| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,435,623 | A→G | intergenic (‑4/+137) | uspF ← / ← ompN | stress‑induced protein, ATP‑binding protein/outer membrane pore protein N, non‑specific |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,435,623 | 0 | A | G | 94.1% | 177.5 / ‑6.1 | 51 | intergenic (‑4/+137) | uspF/ompN | stress‑induced protein, ATP‑binding protein/outer membrane pore protein N, non‑specific |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (25/23); minor base C (0/3); total (25/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAGTAGGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTG > NC_000913/1435480‑1435768 | tagtagGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGACAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTg > 2:149103/1‑151 (MQ=255) agGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGttt < 2:536386/150‑1 (MQ=255) gggCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTa < 2:600690/151‑1 (MQ=255) gCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGt < 1:723255/151‑1 (MQ=255) gTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATc < 2:90977/151‑1 (MQ=255) aaGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGc > 1:318159/1‑151 (MQ=255) aaGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGc > 1:382542/1‑151 (MQ=255) ggTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCAt < 1:519542/151‑1 (MQ=255) aTTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATaaa < 1:674251/151‑1 (MQ=255) tACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAg < 2:2485/150‑1 (MQ=255) aGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGc < 1:138573/151‑1 (MQ=255) aaaTGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGcc > 2:450066/1‑149 (MQ=255) aaTGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGcc > 1:696019/1‑148 (MQ=255) gAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATg < 2:318018/150‑1 (MQ=255) aaCCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGac < 2:56871/151‑1 (MQ=255) gCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACttttt < 2:169529/151‑1 (MQ=255) gCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACttt < 2:211332/149‑1 (MQ=255) atcatcAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTtgt < 2:247604/151‑1 (MQ=255) tcatcaATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTtgtg > 1:246360/1‑151 (MQ=255) tcaATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTa > 2:453321/1‑150 (MQ=255) aaTCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATg < 2:674799/151‑1 (MQ=255) ttGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACgg > 2:98919/1‑150 (MQ=255) tGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACgg > 1:313135/1‑149 (MQ=255) ccTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGtt > 2:537460/1‑151 (MQ=255) ctTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTcac > 1:520272/1‑151 (MQ=255) tCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGTAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACTCCAGATGACACTTTTTGTGTAATGACGGAGTTca < 2:78867/148‑1 (MQ=255) tCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACAt > 1:34309/1‑151 (MQ=255) cAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACAttttt > 2:371257/1‑151 (MQ=255) tGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAAttt > 2:208291/1‑151 (MQ=255) ggCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTa < 2:16216/151‑1 (MQ=255) gCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAg < 2:313168/151‑1 (MQ=255) aaTCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGa < 1:721596/149‑1 (MQ=255) aCGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAag < 2:182169/151‑1 (MQ=255) aCGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAag < 2:117630/151‑1 (MQ=255) gTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAaggagg > 2:508079/1‑151 (MQ=255) gAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAggagga > 2:397646/1‑149 (MQ=255) aGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGgaaga < 2:728446/151‑1 (MQ=255) aaTCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGaa > 2:580704/1‑150 (MQ=255) cGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGaaaa > 2:348869/1‑149 (MQ=255) aaTATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCg < 2:246393/151‑1 (MQ=255) atatCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGc > 1:480634/1‑151 (MQ=255) cGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCAt > 1:634443/1‑150 (MQ=255) gATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATa > 2:95035/1‑150 (MQ=255) aTAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAAc > 2:411455/1‑151 (MQ=255) tAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAAc > 2:82755/1‑150 (MQ=255) attctttttGTTAAAAAACCCTCTTTTTTTAGTATCCAAATAAAGTTTAACGCCAAATGACATTTTTTGTGTAAGAACGGATTCCACTTTTTTAATTAAGAACAAAGGGGAAAAAATAAGAAAAAAAAGCCCGCCATAACAGCGGGCag < 1:533491/141‑1 (MQ=255) aTCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCagg > 1:6654/1‑149 (MQ=255) tctttCTTTTAAAAAACCCTCTTTTTTTAGTACCCAAAAAAAGTGTAACCCAAGATGACTTTTTTTGTGAAAGGACGGATTTCACTTTTTTAATTAAGAAAAAGGGAGAAAAAAAAAGAAAAAAAAGCCCGCCATAACAGCGGGCaggag < 1:397597/147‑1 (MQ=255) ttttttaaaaaaagCCTCTTTTTTTATTATCCAAAAAAGTTGTAACGCAAGTTGATTGTTTTTGAGAAATGAGGGATTTCAATTTTTTAATTTAGAGAAAGGGAGAAAAAAAAAGAAAAAAAAGCCCGCCATAACAGCGGGCaggagg < 1:249453/138‑1 (MQ=255) ttttGTTAAAAAACCCTCTTTTTTTAGTACCCGAAAAAAGTGAAACGCAAGAGGACATTTTTTGTGTAATGGCGGAGTCCACATTTTTAATTTAGATCAAGGGAGGAAGAATAAGAAGAAAAAGCCCGCCATAACAGCGGGCaggag < 2:318192/144‑1 (MQ=255) ttCTGTTAAAAAAGCCTCTTTTTTTAGTACCCGCATAAAGTGAACCGCCAGATGACATTTTTTGTGTAATGGCGAATTCCACTTTTTAAATTTAGAAAAAAGGAGAAAAAATAAGCAAAAAAAGCCCGCCATAACAGCGGGCaggagg < 1:182821/148‑1 (MQ=255) aTAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTg > 1:100456/1‑151 (MQ=255) | TAGTAGGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTG > NC_000913/1435480‑1435768 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |