Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,119,351	C→T	G33R (GGA→AGA)	yceO ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,119,351	0	C	T	100.0%	164.0 / NA	43	G33R (GGA→AGA)	yceO	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/17);  total (26/17)

GAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGT  >  NC_000913/1119203‑1119497                                                                                                                                                     |                                                                                                                                                   gagaTGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATctcc                                                                                                                                                  >  1:271831/1‑151 (MQ=255)      gTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCagag                                                                                                                                              <  2:528404/150‑1 (MQ=255)          ggTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGaaa                                                                                                                                           <  1:486918/149‑1 (MQ=255)           gTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAg                                                                                                                                        <  1:302437/151‑1 (MQ=255)           gTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAg                                                                                                                                        <  2:365722/151‑1 (MQ=255)                 gCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATaaa                                                                                                                                   <  2:7502/150‑1 (MQ=255)                 gCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATaaa                                                                                                                                   <  2:650459/150‑1 (MQ=255)                    gTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCAt                                                                                                                               >  1:545690/1‑151 (MQ=255)                    gTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCAt                                                                                                                               <  2:678694/151‑1 (MQ=255)                     tAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATc                                                                                                                              >  2:603258/1‑151 (MQ=255)                           caagAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGc                                                                                                                        >  1:324067/4‑151 (MQ=255)                               aataatCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTcgc                                                                                                                    >  1:459831/1‑151 (MQ=255)                                    tctcATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAat                                                                                                                >  2:775757/1‑150 (MQ=255)                                       cATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAAtatt                                                                                                             <  2:157797/150‑1 (MQ=255)                                        aTTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAAtatta                                                                                                            <  2:543523/150‑1 (MQ=255)                                          tCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATtg                                                                                                         >  1:44602/1‑151 (MQ=255)                                               ttttCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATtgatg                                                                                                      >  2:156560/1‑149 (MQ=255)                                                       cTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGc                                                                                             >  1:461465/1‑150 (MQ=255)                                                             gAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGcc                                                                                      <  1:507547/151‑1 (MQ=255)                                                             gAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGcc                                                                                      <  1:775681/151‑1 (MQ=255)                                                              aaaGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCg                                                                                     >  2:96160/1‑151 (MQ=255)                                                                  ggAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGc                                                                                 >  2:350411/1‑151 (MQ=255)                                                                    agaTTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCAt                                                                               >  1:375227/1‑151 (MQ=255)                                                                        tAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTaa                                                                            >  2:16396/1‑150 (MQ=255)                                                                         aaaTATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGc                                                                                             >  2:70533/1‑132 (MQ=255)                                                                         aaaTATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGc                                                                                             <  1:70502/132‑1 (MQ=255)                                                                                tGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTg                                                                   >  2:206732/1‑151 (MQ=255)                                                                                tGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACCCCTg                                                                   >  1:632110/1‑151 (MQ=255)                                                                                     gTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTaaa                                                               >  2:671645/1‑150 (MQ=255)                                                                                      tttATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTaaaa                                                              >  1:305965/1‑150 (MQ=255)                                                                                         aTGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACgg                                                          >  2:366650/1‑151 (MQ=255)                                                                                             cAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTc                                                      <  2:332377/151‑1 (MQ=255)                                                                                              aGTGCGGAAAACTAAAAATAAAACCCATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCa                                                     <  2:250651/151‑1 (MQ=255)                                                                                                    gAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGCTAAAGCATCAGATACTCGCGACTATTATTGCTGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTg                                                >  2:570158/1‑150 (MQ=255)                                                                                                       aaCTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAgg                                            >  2:43575/1‑151 (MQ=255)                                                                                                        aCTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGc                                           >  1:464956/1‑151 (MQ=255)                                                                                                                            atcagatcCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGa                        >  2:500876/1‑150 (MQ=255)                                                                                                                                 atcCATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCggg                  <  2:346555/151‑1 (MQ=255)                                                                                                                                   ccATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGa                 >  2:68083/1‑150 (MQ=255)                                                                                                                                    cATAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGaa                >  1:584285/1‑150 (MQ=255)                                                                                                                                     aTAATTGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGaa                >  2:653529/1‑149 (MQ=255)                                                                                                                                         ttGCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTg          >  2:57884/1‑151 (MQ=255)                                                                                                                                           gCCAACAATCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGa         <  1:79080/150‑1 (MQ=255)                                                                                                                                                 aaTCTCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGt  <  2:442891/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   GAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGT  >  NC_000913/1119203‑1119497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑