Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	146,238	A→G	K386K (AAA→AAG)	yadE →	putative polysaccharide deacetylase lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	146,238	0	A	G	100.0%	138.4 / NA	39	K386K (AAA→AAG)	yadE	putative polysaccharide deacetylase lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/17);  total (22/17)

ATTTAATCCGCATGTCTGGTATCTTTCGTATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTT  >  NC_000913/146091‑146385                                                                                                                                                    |                                                                                                                                                    aTTTAATCCGCATGTCTGGTATCTTTCGTATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGa                                                                                                                                                  <  1:486587/151‑1 (MQ=255)      aTCCGCATGTCTGGTATCTTTCGTATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTta                                                                                                                                             <  1:303351/151‑1 (MQ=255)      aTCCGCATGTCTGGTATCTTTCGTATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTta                                                                                                                                             <  1:625692/151‑1 (MQ=255)        ccGCATGTCTGGTATCTTTCGTATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTtat                                                                                                                                            <  1:193303/150‑1 (MQ=255)        ccGCATGTCTGGTATCTTTCGTATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTtat                                                                                                                                            <  1:125935/150‑1 (MQ=255)           cATGTCTGGTATCTTTCGTATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCt                                                                                                                                        >  1:412008/1‑151 (MQ=255)                         ttCGTATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGAtt                                                                                                                            <  1:58187/149‑1 (MQ=255)                          tCGTATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTc                                                                                                                           >  1:634261/1‑149 (MQ=255)                                ccGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTgg                                                                                                                      >  1:648867/1‑148 (MQ=255)                                ccGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGgag                                                                                                                    <  1:773975/150‑1 (MQ=255)                                 cGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTGAGAACGGATTCGCTGGAGAc                                                                                                                  >  2:189607/1‑151 (MQ=255)                                       gCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTc                                                                                                            <  2:542767/151‑1 (MQ=255)                                        cGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTcg                                                                                                           <  2:611552/151‑1 (MQ=255)                                             ttAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCt                                                                                                      >  1:414750/1‑151 (MQ=255)                                                aTGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTgg                                                                                                    >  1:309988/1‑150 (MQ=255)                                                 tGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGt                                                                                                   >  1:544533/1‑150 (MQ=255)                                                   aCAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTg                                                                                                  >  2:480614/1‑149 (MQ=255)                                                     aaCGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGCGAGt                                                                                               >  2:741812/1‑150 (MQ=255)                                                       cGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAAc                                                                                            >  2:238613/1‑151 (MQ=255)                                                        gCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAAcc                                                                                           <  1:128984/151‑1 (MQ=255)                                                          cGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAAcc                                                                                           >  1:730107/1‑149 (MQ=255)                                                                                           ggCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCgg                                                        >  1:256713/1‑151 (MQ=255)                                                                                            gCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGa                                                       <  2:684488/151‑1 (MQ=255)                                                                                            gCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGa                                                       <  1:458915/151‑1 (MQ=255)                                                                                             cGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGaa                                                      <  2:479236/151‑1 (MQ=255)                                                                                             cGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGa                                                       >  1:200414/1‑150 (MQ=255)                                                                                              ggTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAAt                                                     >  1:248366/1‑151 (MQ=255)                                                                                                 gACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGc                                                  >  1:712726/1‑151 (MQ=255)                                                                                                    aaCCATGAAAGGCAAAGTAAAACCGGGGGATAATCGGTTGTTGCTAAAGCGCCTTTATAGCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCttt                                               <  1:224077/151‑1 (MQ=255)                                                                                                         tGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCgg                                          >  1:355364/1‑151 (MQ=255)                                                                                                                cAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGttt                                   >  2:570420/1‑151 (MQ=255)                                                                                                                 aaaGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGtttc                                  <  1:570366/151‑1 (MQ=255)                                                                                                                   aGTAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGtttcat                                >  1:274257/1‑151 (MQ=255)                                                                                                                     tAAAACCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGtttcattt                              >  2:739192/1‑151 (MQ=255)                                                                                                                       aaaCCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGtttcatttca                            <  1:759504/151‑1 (MQ=255)                                                                                                                        aaCCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTttcatttcat                           >  1:198898/1‑151 (MQ=255)                                                                                                                         aCCGGGGGATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTtcatttcatt                          >  2:131463/1‑151 (MQ=255)                                                                                                                            gggggATAATCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTc                       <  1:435810/151‑1 (MQ=255)                                                                                                                                   aaTCCGTTGTTACTAAAGCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTc                 >  1:32644/1‑150 (MQ=255)                                                                                                                                                 aaagCGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGtt  <  2:338133/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    ATTTAATCCGCATGTCTGGTATCTTTCGTATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTT  >  NC_000913/146091‑146385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑