Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,148,867	A→G	T37A (ACC→GCC)	fabH →	3‑oxoacyl‑[acyl‑carrier‑protein] synthase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,148,867	0	A	G	100.0%	126.4 / NA	35	T37A (ACC→GCC)	fabH	3‑oxoacyl‑[acyl‑carrier‑protein] synthase III
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/17);  total (18/17)

GCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACCAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACG  >  NC_000913/1148720‑1149014                                                                                                                                                    |                                                                                                                                                    gCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTGCCg                                                                                                                                                  >  2:552869/1‑151 (MQ=255)  cAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTGCCgg                                                                                                                                                 <  2:677205/151‑1 (MQ=255)    aGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTGCCGGTa                                                                                                                                               <  1:181365/151‑1 (MQ=255)     gTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTGCCGGTAt                                                                                                                                              >  1:282926/1‑151 (MQ=255)               ataACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTGCCGGTATCCGCGAACg                                                                                                                                     <  2:71060/150‑1 (MQ=255)                             aCTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTGCCGGTATCCGCGAACGCCACATTGCCGCGcc                                                                                                                      >  2:220931/1‑151 (MQ=255)                                gAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTGCCGGTATCCGCGAACGCCACATTGCCGCGCCaaa                                                                                                                   <  1:203323/151‑1 (MQ=255)                                gAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTGCCGGTATCCGCGAACGCCACATTGCCGCGCCaaa                                                                                                                   <  1:455252/151‑1 (MQ=255)                                    gTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTGCCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGa                                                                                                                >  2:713467/1‑150 (MQ=255)                                    gTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTGCAGGTATCCGCGAACGCCACATTGCCGCGCCAAACGaa                                                                                                               <  2:677116/151‑1 (MQ=255)                                     tACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTGCCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGaa                                                                                                               >  2:626381/1‑150 (MQ=255)                                       cATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTGCCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAAcc                                                                                                            <  2:331284/151‑1 (MQ=255)                                                 aaGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTGCCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCa                                                                                                  <  2:269794/151‑1 (MQ=255)                                                       attGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTGCCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCt                                                                                            <  2:93291/151‑1 (MQ=255)                                                       attGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTGCCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCt                                                                                            <  2:41386/151‑1 (MQ=255)                                                                 gCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTGCCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAgcggc                                                                                  <  2:157585/151‑1 (MQ=255)                                                                    gCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTGCCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGac                                                                               <  1:453096/151‑1 (MQ=255)                                                                      tATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTGCCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGacac                                                                             >  1:339184/1‑151 (MQ=255)                                                                           gCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTGCCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACAcgcg                                                                          <  1:510577/149‑1 (MQ=255)                                                                             ccGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTGCCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAAt                                                                      >  1:332586/1‑151 (MQ=255)                                                                             ccGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTGCCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAAt                                                                      >  1:735137/1‑151 (MQ=255)                                                                                   aaGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTGCCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGAt                                                                <  1:391668/151‑1 (MQ=255)                                                                                        cGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTGCCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCgg                                                           >  1:87406/1‑151 (MQ=255)                                                                                            cAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTGCCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCa                                                        <  1:586546/150‑1 (MQ=255)                                                                                                       ttGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTGCCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGAcc                                            >  2:233452/1‑151 (MQ=255)                                                                                                             aaaaTGGTGGACACCTCTGACGAGTGGATTGTCACTCGTGCCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACCAGATTg                                      >  2:38944/1‑151 (MQ=255)                                                                                                              aaaTGGTGGACACCTCTGACGAGTGGATTGTCACTCGTGCCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACCAGATTgg                                     >  1:311456/1‑151 (MQ=255)                                                                                                               aaTGGTGGACACCTCTGACGAGTGGATTGTCACTCGTGCCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACCAGATTGGc                                    >  2:544427/1‑151 (MQ=255)                                                                                                                         acCTCTGACGAGTGGATTGTCACTCGTGCCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACCAGATTGGCCTGATCGTtg                          >  1:703255/1‑151 (MQ=255)                                                                                                                           ctctGACGAGTGGATTGTCACTCGTGCCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACCAGATTGGCCTGATCGTtgt                         <  1:383764/150‑1 (MQ=255)                                                                                                                               gACGAGTGGATTGTCACTCGTGCCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACCAGATTGGCCTGATCGTTGTgg                       <  2:629991/148‑1 (MQ=255)                                                                                                                                aCGAGTGGATTGTCACTCGTGCCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACCAGATTGGCCTGATCGTTGTGGCAAc                   >  1:23889/1‑151 (MQ=255)                                                                                                                                        aTTGTCACTCGTGCCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACCAGATTGGCCTGATCGTTGTGGCAACGACTTCTg           >  2:388187/1‑151 (MQ=255)                                                                                                                                             cACTCGTGCCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACCAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTacg      >  2:533582/1‑151 (MQ=255)                                                                                                                                                 cGTGCCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACCAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTacgcacg  >  1:527274/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                    GCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACCAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACG  >  NC_000913/1148720‑1149014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑