Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,526,410 C→T T55I (ACA→ATA)  yncG → glutathione S‑transferase homolog

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,526,4100CT97.9% 175.8 / ‑4.2 48T55I (ACA→ATA) yncGglutathione S‑transferase homolog
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (28/19);  total (28/20)
Fisher's exact test for biased strand distribution p-value = 4.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

CGTACCCGGATGGGGCTCTACAATCAGTGAGCTGATGCTAACACTGGCTGATATTCCTTATCAGTTCGTTGATGTCAGTGGTTTTGACCACGAGGGAGCTTCACGCGAGTTATTGAAAACCCTGAACCCGTTGTGTCAGGTTCCGACACTGGCATTGGAAAATGACGAGATTATGACGGAGACAGCGGCGATTGCATTGATGGTTCTCGATCGTCGCCCGGACCTTGCGCCACCGGTTGGGCGCGCCGAACGTCAGCTGTTTCAACGGCTATTGGTCTGGCTGGTTGCTAATGT  >  NC_000913/1526264‑1526557
                                                                                                                                                  |                                                                                                                                                   
cGTACCCGGATGGGGCTCTACAATCAGTGAGCTGATGCTAACACTGGCTGATATTCCTTATCAGTTCGTTGATGTCAGTGGTTTTGACCACGAGGGAGCTTCACGCGAGTTATTGAAAACCCTGAACCCGTTGTGTCAGGTTCCGACACTg                                                                                                                                                 <  2:157608/151‑1 (MQ=255)
 gTACCCGGATGGGGCTCTACAATCAGTGAGCTGATGCTAACACTGGCTGATATTCCTTATCAGTTCGTTGATGTCAGTGGTTTTGACCACGAGGGAGCTTCACGCGAGTTATTGAAAACCCTGAACCCGTTGTGTCAGGTTCCGATACTgg                                                                                                                                                <  2:319248/151‑1 (MQ=255)
       ggATGGGGCTCTACAATCAGTGAGCTGATGCTAACACTGGCTGATATTCCTTATCAGTTCGTTGATGTCAGTGGTTTTGACCACGAGGGAGCTTCACGCGAGTTATTGAAAACCCTGAACCCGTTGTGTCAGGTTCCGATACTGGCATTgg                                                                                                                                          <  2:83705/151‑1 (MQ=255)
         aTGGGGCTCTACAATCAGTGAGCTGATGCTAACACTGGCTGATATTCCTTATCAGTTCGTTGATGTCAGTGGTTTTGACCACGAGGGAGCTTCACGCGAGTTATTGAAAACCCTGAACCCGTTGTGTCAGGTTCCGATACTGGCATTGGa                                                                                                                                         >  2:420337/1‑150 (MQ=255)
             ggCTCTACAATCAGTGAGCTGATGCTAACACTGGCTGATATTCCTTATCAGTTCGTTGATGTCAGTGGTTTTGACCACGAGGGAGCTTCACGCGAGTTATTGAAAACCCTGAACCCGTTGTGTCAGGTTCCGATACTGGCATTGGAAAATg                                                                                                                                    <  2:64059/151‑1 (MQ=255)
              gCTCTACAATCAGTGAGCTGATGCTAACACTGGCTGATATTCCTTATCAGTTCGTTGATGTCAGTGGTTTTGACCACGAGGGAGCTTCACGCGAGTTATTGAAAACCCTGAACCCGTTGTGTCAGGTTCCGATACTGGCATTGGAAAATg                                                                                                                                    >  1:149713/1‑150 (MQ=255)
                tctACAATCAGTGAGCTGATGCTAACACTGGCTGATATTCCTTATCAGTTCGTTGATGTCAGTGGTTTTGACCACGAGGGAGCTTCACGCGAGTTATTGAAAACCCTGAACCCGTTGTGTCAGGTTCCGATACTGGCATTGGAAAATGACg                                                                                                                                 >  1:314715/1‑151 (MQ=255)
                 ctACAATCAGTGAGCTGATGCTAACACTGGCTGATATTCCTTATCAGTTCGTTGATGTCAGTGGTTTTGACCACGAGGGAGCTTCACGCGAGTTATTGAAAACCCTGAACCCGTTGTGTCAGGTTCCGATACTGGCATTGGAAAATGAc                                                                                                                                  <  1:611064/149‑1 (MQ=255)
                   aCAATCAGTGAGCTGATGCTAACACTGGCTGATATTCCTTATCAGTTCGTTGATGTCAGTGGTTTTGACCACGAGGGAGCTTCACGCGAGTTATTGAAAACCCTGAACCCGTTGTGTCAGGTTCCGATACTGGCATTGGAAAATGACg                                                                                                                                 >  2:246904/1‑148 (MQ=255)
                     aaTCAGTGAGCTGATGCTAACACTGGCTGATATTCCTTATCAGTTCGTTGATGTCAGTGGTTTTGACCACGAGTGAGCTTCACGCGAGTTATTGAAAACCCTGAACCCGTTGTGTCAGGTTCCGATACTGGCATTGGAAAATGACGAGAtt                                                                                                                            >  2:667733/1‑151 (MQ=255)
                        cAGTGAGCTGATGCTAACACTGGCTGATATTCCTTATCAGTTCGTTGATGTCAGTGGTTTTGACCACGAGGGAGCTTCACGCGAGTTATTGAAAACCCTGAACCCGTTGTGTCAGGTTCCGATACTAGCATTGGAAAATGACGAGATTAt                                                                                                                          <  2:548794/150‑1 (MQ=255)
                             aGCTGATGCTAACACTGGCTGATATTCCTTATCAGTTCGTTGATGTCAGTGGTTTTGACCACGAGGGAGCTTCACGCGAGTTATTGAAAACCCTGAACCCGTTGTGTCAGGTTCCGATACTGGCATTGGAAAATGACGAGATTATGACGga                                                                                                                    <  2:281359/151‑1 (MQ=255)
                               cTGATGCTAACACTGGCTGATATTCCTTATCAGTTCGTTGATGTCAGTGGTTTTGACCACGAGGGAGCTTCACGCGAGTTATTGAAAACCCTGAACCCGTTGTGTCAGGTTCCGATACTGGCATTGGAAAATGACGAGATTATGACgg                                                                                                                     >  1:706795/1‑148 (MQ=255)
                                 gATGCTAACACTGGCTGATATTCCTTATCAGTTCGTTGATGTCAGTGGTTTTGACCACGAGGGAGCTTCACGCGAGTTATTGAAAACCCTGAACCCGTTGTGTCAGGTTCCGATACTGGCATTGGAAAATGACGAGATTATGACGgaga                                                                                                                  <  1:571652/149‑1 (MQ=255)
                                  aTGCTAACACTGGCTGATATTCCTTATCAGTTCGTTGATGTCAGTGGTTTTGACCACGAGGGAGCTTCACGCGAGTTATTGAAAACCCTGAACCCGTTGTGTCAGGTTCCGATACTGGCATTGGAAAATGACGAGATTATGACGGAGAc                                                                                                                 >  1:408905/1‑149 (MQ=255)
                                     cTAACACTGGCTGATATTCCTTATCAGTTCGTTGATGTCAGTGGTTTTGACCACGAGGGAGCTTCACGCGAGTTATTGAAAACCCTGAACCCGTTGTGTCAGGTTCCGATACTGGCATTGGAAAATGACGAGATTATGACGGAGACAgcgg                                                                                                            >  2:89927/1‑151 (MQ=255)
                                       aaCACTGGCTGATATTCCTTATCAGTTCGTTGATGTCAGTGGTTTTGACCACGAGGGAGCTTCACGCGAGTTATTGAAAACCCTGAACCCGTTGTGTCAGGTTCCGATACTGGCATTGGAAAATGACGAGATTATGACGGAGACAgcggcg                                                                                                          <  1:479188/151‑1 (MQ=255)
                                             ggCTGATATTCCTTATCAGTTCGTTGATGTCAGTGGTTTTGACCACGAGGGAGCTTCACGCGAGTTATTGAAAACCCTGAACCCGTTGTGTCAGGTTCCGATACTGGCATTGGAAAATGACGAGATTATGACGGAGACAGCGGCGATTGc                                                                                                     >  2:728768/1‑150 (MQ=255)
                                               cTGATATTCCTTATCAGTTCGTTGATGTCAGTGGTTTTGACCACGAGGGAGCTTCACGCGAGTTATTGAAAACCCTGAACCCGTTGTGTCAGGTTCCGATACTGGCATTGGAAAATGACGAGATTATGACGGAGACAGCGGCGATTGCAtt                                                                                                  >  1:38404/1‑151 (MQ=255)
                                                 gATATTCCTTATCAGTTCGTTGATGTCAGTGGTTTTGACCACGAGGGAGCTTCACGCGAGTTATTGAAAACCCTGAACCCGTTGTGTCAGGTTCCGATACTGGCATTGGAAAATGACGAGATTATGACGGAGACAGCGGCGATTGCATTg                                                                                                 <  1:350547/150‑1 (MQ=255)
                                                 gATATTCCTTATCAGTTCGTTGATGTCAGTGGTTTTGACCACGAGGGAGCTTCACGCGAGTTATTGAAAACCCTGAACCCGTTGTGTCAGGTTCCGATACTGGCATTGGAAAATGACGAGATTATGACGGAGACAGCGGCGATTGCATTGa                                                                                                <  2:19362/151‑1 (MQ=255)
                                                  atatTCCTTATCAGTTCGTTGATGTCAGTGGTTTTGACCACGAGGGAGCTTCACGCGAGTTATTGAAAACCCTGAACCCGTTGTGTCAGGTTCCGATACTGGCATTGGAAAATGACGAGATTATGACGGAGACAGCGGCGATTGCATTGAt                                                                                               >  1:502309/1‑151 (MQ=255)
                                                    atTCCTTATCAGTTCGTTGATGTCAGTGGTTTTGACCACGAGGGAGCTTCACGCGAGTTATTGAAAACCCTGAACCCGTTGTGTCAGGTTCCGATACTGGCATTGGAAAATGACGAGATTATGACGGAGACAGCGGCGATTGCATTGATgg                                                                                             >  1:59988/1‑151 (MQ=255)
                                                      tCCTTATCAGTTCGTTGATGTCAGTGGTTTTGACCACGAGGGAGCTTCACGCGAGTTATTGAAAACCCTGAACCCGTTGTGTCAGGTTCCGATACTGGCATTGGAAAATGACGAGATTATGACGGAGACAGCGGCGATTGCATTGATGGtt                                                                                           >  2:250961/1‑151 (MQ=255)
                                                           aTCAGTTCGTTGATGTCAGTGGTTTTGACCACGAGGGAGCTTCACGCGAGTTATTGAAAACCCTGAACCCGTTGTGTCAGTTTCCGATACTGGCATTGGAAAATGACGAGATGATGACGGAGACAGCGGCGATTGCATTGATGGTtctc                                                                                        <  2:179806/149‑1 (MQ=255)
                                                           aTCAGTTCGTTGATGTCAGTGGTTTTGACCACGAGGGAGCTTCACGCGAGTTATTGAAAACCCTGAACCCGTTGTGTCAGGTTCCGATACTGGCATTGGAAAATGACGAGATTATGACGGAGACAGCGGCGATTGCATTGATGGTTCTCg                                                                                       >  2:732151/1‑150 (MQ=255)
                                                             cAGTTCGTTGATGTCAGTGGTTTTGACCACGAGGGAGCTTCACGCGAGTTATTGAAAACCCTGAACCCGTTGTGTCAGGTTCCGATACTGGCATTGGAAAATGACGAGATTATGACGGAGACAGCGGCGATTGCATTGATGGTTCTCGAtc                                                                                    >  1:744316/1‑151 (MQ=255)
                                                                    ttGATGTCAGTGGTTTTGACCACGAGGGAGCTTCACGCGAGTTATTGAAAACCCTGAACCCGTTGTGTCAGGTTCCGATACTGGCATTGGAAAATGACGAGATTATGACGGAGACAGCGGCGATTGCATTGATGGTTCTCGATCGTCGccc                                                                             >  2:161301/1‑151 (MQ=255)
                                                                      gATGTCAGTGGTTTTGACCACGAGGGAGCTTCACGCGAGTTATTGAAAACCCTGAACCCGTTGTGTCAGGTTCCGATACTGGCATTGGAAAATGACGAGATTATGACGGAGACAGCGGCGATTGCATTGATGGTTCTCGATCGTCGCCCg                                                                            <  1:339192/150‑1 (MQ=255)
                                                                        tGTCAGTGGTTTTGACCACGAGGGAGCTTCACGCGAGTTATTGAAAACCCTGAACCCGTTGTGTCAGGTTCCGATACTGGCATTGGAAAATGACGAGATTATGACGGAGACAGCGGCGATTGCATTGATGGTTCTCGATCGTCGCCCGGAc                                                                         >  1:182011/1‑151 (MQ=255)
                                                                        tGTCAGTGGTTTTGACCACGAGGGAGCTTCACGCGAGTTATTGAAAACCCTGAACCCGTTGTGTCAGGTTCCGATACTGGCATTGGAAAATGACGAGATTATGACGGAGACAGCGGCGATTGCATTGATGGTTCTCGATCGTCGCCCGGAc                                                                         >  1:733779/1‑151 (MQ=255)
                                                                               ggTTTTGACCACGAGGGAGCTTCACGCGAGTTATTGAAAACCCTGAACCCGTTGTGTCAGGTTCCGATACTGGCATTGGAAAATGACGAGATTATGACGGAGACAGCGGCGATTGCATTGATGGTTCTCGATCGTCGCCCGGACCTTgcgc                                                                  >  2:773921/1‑151 (MQ=255)
                                                                                gTTTTGACCACGAGGGAGCTTCACGCGAGTTATTGAAAACCCTGAACCCGTTGTGTCAGGTTCCGATACTGGCATTGGAAAATGACGAGATTATGACGGAGACAGCGGCGATTGCATTGATGGTTCTCGATCGTCGCCCGGACCTTGCGcc                                                                 >  2:668201/1‑151 (MQ=255)
                                                                                              ggAGCTTCACGCGAGTTATTGAAAACCCTGAACCCGTTGTGTCAGGTTCCGATACTGGCATTGGAAAATGACGAGATTATGACGGAGACAGCGGCGATTGCATTGATGGTTCTCGATCGTCGCCCGGACCTTGCGCCACCGGTTGGgcgcg                                                   <  1:451920/151‑1 (MQ=255)
                                                                                                 gCTTCACGCGAGTTATTGAAAACCCTGAACCCGTTGTGTCAGGTTCCGATACTGGCATTGGAAAATGACGAGATTATGACGGAGACAGCGGCGATTGCATTGATGGTTCTCGATCGTCGCCCGGACCTTGCGCCACCGGTTGGGCGCGCCg                                                <  1:178904/151‑1 (MQ=255)
                                                                                                       cgcgAGTTATTGAAAACCCTGAACCCGTTGTGTCAGGTTCCGATACTGGCATTGGAAAATGACGAGATTATGACGGAGACAGCGGCGATTGCATTGATGGTTCTCGATCGTCGCCCGGACCTTGCGCCACCGGTTGGGCGCGCCGAACGt                                           <  1:157719/150‑1 (MQ=255)
                                                                                                       cgcgAGTTATTGAAAACCCTGAACCCGTTGTGTCAGGTTCCGATACTGGCATTGGAAAATGACGAGATTATGACGGAGACAGCGGCGATTGCATTGATGGTTCTCGATCGTCGCCCGGACCTTGCGCCACCGGTTGGGCGCGCCGAACGt                                           <  1:587429/150‑1 (MQ=255)
                                                                                                            gTTATTGAAAACCCTGAACCCGTTGTGTCAGGTTCCGATACTGGCATTGGAAAATGACGAGATTATGACGGAGACAGCGGCGATTGCATTGATGGTTCTCGATCGTCGCCCGGACCTTGCGCCACCGGTTGGGCGCGCCGAACGTCAGCTg                                     >  2:161584/1‑151 (MQ=255)
                                                                                                             ttATTGAAAACCCTGAACCCGTTGTGTCAGGTTCCGATACTGGCATTGGAAAATGACGAGATTATGACGGAGACAGCGGCGATTGCATTGATGGTTCTCGATCGTCGCCCGGACCTTGCGCCACCGGTTGGGCGCGCCGAACGTCAGCTGt                                    >  1:101251/1‑151 (MQ=255)
                                                                                                               aTTGAAAACCCTGAACCCGTTGTGTCAGGTTCCGATACTGGCATTGGAAAATGACGAGATTATGACGGAGACAGCGGCGATTGCATTGATGGTTCTCGATCGTCGCCCGGACCTTGCGCCACCGGTTGGGCGCGCCGAACGTCAGCTGttt                                  >  1:628169/1‑151 (MQ=255)
                                                                                                                ttGAAAACCCTGAACCCGTTGTGTCAGGTTCCGATACTGGCATTGGAAAATGACGAGATTATGACGGAGACAGCGGCGATTGCATTGATGGTTCTCGATCGTCGCCCGGACCTTGCGCCACCGGTTGGGCGCGCCGAACGTCAGCTGTTTc                                 >  2:542948/1‑151 (MQ=255)
                                                                                                                           gAACCCGTTGTGTCAGGTTCCGATACTGGCATTGGAAAATGACGAGATTATGACGGAGACAGCGGCGATTGCATTGATGGTTCTCGATCGTCGCCCGGACCTTGCGCCACCGGTTGGGCGCGCCGAACGTCAGCTGTTTCAACGGCTAtt                       <  2:149746/150‑1 (MQ=255)
                                                                                                                            aaCCCGTTGTGTCAGGTTCCGATACTGGCATTGGAAAATGACGAGATTATGACGGAGACAGCGGCGATTGCATTGATGGTTCTCGATCGTCGCCCGGACCTTGCGCCACCGGTTGGGCGCGCCGAACGTCAGCTGTTTCAACGGCTATTgg                     >  2:5099/1‑151 (MQ=255)
                                                                                                                             aCCCGTTGTGTCAGGTTCCGATACTGGCATTGGAAAATGACGAGATTATGACGGAGACAGCGGCGATTGCATTGATGGTTCTCGATCGTCGCCCGGACCTTGCGCCACCGGTTGGGCGCGCCGAACGTCAGCTGTTTCAACGGCTATTgg                     >  1:281333/1‑150 (MQ=255)
                                                                                                                                 gTTGTGTCAGGTTCCGATACTGGCATTGGAAAATGACGAGATTATGACGGAGACAGCGGCGATTGCATTGATGGTTCTCGATCGTCGCCCGGACCTTGCGCCACCGGTTGGGCGCGCCGAACGTCAGCTGTTTCAACGGCTATTGGTctgg                >  1:33655/1‑151 (MQ=255)
                                                                                                                                    gtgtCAGGTTCCGATACTGGCATTGGAAAATGACGAGATTATGACGGAGACAGCGGCGATTGCATTGATGGTTCTCGATCGTCGCCCGGACCTTGCGCCACCGGTTGGGCGCGCCGAACGTCAGCTGTTTCAACGGCTATTGGTctggctg             <  2:727366/151‑1 (MQ=255)
                                                                                                                                         aGGTTCCGATACTGGCATTGGAAAATGACGAGATTATGACGGAGACAGCGGCGATTGCATTGATGGTTCTCGATCGTCGCCCGGACCTTGCGCCACCGGTTGGGCGCGCCGAACGTCAGCTGTTTCAACGGCTATTGGTCTGGCTGGTTGc        >  1:475626/1‑151 (MQ=255)
                                                                                                                                               cGATACTGGCATTGGAAAATGACGAGATTATGACGGAGACAGCGGCGATTGCATTGATGGTTCTCGATCGTCGCCCGGACCTTGCGCCACCGGTTGGGCGCGCCGAACGTCAGCTGTTTCAACGGCTATTGGTCTGGCTGGTTGCTAATGt  <  1:5084/151‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CGTACCCGGATGGGGCTCTACAATCAGTGAGCTGATGCTAACACTGGCTGATATTCCTTATCAGTTCGTTGATGTCAGTGGTTTTGACCACGAGGGAGCTTCACGCGAGTTATTGAAAACCCTGAACCCGTTGTGTCAGGTTCCGACACTGGCATTGGAAAATGACGAGATTATGACGGAGACAGCGGCGATTGCATTGATGGTTCTCGATCGTCGCCCGGACCTTGCGCCACCGGTTGGGCGCGCCGAACGTCAGCTGTTTCAACGGCTATTGGTCTGGCTGGTTGCTAATGT  >  NC_000913/1526264‑1526557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑