Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 170,771 G→A R66H (CGC→CAC)  fhuD → iron(3+)‑hydroxamate import ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913170,7710GA97.9% 161.5 / ‑4.4 49R66H (CGC→CAC) fhuDiron(3+)‑hydroxamate import ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (26/21);  total (27/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

GCGCTTTCTCCGTTGTTATGGCAGATGAATACCGCCCACGCGGCGGCTATTGATCCCAATCGTATTGTGGCGCTGGAGTGGTTGCCGGTGGAATTACTGCTGGCGCTCGGCATCGTGCCTTACGGCGTGGCGGATACCATCAACTATCGCCTGTGGGTCAGCGAACCACCATTGCCGGACTCAGTGATCGACGTCGGTTTGCGCACAGAACCTAACCTTGAACTGCTGACCGAAATGAAACCATCGTTTATGGTCTGGTCGGCAGGATATGGCCCTTCACCAGAAATGCTGGCTCGT  >  NC_000913/170623‑170919
                                                                                                                                                    |                                                                                                                                                    
gcgcTTTCTCCGTTGTTATGGCAGATGAATACCGCCCACGCGGCGGCTATTGATCCCAATCGTATTGTGGCGCTGGAGTGGTTGCCGGTGGAATTACTGCTGGCGCTCGGCATCGTGCCTTACGGCGTGGCGGATACCATCAACTATCAcc                                                                                                                                                    >  2:361058/1‑151 (MQ=255)
    tttCTCCGTTGTTATGGCAGATGAATACCGCCCACGCGGCGGCTATTGATCCCAATCGTATTGTGGCGCTGGAGTGGTTGCCGGTGGAATTACTGCTGGCGCTCGGCATCGTGCCTTACGGCGTGGCGGACACCATCAACTATCACCtgtg                                                                                                                                                >  1:604950/1‑151 (MQ=255)
                       gATGAATACCGCCCACGCGGCGGCTCTTGATCCCAATCGTATTGTGGCGCTGGAGTGGTTGCCGGTGGAATTACTGCTGGCGCTCGGCATCGTGCCTTACGGCGTGGCGGGTACCATCCACTATTACCTGTGGGTCAGCGGACCACCAtt                                                                                                                              >  2:572996/1‑150 (MQ=255)
                           aaTACCGCCCACGCGGCGGCTATTGATCCCAATCGTATTGTGGCGCTGGAGTGGTTGCCGGTGGAATTACTGCTGGCGCTCGGCATCGTGCCTTACGGCGTGGCGGATACCATCAACTATCACCTGTGGGTCAGCGAACCACCATTGCCgg                                                                                                                         >  2:55381/1‑151 (MQ=255)
                              aCCGCCCACGCGGCGGCTATTGATCCCAATCGTATTGTGGCGCTGGAGTGGTTGCCGGTGGAATTACTGCTGGCGCTCGGCATCGTGCCTTACGGCGTGGCGGATACCATCAACTATCACCTGTGGGTCAGCGAACCACCATTGCCGGACt                                                                                                                      >  2:78511/1‑151 (MQ=255)
                                   ccACTCGGCGGCTATTGATCCCAATCGTATTGTGGCGCTGGAGTGGTTGCCGGTGGAATTACTGCTGGCGCTCGGCATCGTGCCTTACGGCGTGGCGGATACCATTAACTATCACCTGTGGGTCCGCGCACCACCCTTGTCGGACTCAg                                                                                                                   >  1:575888/1‑149 (MQ=255)
                                   ccACGCGGCGGCTATTGATCCCAATCGTATTGTGGCGCTGGAGTGGTTGCCGGTGGAATTACTGCTGGCGCTCGGCATCGTGCCTTACGGCGTGGCGGATACCATCAACTATCACCTGTGGGTCAGCGAACCACCATTGCCGGACTCAGTg                                                                                                                 <  1:333944/151‑1 (MQ=255)
                                      cgcgGCGGCTATTGATCCCAATCGTATTGTGGCGCTGGAGTGGTTGCCGGTGGAATTACTGCTGGCGCTCGGCATCGTGCCTTACGGCGTGGCGGATACCATCAACTATCACCTGTGGGTCAGCGAACCACCATTGCCGGACTCAGTGATc                                                                                                              <  1:448544/151‑1 (MQ=255)
                                       gcggcgGCTATTGATCCCAATCGTATTGTGGCGCTGGAGTGGTTGCCGGTGGAATTACTGCTGGCGCTCGGCATCGTGCCTTACGGCGTGGCGGGTACCCTCAAATATCACCCGTGGGGCAGCGAACCACCCATTCCGGACCCAGTGATCg                                                                                                             >  1:752108/1‑151 (MQ=255)
                                         ggcggcTATTGATCCCAATCGTATTGTGGCGCTGGAGTGGTTGCCGGTGGAATTACTGCTGGCGCTCGGCATCGTGCCTTACGGCGTGGCGGATACCATCAACTATCACCTGTGGGTCAGCGAac                                                                                                                                     <  1:50072/125‑1 (MQ=255)
                                         ggcggcTATTGATCCCAATCGTATTGTGGCGCTGGAGTGGTTGCCGGTGGAATTACTGCTGGCGCTCGGCATCGTGCCTTACGGCGTGGCGGATACCATCAACTATCACCTGTGGGTCAGCGAac                                                                                                                                     >  2:50103/1‑125 (MQ=255)
                                         ggcggcTATTGATCCCAATCGTATTGTGGCGCTGGAGTGGTTGCCGGTGGAATTACTGCTGGCGCTCGGCATCGTGCCTTACGGCGTGGCGGATACCATCAACTATCACCTGTGGGTCAGCGAACCCCCATTGCCGGACTCAGTGAACGa                                                                                                            >  2:218683/1‑150 (MQ=255)
                                            ggcTATTGATCCCAATCGTATTGTGGCGCTGGAGTGGTTGCCGGTGGAATTACTGCTGGCGCTCGGCATCGTGCCTTACGGCGTGGCGGATACCATCAACTATCACCGGTGGGGCAGCGAACCACCATTGCCGGACTCAGTGATCGACGTc                                                                                                        >  1:412820/1‑151 (MQ=255)
                                                aTTGATCCCAATCGTATTGTGGCGCTGGAGTGGTTGCCGGTGGAATTACTGCTGGCGCTCGGCATCGTGCCTTACGGCGTGGCGGATACCATCAACTATCACCTGTGGGTCAGCGAACCACCATTGCCGGACTCAGTGATCGACGTCGGtt                                                                                                    >  2:437578/1‑151 (MQ=255)
                                                     tCCCAATCGTATTGTGGCGCTGGAGTGGTTGCCGGTGGAATTACTGCTGGCGCTCGGCATCGTGCCTTACGGCGTGGCGGATACCATCCACTATCACCTGTGGGGCAGCGAACCACCATTGCCGGACTCAGTGATCGACGTCgg                                                                                                      >  1:743418/1‑144 (MQ=255)
                                                      cccAATCGTATTGTGGCGCTGGAGTGGTTGCCGGTGGAATTACTGCTGGCGCTCGGCATCGTGCCTTACGGCGTGGCGGATACCATCAACTATCACCTGTGGGTCAGCGAACCACCATTGCCGGACTCAGTGATCGACGTCGGTTTGCGca                                                                                              <  2:571104/151‑1 (MQ=255)
                                                            cGTATTGTGGCGCTGGAGTGGTTGCCGGTGGAATTACTGCTGGCGCTCGGCATCGTGCCTTACGGCGTGGCGGATACCATCAACTATCACCTGTGGGTCAGCGAACCACCATTGCCGGACTCAGTGATCGACGTCGGTTTGCGcac                                                                                             <  1:653037/146‑1 (MQ=255)
                                                                 tgtgGCGCTGGAGTGGTTGCCGGTGGAATTACTGCTGGCGCTCGGCATCGTGCCTTACGGCGTGGCGGATACCATCAACTATCTCCTGTGGGTCAGCGAACCACCATTGCCGGACTCAGTGATCGACGTCGGTTTGCGCACAGaacctaa                                                                                    <  1:133082/150‑1 (MQ=255)
                                                                       gcTGGAGTGGTTGCCGGTGGAATTACTGCTGGCGCTCGGCATCGTGCCTTACGGCGTGGCGGATACCATCAACTATCACCTGTGGGTCAGCGAACCACCATTGCCGGACTCAGTGATCGACGTCGGTTTGCGCACAGAACCTAACCTTGa                                                                              <  1:760395/150‑1 (MQ=255)
                                                                          ggAGTGGTTGCCGGTGGAATTACTGCTGGCGCTCGGCATCGTGCCTTACGGCGTGGCGGATACCATCAACTATCACCTGTGGGTCAGCGAACCACCATTGCCGGACTCAGTGATCGACGTCGGTTTGCGCACAGAACCTAACCTTGAAct                                                                           <  1:65143/150‑1 (MQ=255)
                                                                               ggTTGCCGGTGGAATTACTGCTGGCGCTCGGCATCGTGCCTTACGGCGTGGCGGATACCATCAACTATCACCTGTGGGTCAGCGAACCACCATTTCCGGACTCAGTGATCGACGTCGGTTTGCGCACAGAACCTAACCTTGAACTGCTGAc                                                                     >  1:593761/1‑151 (MQ=255)
                                                                               ggTTGCCGGTGGAATTACTGCTGGCGCTCGGCATCGTGCCTTACGGCGTGGCGGATACCATCAACTATCACCTGTGGGTCAGCGAACCACCATTGCCGGACTCAGTGATCGACGTCGGTTTGCGCACAGAACCTAACCTTGAACTGCTGAc                                                                     <  2:712157/151‑1 (MQ=255)
                                                                                        tGGAATTACTGCTGGCGCTCGGCATCGTGCCTTACGGCGTGGCGGATACCATCAACTATCACCTGTGGGTCAGCGAACCACCATTGCCGGACTCAGTGATCGACGTCGGGTTGCGCACAGAACCTAACCTTGAACTGCTGACCGaaa                                                                >  1:290256/1‑147 (MQ=255)
                                                                                         ggAATTACTGCTGGCGCTCGGCATCGTGCCTTACGGCGTGGCGGATACCATCAACTATCACCTGTGGGTCAGCGAACCACCATTGCCGGACTCAGTGATCGACGTCGGTTTGCGCACAGAACCTAACCTTGAACTGCTGACCGAAATGaa                                                            <  2:446709/150‑1 (MQ=255)
                                                                                          gAATTACTGCTGGCGCTCGGCATCGTGCCTTACGGCGTGGCGGATACCATCAACTATCACCTGTGGGTCAGCGAACCACCATTGCCGGACTCAGTGATCGACGTCGGTTTGCGCACAGAACCTAACCTTGAACTGCTGACCGAAATGAAAc                                                          >  1:666132/1‑151 (MQ=255)
                                                                                          gAATTACTGCTGGCGCTCGGCATCGTGCCTTACGGCGTGGCGGATACCATCAACTATCACCTGTGGGTCAGCGAACCACCATTGCCGGACTCAGTGATCGACGTCGGTTTGCGCACAGAACCTAACCTTGAACTGCTGACCGAAATGAAAc                                                          <  2:562658/151‑1 (MQ=255)
                                                                                             ttACTGCTGGCGCTCGGCATCGTGCCTTACGGCGCGGCGGACAACCTCAACTCTCACCTGCGGGACAGCGAACCAACATTGCCGGACTCAGAGATCGCCGTGGGGTTGGGCACAGAACCTAACCTTGAACGGCTGGCCGAAATGAAAcc                                                         >  1:230331/1‑149 (MQ=255)
                                                                                              tACTGCTGGCGCTCGGCATCGTGCCTTACGGCGTGGCGGATACCATCAACTATCACCTGTGGGTCAGCGAACCACCATTGCCGGACTCAGTGATCGACGTCGGTTTGCGCACAGAACCTAACCTTGAACTGCTGACCGAAATGAAACCAt                                                       <  1:719754/150‑1 (MQ=255)
                                                                                                     ggCGCTCGGCATCGTGCCTTACGGCGTGGCGGATACCATCAACTATCACCTGTGGGTCAGCGAACCACCATTGCCGGACTCAGTGATCGACGTCGGTTTGCGCACAGAACCTAACCTTGAACTGCTGACCGAAATGAAACCATCGTTTAtg                                               <  2:491696/151‑1 (MQ=255)
                                                                                                     ggCGCTCGGCATCGTGCCTTACGGCGTGGCGGATACCATCAACTATCACCTGTGGGTCAGCGAACCACCATTGCCGGACTCAGTGATCGACGTCGGTTTGCGCACAGAACCTAACCTTGAACTGCTGACCGAAATGAAACCATCGTTTAt                                                <  2:564823/150‑1 (MQ=255)
                                                                                                            ggCATCGTGCCTTACGGCGTGGCGGATACCATCAACTATCACCTGTGGGTCAGCGAACCACCATTGCCGGACTCAGTGATCGACGTCGGTTTGCGCACAGAACCTAACCTTGAACTGCTGACCGAAATGAAACCATCGTTTAtggtctgg                                         >  1:235850/1‑150 (MQ=255)
                                                                                                               aTCGTGCCTTACGGCGTGGCGGATACCATCAACTATCACCTGTGGGTCAGCGAACCACCATTGCCGGACTCAGTGATCGACGTCGGTTTGCGCACAGAACCTAACCTTGAAc                                                                            <  2:521792/112‑1 (MQ=255)
                                                                                                               aTCGTGCCTTACGGCGTGGCGGATACCATCAACTATCACCTGTGGGTCAGCGAACCACCATTGCCGGACTCAGTGATCGACGTCGGGTTGCGCACAGAACCTAACCTTGaa                                                                             >  1:521740/1‑111 (MQ=255)
                                                                                                                  gTGCCTTACGGCGTGGCGGATACCATCAACTATCACCTGTGGGTCAGCGAACCACCATTGCCGGACTCAGTGATCGACGTCGGTTTGCGCACAGAACCTAACCTTGAACTGCTGACCGAAATGAAACCATCGTTTATGGTCTGGTCgg                                     >  2:516770/1‑148 (MQ=255)
                                                                                                                        tACGGCGTGGCGGATACCATCAACTATCACCTGTGGGTCAGCGAAACACCATTGCCGGACTCAGTGATCGACGGCGGTTTGCGCACAGAACCTAACCTTGAACTGCTGACCGAAATGAAACCATCGTTTATGGTCTGGGCGGCAGGata                              >  1:775194/1‑149 (MQ=255)
                                                                                                                         aCGGCGTGGCGGATACCATCAACTATCACCTGTGGGTCAGCGAACCACCATTGCCGGACTCAGTGATCGACGTCGGTTTGCGCACAGAACCTAACCTTGAACTGCTGACCGAAATGAAACCATCGTTTATGGTCTGGTCGGCAGGATATgg                           >  2:229721/1‑151 (MQ=255)
                                                                                                                              gTGGCGGATACCATCAACTATCACCTGTGGGTCAGCGAACCACCATTGCCGGACTCAGTGATCGACGTCGGTTTGCGCACAGAGCCTAACCTTGAACTGCTGACCGAAATGAAACCATCGTTTATGGTCTGGTCGGCAGGATATGGCCCtt                      <  1:658700/151‑1 (MQ=255)
                                                                                                                              gTGGCGGATACCATCAACTATCACCTGTGGGTCAGCGAACCACCATTGCCGGACTCAGTGATCGACGTCGGTTTGCGCACAGAACCTAACCTTGAACTGCTGACCGAAATGAAACCATCGTTTATGGTCTGGTCGGCAGGATATGGCCCtt                      <  1:268873/151‑1 (MQ=255)
                                                                                                                              gTGGCGGATACCATCAACTATCACCTGTGGGTCAGCGAACCACCATTGCCGGACTCAGTGATCGACGTCGGTTTGCGCACAGAACCTAACCTTGAACTGCTGACCGAAATGAAACCATCGTTTATGGTCTGGTCGGCAGGATATGGCCCtt                      <  1:623305/151‑1 (MQ=255)
                                                                                                                              gTGGCGGATACCATCAACTATCACCTGTGGGTCAGCGAACCACCATTGCCGGACTCAGTGATCGACGTCGGTTTGCGCACAGAACCTAACCTTGAACTGCTGACCGAAATGAAACCATCGTTTATGGTCTGGTCGGCAGGATATGGCCCtt                      <  1:553271/151‑1 (MQ=255)
                                                                                                                                ggCGGATACCATCAACTATCACCTGTGGGTCAGCGAACCACCATTGCCGGACTCAGTGATCGACGTCGGTTTGCGCTCAGAACCTAACCTTGAACTGCTGACCGAAATGAAACCATCGTTTATGGTCTGGTCGGCAGGATATGGCCCTTCa                    <  1:673062/151‑1 (MQ=255)
                                                                                                                                  cGGATACCATCAACTATCACCTGTGGGTCAGCGAACCACCATTGCCGGACTCAGTGATCGACGTCGGTTTGCGCACAGAACCTAACCTTGAACTGCTGACCGAAATGAAACCATCGTTTATGGTCTGGTCGGCAGGATATGGCCCTTCAcc                  >  2:522459/1‑151 (MQ=255)
                                                                                                                                    gATACCATCAACTATCACCTGTGGGTCAGCGAACCACCATTGCCGGACTCAGTGATCGACGTCGGTTTGCGCACAGAACCTAACCTTGAACTGCTGACCGAAATGAAACCATCGTTTATGGTCTGGTCGGCAGGATATGGCCCTTCAcc                  >  1:206445/1‑149 (MQ=255)
                                                                                                                                     aTACCATCAACTATCACCTGTGGGTCAGCGAACCACCATTGCCGGACTCAGTGATCGACGTCGGTTTGCGCACAGAACCTAACCTTGAACTGCTGACCGAAATGAAACCATCGTTTATGGTCTGGTCGGCAGGATATGGCCCTTCACCAGa               <  2:396028/151‑1 (MQ=255)
                                                                                                                                      tACCATCAACTATCACCTGTGGGTCAGCGAACCACCATTGCCGGACTCAGTGATCGACGTCGGTTTGCGCACAGAACCTAACCTTGATCTGCTGACCGAAATGAAACCATCGTTTATGGTCTGGTCGGCAGGATATGGCCCTTCACCAGa               <  2:663297/150‑1 (MQ=255)
                                                                                                                                        ccATCAACTATCACCTGTGGGTCAGCGAACCACCATTGCCGGACTCAGTGATCGACGTCGGTTTGCGCACAGAACCTAACCTTGAACTGCTGACCGAAATGAAACCATCGTTTATGGTCTGGTCGGCAGGATATGGCCCTTCACCAGAAAt            >  1:655456/1‑151 (MQ=255)
                                                                                                                                               cTATCACCTGTGGGTCAGCGAACCACCATTGCCGGACTCAGTGATCGACGTCGGTTTGCGCACAGAACCTAACCTTGAACTGCTGACCGAAATGAAACCATCGTTTATGGTCTGGTCGGCAGGATATGGCCCTTCACCAGAAATGCTGGc      >  2:547628/1‑150 (MQ=255)
                                                                                                                                                tATCACCTGTGGGTCAGCGAACCACCATTGCCGGACTCAGTGATCGACGTCGGTTTGCGCACAGAACCTAACCTTGAACTGCTGACCGAAATGAAACCATCGTTTATGGTCTGGTCGGCAGGATATGGCCCTTCACCAGAAATGCTGGCTc    >  2:142400/1‑151 (MQ=255)
                                                                                                                                                   cGCCTGTGGGTCAGCGAACCACCATTGCCGGACTCAGTGATCGACGTCGGGTTGCGCACAGAACCTAACCTTGAACTGCTGACCGAAATGAAACCATCGTTTATGGTCTGGTCGGCAGGATATGGCCCTTCACCAGAAATGCTGGCTCGt  >  2:21747/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCGCTTTCTCCGTTGTTATGGCAGATGAATACCGCCCACGCGGCGGCTATTGATCCCAATCGTATTGTGGCGCTGGAGTGGTTGCCGGTGGAATTACTGCTGGCGCTCGGCATCGTGCCTTACGGCGTGGCGGATACCATCAACTATCGCCTGTGGGTCAGCGAACCACCATTGCCGGACTCAGTGATCGACGTCGGTTTGCGCACAGAACCTAACCTTGAACTGCTGACCGAAATGAAACCATCGTTTATGGTCTGGTCGGCAGGATATGGCCCTTCACCAGAAATGCTGGCTCGT  >  NC_000913/170623‑170919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑