Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,174,596	A→G	S190S (AGT→AGC)	ycfT ←	inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,174,596	0	A	G	97.4%	102.0 / NA	39	S190S (AGT→AGC)	ycfT	inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (21/17);  minor base C (0/1);  total (21/18)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGC  >  NC_000913/1174448‑1174738                                                                                                                                                     |                                                                                                                                               agaTAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCt                                                                                                                                              >  2:7422/1‑151 (MQ=255) agaTAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCt                                                                                                                                              >  2:673557/1‑151 (MQ=255)         aacaacCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATTc                                                                                                                                      >  1:301552/1‑151 (MQ=255)                       gaccgCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATTCCCCACCACGGCGTg                                                                                                                        >  2:349231/1‑151 (MQ=255)                                aaCGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATTCCCCACCACGGCGTGGGAACGaa                                                                                                                >  2:235142/1‑150 (MQ=255)                                 aCGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATTCCCCACCACGGCGTGGGAACGAAAt                                                                                                              <  2:501286/151‑1 (MQ=255)                                          aaaGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGcc                                                                                                      >  2:470549/1‑150 (MQ=255)                                                 ccATCAGAAGATGGCGGCGCACCGGCACCTTTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAAAAAGGAAAATTGCTGATCACCCTGTTCATCCCCCCCCCCGGCGGGGAAACAAAATAAACCGCCACACTcag                                                                                              <  1:643758/151‑1 (MQ=255)                                                   aTCAGCAGAGGGCGGCGAAACGGCACCTCTTAAACACAGGTCATTATTTTGGCGCCAAACCATGCGCCAAGGCGTTAAAAAGGAAAATGGCGGATCCCGCTTTTCTTTCCCCACCCCGGCGGGGGAACAAAATTAACCGCCACACTca                                                                                               <  1:369807/148‑1 (MQ=255)                                                    tCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATTCCCCACCACGGCGTGGGCACGAAATTAACCGCCACACTcagc                                                                                             >  1:220660/1‑149 (MQ=255)                                                            tGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTacaaac                                                                                    >  1:374944/1‑150 (MQ=255)                                                                ggcgCCACGGCCCCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCATGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCCGTacaaacaa                                                                                  >  1:517485/1‑148 (MQ=255)                                                                ggcgCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAAAAAGGAAAATGGCGGATCACGCTGTTCATCCCCCACCACGGCGTGGGAACAAAATTAACCGCCACACTCAGCAGTacaaaca                                                                                   <  2:24205/147‑1 (MQ=255)                                                                ggcgCAACGGCACCTCTTAAACACAGGTCATTATTGTGGCGCAAACCCATGCGCCAGGGCTGTAAAAAGGAAAATGGCGGATCACGCTGTTCATCCCCCACCCCGGCGGGGGAACGAAATTAACCGCCACACTCAGCAGTACCAACAAGGc                                                                               <  2:528820/151‑1 (MQ=255)                                                                          cACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGc                                                                     >  1:225431/1‑151 (MQ=255)                                                                           cccTCTTTAACACAGTTAATTATTGTTGCGCAAAACCAGGCGCAAAGGTTGTAATAAGCAAAGTGGCGGACCCCGCTTTCCATCCCCCCCCACGGGGGGGAAAAGAAATAAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCa                                                                    <  1:276344/150‑1 (MQ=255)                                                                            ccTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGc                                                                     >  2:450878/1‑149 (MQ=255)                                                                                ttaaaCACAGTTCATTATTGTTGCGCAAACCCATGCGCAAAGGCTGTAAAAAGGCAAATTGCGGATCCCGCTGTTCATCCCCCCCCCCGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAgc                                                                  <  2:337835/145‑1 (MQ=255)                                                                                   aaCACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAgc                                                                  >  1:24037/1‑145 (MQ=255)                                                                                        aGGTCATTATTGTTGCGCAAAACCATGCGCCAAGGCTGTAATAAGGAAAATTGCGGATCACGCTTTTCATCCCCCCCCACGGCGTGGAAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGc                                                        <  2:154630/150‑1 (MQ=255)                                                                                         ggTCATTTTTGTTGCGCCAACCCATGCGCCAAGGCTGTAAAAAGGAAAATTGCGGATCACGCTTTTCATCCCCCCCCCCGGCGGGGGAACAAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTa                                                      <  1:673499/151‑1 (MQ=255)                                                                                           tCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTaa                                                     >  2:65108/1‑150 (MQ=255)                                                                                            cATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTaaa                                                    >  2:136694/1‑150 (MQ=255)                                                                                             attattGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTaaaa                                                   >  2:269477/1‑150 (MQ=255)                                                                                              ttattGTGGCGCAAAACCAGGCGCCAAGGCTGAAATAAGGCAAATTGCGGACCACGCTGTTCATCCCCCACCACGGCGGGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCt                                                       <  1:555864/145‑1 (MQ=255)                                                                                              ttattGTGGCCCCAAACCAGGCCCAAAGGTTGAAATAAGGAAAATGGGGAACCCCGCTTTCCATCCCCCACCACGGCGTTGGAACAAAATAAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTaaa                                                    <  2:144028/148‑1 (MQ=255)                                                                                                      acgcCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGACAGACGGCTAAAAATTTTACAt                                          >  1:223081/2‑150 (MQ=255)                                                                                                           aaaCCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACg                                    >  1:619463/1‑151 (MQ=255)                                                                                                                aTGCCCAAAGGCTTTAATAAGGAAAATTGCGGATCACGCTGTTCATCCCCCCCCCCGGCGGGGGAACAAAATAAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATa                               <  2:734372/151‑1 (MQ=255)                                                                                                                   cgcCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGAc                            >  1:30660/1‑151 (MQ=255)                                                                                                                       aaGGCTGTAAAAAGGAAAATTGCGGATCACGCTGTTCATCCCCCCCCCCGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGAc                            <  2:11278/147‑1 (MQ=255)                                                                                                                         ggCTGTAATAAGGAAAATTGCGGACCACGCTGTTCATCCCCCCCCACGGCGGGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTa                       <  2:89383/150‑1 (MQ=255)                                                                                                                             gaaaaaaGGAAAATGGCGGATCCGGCTGTTCTTCCCCCACCCCGGGGGGGGAACAAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGc                   <  2:364367/145‑1 (MQ=255)                                                                                                                             gTAATAAGGCAAATTGCGGATCACGCTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGc                   >  1:193022/1‑150 (MQ=255)                                                                                                                             gTAATAAGGCAAATTGCGGATCACGCTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGc                   >  2:45170/1‑150 (MQ=255)                                                                                                                                      cAAATTGCGGATCACGCTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATAc         >  1:699343/1‑151 (MQ=255)                                                                                                                                        aaTTGCGGATCACGCTGTTAATCCCCCACCACGGCGGGGAAAAAAAATAAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACca       <  2:175672/151‑1 (MQ=255)                                                                                                                                              ggATCCCGCTGTTCATCCCCCCCCCCGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGc  <  2:131402/150‑1 (MQ=255)                                                                                                                                               gATCCCGCTGTTCATCCCCCACCACGGCGTGGAAACGAAATAAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACaa    <  2:77392/147‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                               AGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGC  >  NC_000913/1174448‑1174738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑