Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,266,451	A→G	T120A (ACC→GCC)	prmC →	RF‑1 and RF‑2 N5‑glutamine methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,266,451	0	A	G	100.0%	118.6 / NA	34	T120A (ACC→GCC)	prmC	RF‑1 and RF‑2 N5‑glutamine methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/17);  total (17/17)

TAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACT  >  NC_000913/1266305‑1266595                                                                                                                                                   |                                                                                                                                                 tAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCgg                                                                                                                                              >  2:1776/1‑151 (MQ=255)           gCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGAttgc                                                                                                                                     <  1:661264/150‑1 (MQ=255)            cGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGAttgcgc                                                                                                                                   >  1:173781/1‑151 (MQ=255)                 aTTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGAttgcgcttgcg                                                                                                                              <  1:201194/151‑1 (MQ=255)                          gTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTagc                                                                                                                     <  1:498423/151‑1 (MQ=255)                             gCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTagcgag                                                                                                                  <  2:125611/151‑1 (MQ=255)                                 ttATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGcc                                                                                                              >  1:670341/1‑151 (MQ=255)                                 ttATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGcc                                                                                                              >  2:295241/1‑151 (MQ=255)                                  tATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGccc                                                                                                             >  2:439861/1‑151 (MQ=255)                                   aTTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCg                                                                                                            >  2:228468/1‑151 (MQ=255)                                   aTTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCAATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCg                                                                                                            >  2:19047/1‑151 (MQ=255)                                     ttGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCgg                                                                                                           >  1:494143/1‑150 (MQ=255)                                            gCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGaa                                                                                                   <  1:155329/151‑1 (MQ=255)                                            gCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGa                                                                                                    >  1:138567/1‑150 (MQ=255)                                                            ccGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATc                                                                                   <  1:161230/151‑1 (MQ=255)                                                             cgcgCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGt                                                                                        >  2:101981/1‑145 (MQ=255)                                                              gcgcCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGt                                                                                 >  1:352762/1‑151 (MQ=255)                                                                gcCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTAt                                                                               <  1:324883/151‑1 (MQ=255)                                                                      aTACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTg                                                                          >  1:686355/1‑150 (MQ=255)                                                                         cGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGc                                                                      >  1:195269/1‑151 (MQ=255)                                                                           gAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTg                                                                    <  1:575727/151‑1 (MQ=255)                                                                                      ggAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGc                                                          <  1:536206/150‑1 (MQ=255)                                                                                       gAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGcac                                                        >  2:363687/1‑151 (MQ=255)                                                                                               aCTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGGGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAAt                                                >  1:355776/1‑151 (MQ=255)                                                                                                  ggCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGc                                              <  2:578247/150‑1 (MQ=255)                                                                                                         ttGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATc                                      >  1:381240/1‑151 (MQ=255)                                                                                                           gCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCt                                     <  1:568195/150‑1 (MQ=255)                                                                                                                   aacCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCaa                            <  1:344502/151‑1 (MQ=255)                                                                                                                            gTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCca                   <  2:172689/151‑1 (MQ=255)                                                                                                                                tctcGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACAt                <  2:329120/150‑1 (MQ=255)                                                                                                                                         cGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGc      <  1:705906/151‑1 (MQ=255)                                                                                                                                         cGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGc      <  2:341539/151‑1 (MQ=255)                                                                                                                                            gACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGAc   <  2:670949/151‑1 (MQ=255)                                                                                                                                              cGGGTGCCGGGGCGCTTCCGCTTGCGCTGGCTAGCTAGCGCCCGGCCTGCGAAATTATCGCTGTAGCTCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATCCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACt  >  1:142134/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                                 TAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACT  >  NC_000913/1266305‑1266595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑