Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,265,331	A→G	D107G (GAC→GGC)	prfA →	peptide chain release factor RF‑1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,265,331	0	A	G	100.0%	145.9 / NA	41	D107G (GAC→GGC)	prfA	peptide chain release factor RF‑1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/22);  total (19/22)

CAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTG  >  NC_000913/1265186‑1265474                                                                                                                                                  |                                                                                                                                                cAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAAc                                                                                                                                            >  2:494675/1‑151 (MQ=255)   ggAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACg                                                                                                                                           <  2:37891/150‑1 (MQ=255)    gAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGt                                                                                                                                          >  1:489171/1‑150 (MQ=255)             gAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTcc                                                                                                                                <  2:128723/151‑1 (MQ=255)                      caGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTcc                                                                                                                       <  1:732919/151‑1 (MQ=255)                       aGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCg                                                                                                                      <  1:170532/151‑1 (MQ=255)                        gatgatGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCg                                                                                                                      >  2:391969/1‑150 (MQ=255)                        gatgatGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGa                                                                                                                     <  1:448223/151‑1 (MQ=255)                         atgatgCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAg                                                                                                                    <  1:542900/151‑1 (MQ=255)                               cTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGa                                                                                                               >  1:364656/1‑150 (MQ=255)                                tCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAAc                                                                                                             >  1:598437/1‑151 (MQ=255)                                   atgatCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACcgg                                                                                                          <  1:486741/151‑1 (MQ=255)                                          tGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGcgac                                                                                                   >  1:323193/1‑151 (MQ=255)                                           gAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGcgacg                                                                                                  <  1:42341/151‑1 (MQ=255)                                                   tGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAgcggcg                                                                                          >  1:318976/1‑151 (MQ=255)                                                      gaTGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTg                                                                                       <  1:80057/151‑1 (MQ=255)                                                        tGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGtt                                                                                     >  2:500073/1‑151 (MQ=255)                                                            gcAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTcgcg                                                                                 <  2:410291/151‑1 (MQ=255)                                                             cAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGGAGCGGCGCTGTTCGCgg                                                                                <  1:10374/151‑1 (MQ=255)                                                             cAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCgg                                                                                <  2:269172/151‑1 (MQ=255)                                                               ggATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGc                                                                              <  2:770770/151‑1 (MQ=255)                                                                 aTGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGa                                                                            <  2:660237/151‑1 (MQ=255)                                                                      cTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGt                                                                       >  1:101034/1‑151 (MQ=255)                                                                       tGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGtt                                                                      >  1:409667/1‑151 (MQ=255)                                                                        gcgcGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTc                                                                     >  1:542666/1‑151 (MQ=255)                                                                         cgcgAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTcc                                                                    <  2:412026/151‑1 (MQ=255)                                                                             aaGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTAt                                                                <  2:279558/151‑1 (MQ=255)                                                                              aGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATg                                                               >  2:185564/1‑151 (MQ=255)                                                                                  aaaGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTAc                                                            >  1:621757/1‑150 (MQ=255)                                                                                   aaGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAg                                                          <  2:337226/151‑1 (MQ=255)                                                                                                   aCTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCAGAAGcc                                          <  2:509162/151‑1 (MQ=255)                                                                                                     tGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCccg                                        >  2:562799/1‑151 (MQ=255)                                                                                                       gAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCccgcc                                      <  2:105654/151‑1 (MQ=255)                                                                                                           aGCAATTACAGGTTCTGTTACTGCCACAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTg                                  >  2:543769/1‑151 (MQ=255)                                                                                                                      gTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGGAGaa                        >  2:410870/1‑150 (MQ=255)                                                                                                                              aCTGCCAAAAAATCCTGATGGAGACCTTAACGCCTTCTGCGATTTCCGAGCCGGAACCGGCGGCGACGAGGCGGCGCTGTTCGCGGGAGATCTGTTCTGTATGTACAGCCGTTTTGCCGAAGTCCGCCGCTGGCGGGTAGAAATCATGAg                <  2:526222/150‑1 (MQ=255)                                                                                                                                  ccAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCa           <  2:125559/151‑1 (MQ=255)                                                                                                                                    aaaaGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCCGc         >  1:73770/1‑151 (MQ=255)                                                                                                                                      aaGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGGAGAAATCATGGGCGCCAGc         >  2:2601/1‑149 (MQ=255)                                                                                                                                         aTCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAggg    <  2:316330/151‑1 (MQ=255)                                                                                                                                            cTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGCggggg  >  1:561367/1‑148 (MQ=255)                                                                                                                                                  |                                                                                                                                                CAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTG  >  NC_000913/1265186‑1265474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑