Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,582,048	A→G	G159G (GGT→GGC)	ydeN ←	putative Ser‑type periplasmic non‑aryl sulfatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,582,048	0	A	G	100.0%	212.1 / NA	56	G159G (GGT→GGC)	ydeN	putative Ser‑type periplasmic non‑aryl sulfatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/26);  total (30/26)

GTGAAGTTGTCATGATAGTCACGCGTTTGTTTATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATT  >  NC_000913/1581902‑1582180                                                                                                                                                   |                                                                                                                                     gtgAAGTTGTCATGATAGTCACGCGTTTGTTTATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCAt                                                                                                                                  <  2:537376/151‑1 (MQ=255) gtgAAGTTGTCATGATAGTCACGCGTTTGTTTATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCAt                                                                                                                                  <  2:316568/151‑1 (MQ=255)  tgAAGTTGTCATGATAGTCACGCGTTTGTTTATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCAt                                                                                                                                  >  2:482742/1‑150 (MQ=255)     aGTTGTCATGATAGTCACGCGTTTGTTTATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTg                                                                                                                              <  1:336959/151‑1 (MQ=255)         gTCATGATAGTCACGCGTTTGTTTATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGc                                                                                                                           <  1:136968/150‑1 (MQ=255)                     aCGCGTTTGTTTATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGaa                                                                                                              <  1:506811/151‑1 (MQ=255)                           ttgtttATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGac                                                                                                         >  1:546519/1‑150 (MQ=255)                            tgtttATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGaca                                                                                                        <  1:404062/150‑1 (MQ=255)                             gtttATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACAcc                                                                                                      >  2:193158/1‑151 (MQ=255)                               ttATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCa                                                                                                     <  2:507239/150‑1 (MQ=255)                                tATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCaa                                                                                                    >  1:556489/1‑150 (MQ=255)                                 aTCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCaa                                                                                                    >  2:773564/1‑149 (MQ=255)                                     tCCGGTACCGTCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACAAAAgc                                                                                                 <  1:458695/148‑1 (MQ=255)                                         gTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGcgggcggg                                                                                          >  2:476905/1‑151 (MQ=255)                                           aCCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGa                                                                                         <  2:350394/150‑1 (MQ=255)                                             cGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCt                                                                                      >  1:622840/1‑151 (MQ=255)                                               gCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCg                                                                                    <  1:334104/151‑1 (MQ=255)                                                cacaTTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGa                                                                                   <  2:354181/151‑1 (MQ=255)                                                 acaTTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGAc                                                                                  >  2:219896/1‑151 (MQ=255)                                                 acaTTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGAc                                                                                  <  2:556541/151‑1 (MQ=255)                                                    ttACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCgg                                                                               >  2:216361/1‑151 (MQ=255)                                                    ttACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCgg                                                                               >  1:492787/1‑151 (MQ=255)                                                      aCTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGt                                                                              <  1:92627/150‑1 (MQ=255)                                                      aCTGATTCTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCgg                                                                               <  2:413966/149‑1 (MQ=255)                                                           tttttGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCattat                                                                        >  2:128807/1‑151 (MQ=255)                                                              ttGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTgc                                                                     >  2:579034/1‑151 (MQ=255)                                                               tGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTgcg                                                                    >  1:328655/1‑151 (MQ=255)                                                                 aCAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTgcggc                                                                  <  2:516998/151‑1 (MQ=255)                                                                           tttACCTACTGCTGAAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACGCCAAGGCGGGCGAGAGCACGACCGGTCATTATTGCGGCGCGGGAgtg                                                         <  1:156714/150‑3 (MQ=255)                                                                               ccTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCgg                                                    <  1:743377/151‑1 (MQ=255)                                                                                  aCTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGaa                                                  >  2:42382/1‑150 (MQ=255)                                                                                    tgctgcAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAac                                                >  1:331394/1‑150 (MQ=255)                                                                                     gctgcAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAac                                                <  2:297470/149‑1 (MQ=255)                                                                                        gcAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACAcc                                             <  1:193125/149‑1 (MQ=255)                                                                                          aGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCgtgt                                         <  2:299095/151‑1 (MQ=255)                                                                                           gtgtAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGtgtg                                        >  1:472330/1‑151 (MQ=255)                                                                                               aataaCCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCcac                                    <  1:290834/151‑1 (MQ=255)                                                                                               aataaCCACGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTTGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGAAGGGGCCGGAAACACCGTTTGCca                                     <  1:216760/150‑1 (MQ=255)                                                                                                ataaCCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCcac                                    >  1:404214/1‑150 (MQ=255)                                                                                                   aCCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACAt                                  >  1:365027/1‑149 (MQ=255)                                                                                                    ccATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGc                               >  2:540713/1‑151 (MQ=255)                                                                                                     cATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGcc                              >  2:234038/1‑151 (MQ=255)                                                                                                      aTGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCg                             <  2:591114/151‑1 (MQ=255)                                                                                                        gATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGtt                           <  2:47333/151‑1 (MQ=255)                                                                                                        gATTCTGAAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGGATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGTGGCCGGAAACTCCGTGTGCCACATAGCCGtt                           <  1:713072/151‑1 (MQ=255)                                                                                                          ttCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGAcc                                                                                 >  1:685647/1‑95 (MQ=255)                                                                                                          ttCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGAcc                                                                                 <  2:685721/95‑1 (MQ=255)                                                                                                           tCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGt                        >  2:488752/1‑151 (MQ=255)                                                                                                             tGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTa                       >  1:762797/1‑150 (MQ=255)                                                                                                                  taatTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGt                 >  2:680383/1‑151 (MQ=255)                                                                                                                        aGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCt           >  2:667924/1‑151 (MQ=255)                                                                                                                          gCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCtt          >  2:504784/1‑150 (MQ=255)                                                                                                                           aaaGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTc         >  1:169147/2‑150 (MQ=255)                                                                                                                               aaaGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATcc     >  1:300191/1‑150 (MQ=255)                                                                                                                                 aGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCAtt  >  1:423382/1‑151 (MQ=255)                                                                                                                                  gTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCAtt  >  1:332001/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                     GTGAAGTTGTCATGATAGTCACGCGTTTGTTTATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATT  >  NC_000913/1581902‑1582180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑