCTGCAAATAGTGTGCGTGCAGGCGGGCAACATCCGATGCGATAGCATCCGGTGCGCCGTTATTATCAATGACGTCATCTGCCACGACAAGGCGGGCTTcgcgcg                                                                                                                                   >  2:467175/1‑151 (MQ=255)               cTGGGTCTGCATTACGGTTTTTCCTGTGAGACAAACTGCGACGCAAGCTGCAAATAGTGTGCGTGCAGGCGGGCAACATCCGATGCGATAGCATCCGGTGCGCCGTTATTATCAATGACGTCATCTGCCACGACAAGGCGGGCTTcgcgcg                                                                                                                                   >  2:741255/1‑151 (MQ=255)                tGGGTCTGCATTACGGTTTTTCCTGTGAGACAAACTGCGACGCAAGCTGCAAATAGTGTGCGTGCAGGCGGGCAACATCCGATGCGATAGCATCCGGTGCGCCGTTATTATCAATGACGTCATCTGCCACGACAAGGCGGGCTTcgcgcg                                                                                                                                   >  1:706143/1‑150 (MQ=255)                 gggTCTGCATTACGGTTTTTCCTGTGAGACAAACTGCGACGCAAGCTGCAAATAGTGTGCGTGCAGGCGGGCAACATCCGATGCGATAGCATCCGGTGCGCCGTTATTATCAATGACGTCATCTGCCACGACAAGGCGGGCTTCGCGCGtt                                                                                                                                 >  2:59568/1‑151 (MQ=255)                 gggTCTGCATTACGGTTTTTCCTGTGAGACAAACTGCGACGCAAGCTGCAAATAGTGTGCGTGCAGGCGGGCAACATCCGATGCGATAGCATCCGGTGCGCCGTTATTATCAATGACGTCATCTGCCACGACAAGGCGGGCTTCGCGCGtt                                                                                                                                 <  1:647110/151‑1 (MQ=255)                 gggTCTGCATTACGGTTTTTCCTGTGAGACAAACTGCGACGCAAGCTGCAAATAGTGTGCGTGCAGGCGGGCAACATCCGATGCGATAGCATCCGGTGCGCCGTTATTATCAATGACGTCATCTGCCACGACAAGGCGGGCTTCGCGCGtt                                                                                                                                 <  1:134355/151‑1 (MQ=255)                  ggTCTGCATTACGGTTTTTCCTGTGAGACAAACTGCGACGCAAGCTGCAAATAGTGTGCGTGCAGGCGGGCAACATCCGATGCGATAGCATCCGGTGCGCCGTTATTATCAATGACGTCATCTGCCACGACAAGGCGGGCTTCGCGCGTTg                                                                                                                                >  2:59105/1‑151 (MQ=255)                      tGCATTACGGTTTTTCCTGTGAGACAAACTGCGACGCAAGCTGCAAATAGTGTGCGTGCAGGCGGGCAACATCCGATGCGATAGCATCCGGTGCGCCGTTATTATCAATGACGTCATCTGCCACGACAAGGCGGGCTTCGCGCGTTGCCTg                                                                                                                            >  2:355574/1‑151 (MQ=255)                                      cTGTGAGACAAACTGCGACGCAAGCTGCAAATAGTGTGCGTGCAGGCGGGCAACATCCGATGCGATAGCATCCGGTGCGCCGTTATTATCAATGACGTCATCTGCCACGACAAGGCGGGCTTCGCGCGTTGCCTGAGCAGCAAGGATTTGt                                                                                                            >  1:200193/1‑151 (MQ=255)                                       tgtgAGACAAACTGCGACGCAAGCTGCAAATAGTGTGCGTGCAGGCGGGCAACATCCGATGCGATAGCATCCGGTGCGCCGTTATTATCAATGACGTCATCTGCCACGACAAGGCGGGCTTCGCGCGTTGCCTGAGCAGCAAGGATTTGtt                                                                                                           >  2:337440/1‑151 (MQ=255)                                       tgtgAGACAAACTGCGACGCAAGCTGCAAATAGTGTGCGTGCAGGCGGGCAACAGCCGATGCGATAGCATCCGGTGCGCCGTTATTATCAATGACGTCATCTGCCACGACAAGGCGGGCTTCGCGCGTTGCCTGAGCAGCAAGGAtt                                                                                                               <  1:104200/147‑1 (MQ=255)                                             aCAAACTGCGACGCAAGCTGCAAATAGTGTGCGTGCAGGCGGGCAACATCCGATGCGATAGCATCCGGTGCGCCGTTATTATCAATGACGTCATCTGCCACGACAAGGCGGGCTTCGCGCGTTGCCTGAGCAGCAAGGATTTGTTCGa                                                                                                        >  1:342170/1‑148 (MQ=255)                                                aaCTGCGACGCAAGCTGCAAATAGTGTGCGTGCAGGCGGGCAACATCCGATGCGATAGCATCCGGTGCGCCGTTATTATCAATGACGTCATCTGCCACGACAAGGCGGGCTTCGCGCGTTGCCTGAGCAGCAAGGATTTGTTCGACATGcg                                                                                                  <  1:709570/151‑2 (MQ=255)                                                     cGACGCAAGCTGCAAATAGTGTGCGTGCAGGCGGGCAACATTCGATGCGATAGCCTCCGGTGCGCCGTTATTATCAATGACGTCATCTGCCACGACAAGGCGGGCCTCGCCCGGTGCCTGAGCAGCCAGGGTTTTTTCGACATGCCcgcg                                                                                              >  1:120374/1‑150 (MQ=255)                                                     cGACGCAAGCTGCAAATAGTGTGCGTGCAGGCGGGCAACATCCGATGCGATAGCATCCGGTGCGCCGTTATTATCAATGACGTCATCTGCCACGACAAGGCGGGCCTCCCGCGGTGGCTGAGCAGCAAGGATTTGTTCGACATGCTcgc                                                                                               >  2:263432/1‑149 (MQ=255)                                                        cGCAAGCTGCAAATAGTGTGCGTGCAGGCGGGCAACATCCGATGCGATAGCATCCGGTGCGCCGTTATTATCAATGACGTCATCTGCCACGACAAGGCGGGCTTCGCGCGTTGCCTGAGCAGCAAGGATTTGTTCGACATGCTCGCGAGt                                                                                           <  2:449084/150‑1 (MQ=255)                                                          cAAGCTGCAAATAGTGTGCGTGCAGGCGGGCAACATCCGATGCGATAGCATCCGGTGCGCCGTTATTATCAATGACGTCATCTGCCACGACAAGGCGGGCTTCGCGCGTTGCCTGAGCAGCCAGGATTTGTTCGACATGCTCGCGAGTTa                                                                                         >  1:449206/1‑150 (MQ=255)                                                            aGCTGCAAATAGTGTGCGTGCAGGCGGGCAACATCCGATGCGATAGCATCCGGTGCGCCGTTATTATCAATGACGTCATCTGCCACGACAAGGCGGGCTTCGCGCGTTGCCTGAGCAGCAAGGATTTGTTCGACATGCTCGCGAGTTAcat                                                                                      <  1:289198/151‑1 (MQ=255)                                                                  aaaTAGTGTGCGTGCAGGCGGGCAACATCCGATGCGATAGCATCCGGTGCGCCGTTATTATCAATGACGTCATCTGCCACGACAAGGCGGGCTTCGCGCGTTGCCTGAGCAGCAAGGATTTGTTCGACATGCTCGCGAGTTACATCATcgc                                                                                >  2:259188/1‑151 (MQ=255)                                                                  aaaTAGTGTGCGTGCAGGCGGGCAACATCCGATGCGATAGCATACGGTGCGCTGTTATTATCAATGACGTCATCTGCCACGACAAGGCGGGGTTCGCGCGTTGCCTGAGCAGCAAGGATTTGTTCGACATGCCCGCGAGGTACATCATcg                                                                                 >  1:301761/1‑150 (MQ=255)                                                                            cgtgcAGGCGGGCAACATCCGATGCGATAGCATCCGGTGCGCCGTTATTATCAATGACGTCATCTGCCACGACAAGGCGGGCTTCGCGCGTTGCCTGAGCAGCAAGGATTTGTTCGACATGCTCGCGAGTTACATCATCGCGCTGCATGGt                                                                      <  2:246019/151‑1 (MQ=255)                                                                                  ggCGGGCAACATCCGATGCGATAGCATCCGGTGCGCCGTTATTATCAATGACGTCATCTGCCACGACAAGGCGGGCTTCGCGCGTTGCCTGAGCAGCAAGGATTTGTTCGACATGCTCGCGAGTTACATCATCGCGCTGCATGGTGCGCtt                                                                <  2:538532/151‑1 (MQ=255)                                                                                   gCGGGCAACATCCGATGCGATAGCATCCGGTGCGCCGTTATTATCAATGACGTCATCTGCCACGACAAGGCGGGCTTCGCGCGTTGCCTGAGCAGCAAGGATTTGTTCGACATGCTCGCGAGTTACATCATCGCGCTGCATGGTGCGCTTa                                                               <  1:152605/151‑1 (MQ=255)                                                                                       gCAACATCCGATGCGATAGCATCCGGTGCGCCGTTATTATCAATGACGTCATCTGCCACGACAAGGCGGGCTTCGCGCGTTGCCTGAGCAGCAAGGATTTGTTCGACATGCTCGCGAGTTACATCATCGCGCTGCATGGTGCGCTTAAGtt                                                           <  2:178000/151‑1 (MQ=255)                                                                                        cAACATCCGATGCGATAGCATCCGGTGCGCCGTTATTATCAATGACGTCATCTGCCACGACAAGGCGGGCTTCGCGCGTTGCCTGAGCAGCAAGGATTTGTTCGACATGCTCGCGAGTTACATCATCGCGCTGCATGGTGCGCTTAAGTTg                                                          <  2:406262/151‑1 (MQ=255)                                                                                         aaCATCCGATGCGATAGCATCCGGTGCGCCGTTATTATCAATGACGTCATCTGCCACGACAAGGCGGGCTTCGCGCGTTGCCTGAGCAGCAAGGATTTGTTCGACATGCTCGCGAGTTACATCATCGCGCTGCATGGTGCGCTTAAGTTGc                                                         >  1:736165/1‑151 (MQ=255)                                                                                              ccGATGCGATAGCATCCGGTGCGCCGTTATTATCAATGACGTCATCTGCCACGACAAGGCGGGCTTCGCGCGTTGCCTGAGCAGCAAGGATTTGTTCGACATGCTCGCGAGTTACATCATCGCGCTGCATGGTGCGCTTAAGTTGCGttt                                                     <  2:25199/150‑1 (MQ=255)                                                                                               cGATGCGATAGCATCCGGTGCGCCGTTATTATCAATGACGTCATCTGCCACGACAAGGCGGGCTTCGCGCGTTGCCTGAGCAGCAAGGATTTGTTCGACATGCTCGCGAGTTACATCATCGCGCTGCATGGTGCGCTTAAGTTGCGTTTCt                                                   >  2:681302/1‑151 (MQ=255)                                                                                                  tGCGATAGCATCCGGTGCGCCGTTATTATCAATGACGTCATCTGCCACGACAAGGCGGGCTTCGCGCGTTGCCTGAGCAGCAAGGATTTGTTCGACATGCTCGCGAGTTACATCATCGCGCTGCATGGTGCGCTTAAGTTGCGTTTCTggg                                                >  2:269804/1‑151 (MQ=255)                                                                                                        aGCATCCGGTGCGCCGTTATTATCAATGACGTCATCTGCCACGACAAGGCGGGCTTCGCGCGTTGCCTGAGCAGCAAGGATTTGTTCGACATGCTCGCGAGTTACATCATCGCGCTGCATGGTGCGCTTAAGTTGCGTTTCTGGGCTGAc                                           >  1:722031/1‑150 (MQ=255)                                                                                                         gCATCCGGTGCGCCGTTATTATCAATGACGTCATCTGCCACGACAAGGCGGGCTTCGCGCGTTGCCTGAGCAGCAAGGATTTGTTCGACATGCTCGCGAGTTACATCATCGCGCTGCATGGTGCGCTTAAGTTGCGTTTCTGGGCTGACAt                                         <  2:90588/151‑1 (MQ=255)                                                                                                                                   gACGTCATCTGCCACGACAAGGCGGGCTTCGCGCGTTGCCTGAGCAGCAAGGATTTGTTCGACATGCTCGCGAGTTACATCATCGCGCTGCATGGTGCGCTTAAGTTGCGTTTCTGGGCTGACATCCACCACAAGCACTCGATTCGCttt                <  1:595932/150‑1 (MQ=255)                                                                                                                                       tCATCTGCCACGACAAGGCGGGCCTCGCGCGTTGCCTGAGCAGCAAGGATTTGTTCGACATGCTCGCGAGTTACATCATCGCGCTGCATGGTGCGCTTAAGTTGCGTTTCTGGGCTGACATCCACCACAAGCACTCGATTCGCTTTTTtat           >  2:111794/1‑151 (MQ=255)                                                                                                                                                aCGACAAGGCGGGCTTCGCGCGTTGCCTGAGCAGCAAGGATTTGTTCGACATGCTCGCGAGTTACATCATCGCGCTGCATGGTGCGCTTAAGTTGCGTTTCTGGGCTGACATCCACCACAAGCACTCGATTCGCTTTTTTATACAGTGAGt  >  2:510509/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                                    TGTTCAAAAAGGACCTGGGTCTGCATTACGGTTTTTCCTGTGAGACAAACTGCGACGCAAGCTGCAAATAGTGTGCGTGCAGGCGGGCAACATCCGATGCGATAGCATCCGGTGCGCCGTTATTATCAATGACGTCATCTGCCACGGCAAGGCGGGCTTCGCGCGTTGCCTGAGCAGCAAGGATTTGTTCGACATGCTCGCGAGTTACATCATCGCGCTGCATGGTGCGCTTAAGTTGCGTTTCTGGGCTGACATCCACCACAAGCACTCGATTCGCTTTTTTATACAGTGAGT  >  NC_000913/112574‑112867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑