Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,604,698	A→G	T218A (ACG→GCG)	lsrD →	autoinducer 2 import system permease protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,604,698	0	A	G	100.0%	160.0 / NA	45	T218A (ACG→GCG)	lsrD	autoinducer 2 import system permease protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/17);  total (28/17)

TTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATT  >  NC_000913/1604554‑1604844                                                                                                                                                 |                                                                                                                                                   ttCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCgggg                                                                                                                                               >  2:370868/1‑150 (MQ=255)  tCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCgg                                                                                                                                                 <  2:20330/147‑1 (MQ=255)            tctcCTCGTTTTCTGGCTCTGGCTGCATAAACCCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTg                                                                                                                                     <  1:560437/149‑1 (MQ=255)             ctcctcGTTTTCTGGCTCTGGCTGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCgg                                                                                                                                  >  1:337428/1‑151 (MQ=255)                ctcGTTTTCTGGCTCTGGCTGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCgg                                                                                                                                  >  1:298412/1‑148 (MQ=255)                 tcGTTTTCTGGCTCTGGCTGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTcgc                                                                                                                              >  1:312600/1‑151 (MQ=255)                  cGTTTTCTGGCTCTGGCTGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTcgcc                                                                                                                             >  2:328137/1‑151 (MQ=255)                  cGTTTTCTGGCTCTGGCTGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTcgcc                                                                                                                             >  1:508784/1‑151 (MQ=255)                   gTTTTCTGGCTCTGGCTGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTcgcc                                                                                                                             >  1:132559/1‑150 (MQ=255)                    ttttCTGGCTCTGGCTGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTccccgc                                                                                                                           >  2:100238/1‑151 (MQ=255)                    ttttCTGGCTCTGGCTGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGGcgccgc                                                                                                                           >  1:212307/1‑151 (MQ=255)                                ggctGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGctgctg                                                                                                                 <  1:271340/149‑1 (MQ=255)                                   tGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTa                                                                                                              >  1:225309/1‑149 (MQ=255)                                    gCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTAt                                                                                                             <  1:662749/149‑1 (MQ=255)                                     cATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGGCGCCGCTGTGCTGCTGGTAt                                                                                                             >  1:151786/1‑148 (MQ=255)                                        aaaaCCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTAt                                                                                                        <  2:391681/150‑1 (MQ=255)                                          aaCCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTAtttt                                                                                                     >  1:47757/1‑151 (MQ=255)                                                 gCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGGATCGGATTTTgggt                                                                                                 >  2:290995/1‑146 (MQ=255)                                                 gCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCCGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGtt                                                                                                 >  1:267082/1‑148 (MQ=255)                                                       cGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGt                                                                                         >  1:729564/1‑150 (MQ=255)                                                       cGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGGCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGtt                                                                                        >  1:652292/1‑151 (MQ=255)                                                        gTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGtt                                                                                        >  1:661954/1‑150 (MQ=255)                                                               gtTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGAtctc                                                                                <  1:157813/151‑1 (MQ=255)                                                                      aTTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGGGcg                                                                          >  1:707279/1‑150 (MQ=255)                                                                           gCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGttt                                                                    <  1:339277/151‑1 (MQ=255)                                                                             aaaGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGttt                                                                    >  1:149391/1‑149 (MQ=255)                                                                             aaaGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCt                                                                  <  2:158658/151‑1 (MQ=255)                                                                                    gcgcgTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGccc                                                           <  1:370821/151‑1 (MQ=255)                                                                                        gTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGcc                                                        >  1:767951/1‑150 (MQ=255)                                                                                         tGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGGCGGCGTCTGCGGGCGCCGCTGTGATGCTGGTATCGTATTTTGGTTCAGCACGTTTCGATCTCGGGGAGGCGTTTCTTATGACCGc                                                         >  1:310360/1‑148 (MQ=255)                                                                                           gcgcTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCCCGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCAt                                                      >  1:721831/1‑149 (MQ=255)                                                                                           gcgcTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCCCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGGCGTTTCTGATGCCCGCCAt                                                      >  1:328630/1‑149 (MQ=255)                                                                                                ttATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCAccgc                                                <  1:117791/150‑1 (MQ=255)                                                                                                    aGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTTGCCGCTGTGCTGCTGGCATCGGATTTTTGTTCAGCACCGTCCGTTCGCGGTGGGTCGTTTGTGGTTTCCGCCATTACCGCCctgg                                           >  1:432924/1‑151 (MQ=255)                                                                                                           ttCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTg                                     <  2:580990/150‑1 (MQ=255)                                                                                                              cAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCgg                                 >  2:32519/1‑151 (MQ=255)                                                                                                                 ttACCCGTACCTTATGTGCGCTCTATGCCAGGGCGGGGTTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTGGGGGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCgg                                 <  1:707878/148‑1 (MQ=255)                                                                                                                              aTGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTAtgg                  >  2:643606/1‑150 (MQ=255)                                                                                                                               tgtgCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTAtggt                 <  2:538736/150‑1 (MQ=255)                                                                                                                                gtgCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGTTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTAtggt                 <  1:556593/149‑1 (MQ=255)                                                                                                                                gtgCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTAtggtgg               >  2:396117/1‑151 (MQ=255)                                                                                                                                    gcTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTggttcc           >  2:290714/1‑151 (MQ=255)                                                                                                                                         aTGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTggttccggtt       <  2:58626/150‑1 (MQ=255)                                                                                                                                            ccATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTTTTTTGGTCCAGCACGTTCCGATCTCGGTGCGTCTTTTTTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTggttccggttc      <  1:307260/148‑1 (MQ=255)                                                                                                                                             cATGGCGGGTCTGGTGTCTGCGGCCGCCGCTGTGCTGCTGGTTTCGTATTTTGGTTCAGCACGTTCCGTTCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCAtt  <  2:355272/151‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                   TTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATT  >  NC_000913/1604554‑1604844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑