Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,474,495	A→G	D92G (GAC→GGC)	ydbC →	putative NAD(P)‑binding oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,474,495	0	A	G	100.0%	187.7 / NA	56	D92G (GAC→GGC)	ydbC	putative NAD(P)‑binding oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/21);  total (35/21)

GCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGACGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTGCGCGTTATGATGGGGGATGGTCATGGCCCAGCGGAAGGATCGAT  >  NC_000913/1474353‑1474639                                                                                                                                               |                                                                                                                                                 gcgcTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCt                                                                                                                                          >  2:117934/1‑151 (MQ=255)    cTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTgg                                                                                                                                        >  2:183258/1‑150 (MQ=255)    cTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTACGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGt                                                                                                                                       >  2:699115/1‑151 (MQ=255)     tggcgcggggCGGCAATCATATTGATACCAGCGACTTTTGTTGTCCGCACGTCACCAACCAGATTATCAGCGAAGCGCTTTATCTTTACTCTGACGCCCTGACAATTGTCACTAAAATTGGCGCGCGGCGTGGTGAGGGCGCATCCTGGtt                                                                                                                                      >  1:558476/8‑151 (MQ=255)       gCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGc                                                                                                                                    >  1:728486/1‑151 (MQ=255)           tGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGCGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGc                                                                                                                                >  1:507735/1‑151 (MQ=255)               cGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCAttt                                                                                                                            >  2:417072/1‑151 (MQ=255)                gTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCAtttt                                                                                                                           <  1:149138/151‑1 (MQ=255)                  cAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTtct                                                                                                                         >  2:645472/1‑151 (MQ=255)                  cAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTtc                                                                                                                          <  1:556091/150‑1 (MQ=255)                   aaTCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTtct                                                                                                                         >  2:2639/1‑150 (MQ=255)                         atTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCa                                                                                                                   <  1:594084/150‑1 (MQ=255)                          ttGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGc                                                                                                                    >  2:111820/1‑148 (MQ=255)                            gATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGc                                                                                                               >  2:603435/1‑151 (MQ=255)                             atgccAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCt                                                                                                              <  1:682633/148‑1 (MQ=255)                               aCCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTg                                                                                                             <  1:397883/150‑1 (MQ=255)                               aCCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGc                                                                                                            >  2:300425/1‑151 (MQ=255)                                ccAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCa                                                                                                           <  2:520939/151‑1 (MQ=255)                                ccAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCa                                                                                                           <  1:63912/151‑1 (MQ=255)                                 cAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCaa                                                                                                          <  1:189573/151‑1 (MQ=255)                                   gCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCaaaa                                                                                                        <  1:721745/151‑1 (MQ=255)                                     gACTTTTATGGTCCGCACGTCACCAATCAGATTATCCCCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCaaaaa                                                                                                       <  1:208062/150‑1 (MQ=255)                                      aCTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGc                                                                                                     >  1:502526/1‑151 (MQ=255)                                      aCTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGc                                                                                                     <  1:179068/151‑1 (MQ=255)                                       cTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCg                                                                                                    >  1:488860/1‑151 (MQ=255)                                                 ccGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATa                                                                                          <  1:168373/151‑1 (MQ=255)                                                     aCGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCt                                                                                      >  1:688716/1‑151 (MQ=255)                                                       gTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTAc                                                                                    <  1:612828/151‑1 (MQ=255)                                                         cACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACg                                                                                   <  1:102583/150‑1 (MQ=255)                                                         cACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGt                                                                                  <  2:38576/151‑1 (MQ=255)                                                                cAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCg                                                                           >  2:488779/1‑151 (MQ=255)                                                                cAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCg                                                                           <  2:598489/151‑1 (MQ=255)                                                                 aGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCgg                                                                          >  1:222028/1‑151 (MQ=255)                                                                  gATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCggg                                                                         >  1:449107/1‑151 (MQ=255)                                                                    ttATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCt                                                                       >  1:44479/1‑151 (MQ=255)                                                                         cgcgAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACg                                                                  <  1:699987/151‑1 (MQ=255)                                                                          gcgAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGt                                                                 >  1:286684/1‑151 (MQ=255)                                                                           cgAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTg                                                                >  1:335309/1‑151 (MQ=255)                                                                             aaGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCt                                                              >  2:557118/1‑151 (MQ=255)                                                                              aGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTg                                                             <  2:699164/151‑1 (MQ=255)                                                                                 gcTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGAt                                                          >  1:531978/1‑151 (MQ=255)                                                                                   tttATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGAtgt                                                        >  1:307416/1‑151 (MQ=255)                                                                                   tttATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGAtg                                                         >  1:39988/1‑150 (MQ=255)                                                                                    ttATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGAtgtg                                                       >  2:189899/1‑151 (MQ=255)                                                                                      aTCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGt                                                     >  2:490189/1‑151 (MQ=255)                                                                                        ccTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGtt                                                    >  2:20342/1‑150 (MQ=255)                                                                                         cTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTa                                                   >  1:336058/1‑150 (MQ=255)                                                                                            aCTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAAcc                                                <  2:631319/150‑1 (MQ=255)                                                                                                    gacCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTGCGCGTTa                                       <  1:708445/151‑1 (MQ=255)                                                                                                      ccTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTGCGCGTTatg                                     >  2:382251/1‑151 (MQ=255)                                                                                                      acTGACAATTGATACTAAGATTGGTGCGCGGCGTGGAGAGTGCGCATCCTGAGTGCCCGCATTTTATCCGGCAGAGCTGCAAAGAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGCTAACCTGCAGGTTat                                      >  1:223523/2‑150 (MQ=255)                                                                                                        tGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTGCGCGTTatgat                                   >  1:752361/1‑151 (MQ=255)                                                                                                                      aaaaTTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTGCGCGTTATGATGGGGGATGGTCa                       <  2:96761/149‑1 (MQ=255)                                                                                                                        aaTTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTGCGCGTTATGATGGGGGATGGTCATGGc                   >  1:616855/1‑151 (MQ=255)                                                                                                                                     cgTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTGCGCGTTATGATGGGGGATGGTCATGGCCCAGCGGAAGGAt      >  1:751635/1‑151 (MQ=255)                                                                                                                                          agagGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTGCGCGTTATGATGGGGGATGGTCATGGCCCAGCGGAAGGATCGAt  >  2:352507/1‑150 (MQ=255)                                                                                                                                               |                                                                                                                                                 GCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGACGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTGCGCGTTATGATGGGGGATGGTCATGGCCCAGCGGAAGGATCGAT  >  NC_000913/1474353‑1474639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑