Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,192,714	A→G	C354R (TGC→CGC)	mnmA ←	tRNA(Gln,Lys,Glu) U34 2‑thiouridylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,192,714	0	A	G	100.0%	176.2 / NA	49	C354R (TGC→CGC)	mnmA	tRNA(Gln,Lys,Glu) U34 2‑thiouridylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/15);  total (34/15)

CAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAGGGATATCGGTCTGGCGATAGCGGGTTTTTACCGTGCA  >  NC_000913/1192569‑1192858                                                                                                                                                  |                                                                                                                                                 cAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACtt                                                                                                                                             >  1:736695/1‑151 (MQ=255)   ggCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTc                                                                                                                                            <  2:63906/150‑1 (MQ=255)   ggCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTc                                                                                                                                            >  1:421255/1‑150 (MQ=255)           gACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTtata                                                                                                                                   <  1:645017/151‑1 (MQ=255)                 cAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGAc                                                                                                                             >  2:736123/1‑151 (MQ=255)                 cAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGAc                                                                                                                             >  1:641130/1‑151 (MQ=255)                   aaTACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACgg                                                                                                                           >  2:507644/1‑151 (MQ=255)                      aCCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGc                                                                                                                          >  1:151308/1‑149 (MQ=255)                      aCCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCa                                                                                                                         <  2:742335/150‑1 (MQ=255)                        cGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGAc                                                                                                                      >  2:705552/1‑151 (MQ=255)                         ggCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACt                                                                                                                     >  2:686796/1‑151 (MQ=255)                            cAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGc                                                                                                                  >  1:576360/1‑151 (MQ=255)                             aGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGcc                                                                                                                 <  2:106010/151‑1 (MQ=255)                                     ggTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCa                                                                                                         <  2:56170/151‑1 (MQ=255)                                        gATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCAcg                                                                                                       <  1:307124/150‑1 (MQ=255)                                         aTGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCAcggc                                                                                                     <  2:404578/151‑1 (MQ=255)                                                      tCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTc                                                                                         >  2:427703/1‑150 (MQ=255)                                                      tCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTc                                                                                         <  1:739218/150‑1 (MQ=255)                                                        tttGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATc                                                                                      >  1:747046/1‑151 (MQ=255)                                                         ttGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCa                                                                                        <  2:600307/148‑1 (MQ=255)                                                            ccACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGaa                                                                                   >  1:92702/1‑150 (MQ=255)                                                              aCGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAAt                                                                                >  1:264039/1‑151 (MQ=255)                                                                gTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATc                                                                               >  2:420778/1‑150 (MQ=255)                                                                gTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCa                                                                              <  2:617872/151‑1 (MQ=255)                                                                 ttCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCAc                                                                             >  1:60578/1‑151 (MQ=255)                                                                       gCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCa                                                                         <  2:510593/149‑1 (MQ=255)                                                                       gCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAAt                                                                       >  1:597891/1‑151 (MQ=255)                                                                        cTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCAACCGAGGAGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTAATCGAAAATAACTTCAAt                                                                       >  1:428842/1‑150 (MQ=255)                                                                           ccTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCg                                                                    <  2:73977/150‑1 (MQ=255)                                                                               gTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGatcat                                                               >  1:48075/1‑151 (MQ=255)                                                                               gTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATAACGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGatcat                                                               >  1:521262/1‑151 (MQ=255)                                                                                ttttAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGatcatc                                                              >  1:327365/1‑151 (MQ=255)                                                                                    aaGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTcc                                                          >  1:129381/1‑151 (MQ=255)                                                                                        aaaGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAgcg                                                      >  2:311148/1‑151 (MQ=255)                                                                                        aaaGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAgcg                                                      >  1:19993/1‑151 (MQ=255)                                                                                           gATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCt                                                   >  1:274255/1‑151 (MQ=255)                                                                                            ataataATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCtt                                                  <  2:268755/151‑1 (MQ=255)                                                                                                 aatCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACgg                                             >  2:176091/1‑151 (MQ=255)                                                                                                      gACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCa                                         >  2:773672/1‑150 (MQ=255)                                                                                                             agcggcagACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAGGGatat                                 >  2:482306/1‑151 (MQ=255)                                                                                                                    gACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAGGGATATCGGTCTg                          >  1:9703/1‑151 (MQ=255)                                                                                                                     aCGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAGGGATATCGGTCTgg                         >  2:586430/1‑151 (MQ=255)                                                                                                                         tgctCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGGCCAGCGCCTTGACGGTGCAAGGGATATCGGTCTGTCGAt                     >  1:569078/1‑151 (MQ=255)                                                                                                                               ataataCCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGACGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAGGGATATCGGTCTGGCGATAGCggg               <  2:666269/151‑1 (MQ=255)                                                                                                                                taataCCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAGGGATATCGGTCTGGCGATAGCggg               >  2:146687/1‑150 (MQ=255)                                                                                                                                  ataCCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAGGGATATCGGTCTGGCGATAGCGGGtt             >  1:411876/1‑150 (MQ=255)                                                                                                                                       gCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAGGGATATCGGTCTGGCGATAGCGGGTTTTTAcc       <  2:404722/151‑1 (MQ=255)                                                                                                                                         cACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAGGGATATCGGTCTGGCGATAGCGGGTTTTTACCg      >  1:504953/1‑150 (MQ=255)                                                                                                                                             gAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAGGGATATCGGTCTGGCGATAGCGGGTTTTTACCGTGCa  >  2:381088/1‑150 (MQ=255)                                                                                                                                                  |                                                                                                                                                 CAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAGGGATATCGGTCTGGCGATAGCGGGTTTTTACCGTGCA  >  NC_000913/1192569‑1192858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑