Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,369,520 T→C V43A (GTT→GCT)  pspD → peripheral inner membrane phage‑shock protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,369,5200TC97.6% 130.5 / NA 41V43A (GTT→GCT) pspDperipheral inner membrane phage‑shock protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (11/29);  minor base G (1/0);  total (12/29)
Fisher's exact test for biased strand distribution p-value = 2.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGTTATGAATACTCGCTGGCAACAGGCCGGGCAAAAGGTAAAGCCTGGTTTCAAATTAGCAGGCAAGCTGGTACTTCTTACCGCACTGCGCTATGGCCCGGCGGGTGTGGCGGGCTGGGCGATAAAATCAGTTGCTCGCCGACCGCTGAAAATGTTGCTGGCTGTGGCGCTGGAACCGCTGTTAAGTCGGGCTGCTAATAAACTGGCACAGCGTTATAAAAGGTGAGGGGAGTTTCGCAAAAATTGTTAAATCTCAGGCGTATAATGGATGGCAATT  >  NC_000913/1369388‑1369665
                                                                                                                                     |                                                                                                                                                   
aaGTTATGAATACTCGCTGGCAACAGGCCGGGCAAAAGGTAAAGCCTGGTTTCAAATTAGCAGGCAAGCTGGTACTTCTTACCGCACTGCGCTATGGCCCGGTGGGTGTGGCGGGCTGGGCGATAAAATCAGCTGCTCGCCGACCGCTGaa                                                                                                                                   <  2:79329/151‑1 (MQ=255)
aaGTTATGAATACTCGCTGGCAACAGGCCGGGCAAAAGGTAAAGCCTGGTTTCAAATTAGCAGGCAAGCTGGTACTTCTTACCGCACTGCGCTATGGCCCGGCGGGTGTGGCGGGCTGGGCGATAAAATCAGCTGCTCGCCGACCGCTGaa                                                                                                                                   <  2:659306/151‑1 (MQ=255)
 aGTTATGAATACTCGCTGGCAACAGGCCGGGCAAAAGGTAAAGCCTGGTTTCAAATTAGCAGGCAAGCTGGTACTTCTTACCGCACTGCGCTATGGCCCGGCGGGGGTGGCGGGGGTGGGCGTAAAAACAAGCGCTCCCCGAcccccgg                                                                                                                                    >  1:536368/1‑144 (MQ=255)
    tATGAATACTCGCTGGCAACAGGCCGGGCAAAAGGTAAAGCCTGGTTTCAAATTAGCAGGCAAGCTGGTACTTCTTACCGCACTGCGCTATGGCCCGGCGGGTGTGGCGGGCTGGGCGGTAAAAATAGCTTCTCCCCGACCGCCGgaaa                                                                                                                                 >  2:224553/1‑149 (MQ=255)
               gCTGGCAACAGGCCGGGCAAAAGGTAAAGCCTGGTTTCAAATTAGCAGGCAAGCTGGTACTTCTTACCGCACTGCGCTATGGCCCGGCGGGTGTGGCGGGGGGGGGGGGAAAAACAGCCGCTCGCCCCCCGCCGGAAAAGGTgctgg                                                                                                                        >  1:181168/1‑147 (MQ=255)
                                cAAAAGGTAAAGCCTGGTTTCAAATTAGCAGGCAAGCTGGTACTTCTTACCGCACTGCGCTATGGCCCGGCGGGTGTGGCGGGCTGGGGGGTAAAAATAACTGCTCGCCCCCCCCTGGAAATTTTTCTGGCCGTGGGGCTTGGAcccccgt                                                                                                  >  2:295539/1‑146 (MQ=255)
                                 aaaaGGTAAAGCCTGGTTTCAAATTAGCAGGCAAGCTGGTACTTCTTACCGCACTGCGCTATGGCCCGGCGGGGGTGGGGGGCTGGGGGGTAAAATTACCAGCTCGCCCGCCGCTGGAAAAGTTTGTGGGGTTGGGGCTGGGAGCGCCGtt                                                                                                 >  2:759838/1‑151 (MQ=255)
                                 aaaaGGTAAAGCCTGGTTTCAAATTAGCAGGCAAGATGGTACTTCTTACCGCACTGCGCTATGGCCCGGCGGGTGTGGCGGGCTGGGCGATAAAATCAGCTGCTCGCCGACCGCTGAAAATGTTGCTGGCTGTGGCGCTGGAACCGCTGtt                                                                                                 <  2:184860/151‑1 (MQ=255)
                                               ggTTTCAAATTAGCAGGCAAGCTGGTACTTCTTACCGCACTGCGCTATGGCCCGGCGGGTGTGGCGGGCTGGGCGATAAAATCAGCTGCTCGCCGACCGCTGAAAATGTTGCTGGCTGTGGCGCTGGAACCGCTGTTAAGTCGGgctgct                                                                                    <  2:703665/150‑1 (MQ=255)
                                                   tCAAATTAGCAGGCAAGCTGGTACTTCTTACCGCACTGCGCTATGGCCCGGCGGGTGTGGCGGGCTGGGCGATAAAATCAGCTGCTCGCCGACCGCTGAAAATGTTGCTGGCTGTGGCGCTGGAACCGCTGTTAAGTCGGGCTGCtaata                                                                                <  2:563682/150‑1 (MQ=255)
                                                        ttAGCAGGCAAGCTGGTACTTCTTACCGCACTGCGCTATGGCCCGGCGGGTGTGGCGGGGCGGGGGGTAAAAAAAACCGCTCCCCGCCCGCTGAAAAAGTTGCTGGCTGTGGGGCTGGGACCGCCGTTTAATCGGGGTGGTAATaaaattg                                                                          >  1:88332/1‑147 (MQ=255)
                                                              ggCAAGCTGGTACTTCTTACCGCACTGCGCTATGGCCCGGCGGGTGTGGCGGGCTGGGCGATAAAATCAGCTGCTCGCCGACCGCTGAAAATGTTGCTGGCTGTGGCGCTGGAACCGCTGTTAAGTCGGGCTGCTAATAAACTGGCACAGc                                                                    <  1:352101/151‑1 (MQ=255)
                                                               gCAAGCTGGTACTTCTTACCGCACTGCGCTATGGCCCGGCGGGTGTGGCGGGCTGGGCGATAAAATCAGCTGCTCGCCGACCGCTGAAAATGTTGCTGGCTGTGGCGCTGGAACCGCTGTTAAGTCGGGCTGCTAATAAACTGGCACAGCg                                                                  <  1:594174/151‑1 (MQ=255)
                                                                      ggTACTTCTTACCGCACTGCGCTATGGCCCGGCGGGTGTGGCGGGCTGGGCGATAAAATCAGCTGCTCGCCGACCGCTGAAAATGTTGCTGGCTGTGGCGCTGGAACCGCTGTTAAGTCGGGCTGCTAATAAACTGGCACAGCGTTATaaa                                                           <  1:541468/151‑1 (MQ=255)
                                                                          cttcttACCGCACTGCGCTATGGCCCGGCGGGTGTGGCGGGCTGGGCGATAAAATCAGCTGCTCGCCGACCGCTGAAAATGTTGCTGGCTGTGGCGCTGGAACCGCTGTTAAGTCGGGCTGCTAATAAACTGGCACAGCGTTATAAAAgg                                                        <  1:473404/150‑1 (MQ=255)
                                                                            tcttACCGCACTGCGCTATGGCCCGGCGGGTGTGGCGGGCTGGGCGATAAAATCAGCTGCTCGCCGACCGCTGAAAATGTTGCTGGCTGTGGCGCTGGAACCGCTGTTAAGTCGGGCTGCTAATAAACTGGCACAGCGTTATAAAAGGTg                                                      <  1:350544/150‑1 (MQ=255)
                                                                             cttACCGCACTGCGCTATGGCCCGGCGGGTGTGGCGGGCTGGGGGGTAAAAACAGCTGCTCCCCCACCGCTGGAAAAGGTGCTGGGTGTGGGGCCGGGAACCCCGGTTAATCGGGGTGCTAATAAAATGGGGCAGCGTTATaaaaaggg                                                      >  2:337505/1‑145 (MQ=255)
                                                                             cgtACCGCACTGCGCTATGGGCCGGCGGGTGTGGCGGGCTGGGCGATAAAATCAGCTGCTGGCCGACCGCGGAAAAGGTTGCTGGCGGTGGCGCTGGAACCGCTGTTAAGTCGGGCTGCTAATAAACTGGCACAGCGTTATAAAAGGTGAg                                                    <  1:602024/149‑1 (MQ=255)
                                                                               tACCGCACTGCGCTATGGCCCGGCGGGTGTGGCGGGCTGGGCGATAAAATCAGCTGCTCGCCGACCGCTGAAAATGTTGCTGGCTGTGGCGCTGGAACCGCTGTTAAGTCGGGCTGCTAATAAACTGGCACAGCGTTATAAAAGGTGa                                                     <  2:400910/148‑1 (MQ=255)
                                                                               tACCGCACTGCGCTATGGCCCGGCGGGTGTGGCGGGCTGGGCGATAAAATCAGCTGCTCGCCGACCGCTGAAAATGTTGCTGGCTGTGGCGCTGGAACCGCTGTTAAGTCGGGCTGCTAATAAACTGGCACAGCGTTATAAAAGGTGAgg                                                   <  1:653033/150‑1 (MQ=255)
                                                                               tACCGCACTGCGCTATGGCCCGGCGGGTGTGGCGGGCTGGGCGATAAAATCAGCTGCTCGCCGACCGCTGAAAATGTTGCTGGCTGTGGCGCTGGAACCGCTGTTAAGTCGGGCTGCTAATAAACTGGCACAGCGTTATAAAAGGTGAg                                                    <  2:101842/149‑1 (MQ=255)
                                                                                 ccGCACTGCGCTATGGCCCGGCGGGTGTGGCGGGCTGGGGGGTAAAAAAAGCCGCTCGCCCCCCGCCGGAAAAGTTTTTGGGTGGGGGGCCGGGACCGCTGTTTAGTCGGGGTGCTAATAAAATGGGACAGCGGTTTTAAAAGgggggggg                                                 >  1:650506/1‑151 (MQ=255)
                                                                                    cACTGCGCTATGGCCCGGCGGGTGTGGCGGGCTGGGCGATAAAATCAGCTGCTCGCCGACCGCTGAAAATGTTGCTGGCTGTGGCGCTGGAACCGCTGTTAAGTCGGGCTGCTAATAAACTGGCACAGCGTTATAAAAGGTGAGGGGAGtt                                             <  2:757361/151‑1 (MQ=255)
                                                                                        gcgcTATGGCCCGGCGGGTGTGGCGGGGCGGGCGATAAAAACAGCCTCTCGCCCACCCCCGGAAAAGTTGCTGGGGGGGGGGCCGGGAACGCTGGTTAGTCGGGCCGCTAATAAAATTGCACAGCCTTTTTAAAAGGGAgggggggtt                                            >  1:367650/1‑143 (MQ=255)
                                                                                        gcgcTATGGCCCGGCGGGTGTGGCGGGCTGGGCGATAAAATCAGCTGCTCGCCGACCGCTGAAAATGTTGCTGGCTGTGGCGCTGGAACCGCTGTTAAGTCGGTCTGCTAATAAACTGGCACAGCGTTATAAAAGGTGAGGGGAGTTTCg                                          <  1:104145/150‑1 (MQ=255)
                                                                                        gcgcTATGGCCCGGCGGGTGTGGCGGGCTGGGCGATAAAATCAGCTGCTCGCCGACCGCTGAAAATGTTGCTGGCTGTGGCGCTGGAACCGCTGTTAAGTCGGGCTGCTAATAAACTGGCACAGCGTTATAAAAGGTGAGGGGAGTTTCg                                          <  1:56568/150‑1 (MQ=255)
                                                                                               ggCCCGGCGGGTGTGGCGGGCTGGGCGATAAAATCAGCTGCTCGCCGACCGCTGAAAATGTTGCTGGCTGTGGCGCTGGAACCGCTGTTAAGTCGGGCTGCTAATAAACTGGCACAGCGTTATAAAAGGTGAGGGGAGTTTCGCAAAAAtt                                  <  2:38907/151‑1 (MQ=255)
                                                                                                 cccGGCGGGTGTGGCGGGCTGGGCGATAAAATCAGCTGCTCGCCGACCGCTGAAAATGTTGCTGGCTGTGGCGCTGGAACCGCTGTTAAGTCGGGCTGCTAATAAACTGGCACAGCGTTATAAAAGGTGAGGGGAGTTTCGCAAAAATTGt                                <  2:293914/151‑1 (MQ=255)
                                                                                                  ccGGCGGGTGTGGCGGGCTGGGCGATAAAATCAGCTGCTCGCCGACCGCTGAAAATGTTGCTGGCTGTGGCGCTGGAACCGCTGTTAAGTCAGGCTGCTAATAAACTGGCACAGCGTTATAAAAGGTGAGGGGAGTTTCGCAAAAATTGtt                               <  1:31765/151‑1 (MQ=255)
                                                                                                   cggcggGTGTGGCGGGCTGGGCGATAAAATCAGCTGCTCGCCGACCGCTGAAAATGTTGCTGGCTGTGGCGCTGGAACCGCTGTTAAGTCGGGCTGCTAATAAACTGGCACAGCGTTATAAAAGGTGAGGGGAGTTTCGCAAAAATTGTTa                              <  1:535288/151‑1 (MQ=255)
                                                                                                      cggGTGTGGCGGGCTGGGCGATAAAATCAGCTGCTCGCCGACCGCTGAAAATGTTGCTGGCTGTGGCGCTGGAACCGCTGTTAAGTCGGGCTGCTAATAAACTGGCACAGCGTTATAAAAGGTGAGGGGAGTTTCGCAAAAATTGTTAAAt                           <  2:650564/151‑1 (MQ=255)
                                                                                                       gggTGTGGCGGGCTGGGCGATAAAATCAGCTGCTCGCCCACCGCTGGAAATGTTGCTGGCTGTGGCGCTGGAACCGCTGTTTAATCGGGCTGCTAATAAAATGGCACAGCGTTATAAAAGGGGAGGGGAGGTTCGCAAAAAATGTTAAAt                           >  1:288674/1‑150 (MQ=255)
                                                                                                           gtgGCGGGCTGGGCGATAAAATCAGCTGCTCGCCGACCGCTGAAAATGTTGCTGGCTGTGGCGCTGGAACCGCTGTTAAGTCGGGCTGCTAATAAACTGGCACAGCGTTATAAAAGGTGAGGGGAGTTTCGCAAAAATTGTTAAATCTCAg                      <  1:107336/151‑1 (MQ=255)
                                                                                                            tgGCGGGCTGGGCGATAAAATCAGCTGCTCGCCGACCGCTGAAAATGTTGCTGGCTGTGGCGCTGGAACCGCTGTTAAGTCGGGCTGCTAATAAACTGGCACAGCGTTATAAAAGGTGAGGGGAGTTTCGCAAAAATTGTTAAATCTCAgg                     >  1:360575/1‑151 (MQ=255)
                                                                                                                 gggCTGGGCGATAAAATCAGCTGCTCGCCGACCGCTGAAAATGTTGCTGGCTGTGGCGCTGGAACCGCTGTTAAGTCGGGCTGCTAATAAACTGGCACAGCGTTATAAAAGGTGAGGGGAGTTTCGCAAAAATTGTTAAATCTCAGGCGta                 <  2:146623/151‑1 (MQ=255)
                                                                                                                  ggCTGGGCGATAAAATCAGCTGCTCGCCGACCGCTGAAAATGTTGCTGGCTGTGGCGCTGGAACCGCTGTTAAGTCGGGCTGCTAATAAACTGGCACAGCGTTATAAAAGGTGAGGGGAGTTTCGCAAAAATTGTTAAATCTCAGGCGtat                <  2:722106/151‑1 (MQ=255)
                                                                                                                         cGATAAAATCAGCTGCTCGCCGACCGCTGAAAATGTTGCTGGCTGTGGCGCTGGAACCGCTGTTAAGTCGGGCTGCTAATAAACTGGCACAGCGTTATAAAAGGTGAGGGGAGTTTCGCAAAAATTGTTAAATCTCAGGCGTATAatggat         >  1:85221/1‑151 (MQ=255)
                                                                                                                         cGATAAAATCAGCTGCTCGCCGACCGCTGAAAATGTTGCTGGCTGTGGCGCTGGAACCGCTGTTAAGTCGGGCTGCTAATAAACTGGCACAGCGTTATAAAAGGTGAGGGGAGTTTCGCAAAAATTGTTAAATCTCAGGCGTATAatggat         <  2:73071/151‑1 (MQ=255)
                                                                                                                             aaaaTCAGCTGCTCGCCGACCGCTGAAAATGTTGCTGGCTGTGGCGCTGGAACCGCTGTTAAGTCGGGCTGCTAATAAACTGGCACAGCGTTATAAAAGGTGAGGGGAGTTTCGCAAAAATTGTTAAATCTCAGGCGTATAATGGATGGc      <  2:577024/150‑1 (MQ=255)
                                                                                                                               aaTCAGCTGCTCGCCGACCGCTGAAAATGTTGCTGGCTGTGGCGCTGGAACCGCTGTTAAGTCGGGCTGCTAATAAACTGGCACAGCGTTATAAAAGGTGAGGGGAGTTTCGCAAAAATTGTTAAATCTCAGGCGTATAATGGATGGCAAt   <  1:530443/151‑1 (MQ=255)
                                                                                                                                aTCAGCTGCGCGCCGACCGCTGAAAATGATGCTGGCTGTGGCGCTGGAACCGCTGTTAAGTCGGGCTGCTAATAAACTGGCACAGCTATATAAAAGGTGAGGGGAGTTTCGCAAAAATTGTTAAATCTCAGGCGTATCATGGATGGCCAtt  <  1:416698/151‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                                   
AAGTTATGAATACTCGCTGGCAACAGGCCGGGCAAAAGGTAAAGCCTGGTTTCAAATTAGCAGGCAAGCTGGTACTTCTTACCGCACTGCGCTATGGCCCGGCGGGTGTGGCGGGCTGGGCGATAAAATCAGTTGCTCGCCGACCGCTGAAAATGTTGCTGGCTGTGGCGCTGGAACCGCTGTTAAGTCGGGCTGCTAATAAACTGGCACAGCGTTATAAAAGGTGAGGGGAGTTTCGCAAAAATTGTTAAATCTCAGGCGTATAATGGATGGCAATT  >  NC_000913/1369388‑1369665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: