Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,151,418	A→G	intergenic (+14/‑197)	fabG → / → acpP	3‑oxoacyl‑[acyl‑carrier‑protein] reductase/acyl carrier protein (ACP)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,151,418	0	A	G	100.0%	157.0 / NA	43	intergenic (+14/‑197)	fabG/acpP	3‑oxoacyl‑[acyl‑carrier‑protein] reductase/acyl carrier protein (ACP)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/24);  total (19/24)

GCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGTGGTAAGACCTGCCGGGATTTAGTTGCAAATTTTTCAACATTTTATA  >  NC_000913/1151275‑1151565                                                                                                                                                |                                                                                                                                                    gcAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCt                                                                                                                                              <  2:243015/151‑1 (MQ=255)  cAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTc                                                                                                                                             <  2:435551/151‑1 (MQ=255)      ttCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATg                                                                                                                                          >  2:725055/1‑150 (MQ=255)           gCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTgc                                                                                                                                      <  1:325994/149‑1 (MQ=255)               atCGCCACGACGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACAAGGTCTGACCCGGATTTGCACGAAATGCTCATGTTgcg                                                                                                                                     >  1:374833/2‑146 (MQ=255)                   ccTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTg                                                                                                                            >  2:693509/1‑151 (MQ=255)                         cggcgCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGt                                                                                                                      >  2:11483/1‑151 (MQ=255)                            cgcACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTAt                                                                                                                   <  2:242070/151‑1 (MQ=255)                             gcACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATg                                                                                                                  <  2:46097/151‑1 (MQ=255)                             gcACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATg                                                                                                                  <  1:176171/151‑1 (MQ=255)                                 aGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCTCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGta                                                                                                              <  1:747853/151‑1 (MQ=255)                                  ggAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGtaa                                                                                                             <  1:243063/151‑1 (MQ=255)                                  ggAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGtaa                                                                                                             <  2:585234/151‑1 (MQ=255)                                    aaaTCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGtaata                                                                                                           <  1:348965/151‑1 (MQ=255)                                    aaaTCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGtaat                                                                                                            >  1:595936/1‑150 (MQ=255)                                         gCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAAtt                                                                                                        >  2:567890/1‑149 (MQ=255)                                            aaCGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTg                                                                                                    >  1:294008/1‑150 (MQ=255)                                                     gCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGAt                                                                                           <  2:294819/150‑1 (MQ=255)                                                     gCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGAt                                                                                           <  1:481114/150‑1 (MQ=255)                                                     gCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGAt                                                                                           <  1:675334/150‑1 (MQ=255)                                                           cTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGGGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGtt                                                                                    >  1:323744/1‑151 (MQ=255)                                                               cATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTg                                                                                <  2:282748/151‑1 (MQ=255)                                                                aTCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTgg                                                                               <  1:17664/151‑1 (MQ=255)                                                                aTCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTgg                                                                               <  2:25853/151‑1 (MQ=255)                                                                   cgacgaAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTggggg                                                                            <  2:112813/151‑1 (MQ=255)                                                                    gacgaAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTgggggg                                                                           >  2:306403/1‑151 (MQ=255)                                                                           cagcTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGcc                                                                    <  2:706552/151‑1 (MQ=255)                                                                            agcTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCt                                                                   <  2:357128/151‑1 (MQ=255)                                                                                   aTCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAAt                                                             >  1:37201/1‑150 (MQ=255)                                                                                     cACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAAc                                                          <  2:220410/151‑1 (MQ=255)                                                                                        gggTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTa                                                       <  1:121459/151‑1 (MQ=255)                                                                                           tGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTaaa                                                     >  2:138631/1‑150 (MQ=255)                                                                                              aaCTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCg                                                 >  2:456154/1‑151 (MQ=255)                                                                                               aCTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGt                                                <  2:472837/151‑1 (MQ=255)                                                                                               aCTTTGCATGTGAACGGCGGGATGTACATGGTCTGAACGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCg                                                 >  2:365186/1‑150 (MQ=255)                                                                                                  ttGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGTg                                               <  1:636246/149‑1 (MQ=255)                                                                                                              agcggGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGTGGTAAGACCTGCCgg                                 >  1:191433/2‑151 (MQ=255)                                                                                                                 gggATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGTGGTAAGACCTGCCGGGAt                              >  1:156866/1‑151 (MQ=255)                                                                                                                  ggATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGTGGTAAGACCTGCCGGGAtt                             >  2:269970/1‑151 (MQ=255)                                                                                                                    aTGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGTGGTAAGACCTGCCGGGAttt                            >  1:361228/1‑150 (MQ=255)                                                                                                                                 gACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGTGGTAAGACCTGCCGGGATTTAGTTGCAAAtttt               <  2:189282/150‑1 (MQ=255)                                                                                                                                   ccGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGTGGTAAGACCTGCCGGGATTTAGTTGCAAATTTTTc             >  1:297120/1‑150 (MQ=255)                                                                                                                                   ccGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGTGGTAAGACCTGCCGGGATTTAGTTGCAAATTTTTc             >  1:8480/1‑150 (MQ=255)                                                                                                                                             cacgAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGTGGTAAGACCTGCCGGGATTTAGTTGCAAATTTTTCAACATTTtata  <  1:443201/151‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                    GCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGTGGTAAGACCTGCCGGGATTTAGTTGCAAATTTTTCAACATTTTATA  >  NC_000913/1151275‑1151565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑