Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	150,128	A→G	S276P (TCT→CCT)	yadC ←	putative fimbrial‑like adhesin protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	150,128	0	A	G	100.0%	182.2 / NA	52	S276P (TCT→CCT)	yadC	putative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/23);  total (29/23)

TTCCAGTAAGCGTATTACCAAGCAGTTGTGTGTTTTGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGAGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACATTATTCAGTGTGAAATTGACATAAATGTGGTTGCTGTGATCTACAACTTTTGTA  >  NC_000913/149981‑150270                                                                                                                                                    |                                                                                                                                               ttCCAGTAAGCGTATTACCAAGCAGTTGTGTGTTTTGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCt                                                                                                                                             >  2:141645/1‑151 (MQ=255)   ccAGTAAGCGTATTACCAAGCAGTTGTGTGTTTTGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCta                                                                                                                                            <  1:722335/150‑1 (MQ=255)      gTAAGCGTATTACCAAGCAGTTGTGTGTTTTGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATAtt                                                                                                                                        <  2:315228/151‑1 (MQ=255)       tAAGCGTATTACCAAGCAGTTGTGTGTTTTGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATAtt                                                                                                                                        >  2:437455/1‑150 (MQ=255)       tAAGCGTATTACCAAGCAGTTGTGTGTTTTGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATtc                                                                                                                                       >  1:167584/1‑151 (MQ=255)           cGTATTACCAAGCAGTTGTGTGTTTTGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTccc                                                                                                                                   >  1:339394/1‑151 (MQ=255)               ttACCAAGCAGTTGTGTGTTTTGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCAtt                                                                                                                                >  1:149089/1‑150 (MQ=255)               ttACCAAGCAGTTGTGTGTTTTGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTc                                                                                                                               >  2:714803/1‑151 (MQ=255)                 aCCAAGCAGTTGTGTGTTTTGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCt                                                                                                                              <  1:148262/150‑1 (MQ=255)                 aCCAAGCAGTTGTGTGTTTTGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCt                                                                                                                              <  1:570062/150‑1 (MQ=255)                    aaGCAGTTGTGTGTTTTGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAAcc                                                                                                                          >  1:761087/1‑151 (MQ=255)                     aGCAGTTGTGTGTTTTGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCg                                                                                                                         <  2:523195/151‑1 (MQ=255)                      gCAGTTGTGTGTTTTGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGt                                                                                                                        >  1:337638/1‑151 (MQ=255)                       cAGTTGTGTGTTTTGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGtt                                                                                                                       <  2:610104/151‑1 (MQ=255)                            gtgtgtTTTGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAAc                                                                                                                   <  2:475585/150‑1 (MQ=255)                             tgtgtTTTGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAAcc                                                                                                                  >  1:542649/1‑150 (MQ=255)                             tgtgtTTTGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAAcc                                                                                                                  >  1:645550/1‑150 (MQ=255)                                gtTTTGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTg                                                                                                              >  2:50064/1‑151 (MQ=255)                                      aGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGAt                                                                                                        >  1:716743/1‑151 (MQ=255)                                         aCCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGc                                                                                                     >  1:436595/1‑151 (MQ=255)                                               ttttCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGt                                                                                                >  2:108073/1‑150 (MQ=255)                                                  tCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGt                                                                                            >  2:691965/1‑151 (MQ=255)                                                        gACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAg                                                                                      <  1:130200/151‑1 (MQ=255)                                                                    ttCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGtt                                                                          >  1:666144/1‑151 (MQ=255)                                                                    ttCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGt                                                                           <  2:303593/150‑1 (MQ=255)                                                                      cAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTg                                                                         <  2:617861/150‑1 (MQ=255)                                                                              cGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTa                                                                <  2:189472/151‑1 (MQ=255)                                                                                 acacGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAAtt                                                             <  2:691540/151‑1 (MQ=255)                                                                                   acGAATCCAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAAttt                                                            >  1:339857/1‑150 (MQ=255)                                                                                     gAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGAc                                                         <  2:155016/151‑1 (MQ=255)                                                                                      aaTACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGAc                                                         >  2:525132/1‑150 (MQ=255)                                                                                        tACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACatt                                                      >  1:389215/1‑151 (MQ=255)                                                                                        tACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACatt                                                      >  1:49153/1‑151 (MQ=255)                                                                                            aTTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGGAAGGTGAGTTTGACATTATTc                                                  <  1:117720/151‑1 (MQ=255)                                                                                                tGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACATTATTCAgtg                                              >  1:189977/1‑151 (MQ=255)                                                                                                      gagaTATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACATTATTCAGTGTGAAAt                                        >  1:115973/1‑151 (MQ=255)                                                                                                         atatCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGTCTGATGGCCCGGTAAGTATGGAAGTAAAACATGTTGGTAAGGTTAATTTGACATTATTCAGTGTGAAATTGa                                     <  1:628604/151‑1 (MQ=255)                                                                                                              aaaGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACATTATTCAGTGTGAAATTGACATa                                 >  2:143195/1‑150 (MQ=255)                                                                                                              aaaGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACATTATTCAGTGTGAAATTGACATa                                 >  1:25274/1‑150 (MQ=255)                                                                                                                    aaCAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACATTATTCAGTGTGAAATTGACATAAATGTgg                          <  2:85416/151‑1 (MQ=255)                                                                                                                     aCAGGTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACATTATTCAGTGTGAAATTGACATAAATGTGGt                         <  2:202544/151‑1 (MQ=255)                                                                                                                        ggTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACATTATTCAGTGTGAAATTGACATAAATGTGGTTGc                      <  1:434593/151‑1 (MQ=255)                                                                                                                         gTGAAGCGCCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACATTATTCAGTGTGAAATTGACATAAATGTGGTTGCt                     >  2:167283/1‑151 (MQ=255)                                                                                                                              gcgcCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACATTATTCAGTGTGAAATTGACATAAATGTGGTTGCTGTGAt                <  2:644913/151‑1 (MQ=255)                                                                                                                              gcgcCATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACATTATTCAGTGTGAAATTGACATAAATGTGGTTGCTGTGAt                >  1:719780/1‑151 (MQ=255)                                                                                                                                 ccATTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACATTATTCAGTGTGAAATTGACATAAATGTGGTTGCTGTGATCTa             <  1:717832/151‑1 (MQ=255)                                                                                                                                   aTTTTTAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACATTATTCAGTGTGAAATTGACATAAATGTGGTTGCTGTGATCTAc            >  2:6324/1‑150 (MQ=255)                                                                                                                                    tttttAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACATTATTCAGTGTGAAATTGACATAAATGTGGTTGCTGTGATCTACa           >  2:85384/1‑150 (MQ=255)                                                                                                                                      tttAATTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACATTATTCAGTGTGAAATTGACATAAATGTGGTTGCTGTGATCTACAAc         <  2:414590/150‑1 (MQ=255)                                                                                                                                          aTTGTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACATTATTCAGTGTGAAATTGACATAAATGTGGTTGCTGTGATCTACAACTTTTg    >  1:662219/1‑151 (MQ=255)                                                                                                                                             gTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACATTATTCAGTGTGAAATTGACATAAATGTGGTTGCTGTGATCTACAACTTTTGTa  <  2:531587/150‑1 (MQ=255)                                                                                                                                             gTCCCAGGGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACATTATTCAGTGTGAAATTGACATAAATGTGGTTGCTGTGATCTACAACTTTTGTa  <  2:69925/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                               TTCCAGTAAGCGTATTACCAAGCAGTTGTGTGTTTTGAGTACCTACTTTTCCAGTGACGAGTTTTGTTTCAATATTACGCACACGAATACAATTCTGAAGCGAGATATCAAAGGGAACAGGTGAAGCGCCATTTTTAATTGTCCCAGAGCTATATTCTCCCATTCTAACCGTTGAACCATTGACTGATGGCCCGGTAAGTATGGAGGTAAAACATGTTGGTAAGGTTAATTTGACATTATTCAGTGTGAAATTGACATAAATGTGGTTGCTGTGATCTACAACTTTTGTA  >  NC_000913/149981‑150270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑