| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,608,413 | T→C | T204A (ACG→GCG) | yneE ← | bestrophin family putative inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,608,413 | 0 | T | C | 98.0% | 184.9 / NA | 51 | T204A (ACG→GCG) | yneE | bestrophin family putative inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (25/25); total (25/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTCATGTAATGCAGGTCCACGACCAGCGCGAACGGCAGCATGATACAAAACAGATAAACGGTACGATGCAAAATCAGGGTGTAGGCAAAGGGAATTGGCGTATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCGCTGAACCGCCAACCATTCTCCCATTATTAACAAGATACGG > NC_000913/1608265‑1608552 | cAATCCAGCGAAATAAAAGTGTAAGAAATCAGCACAGAGATAAAAGGCGTCATGTAATGCAGGTCCACGACCAGCGCGAACGGCAGCATGATACAACACAGATAAACCGTACGATGCAAACTCAGGGTCTAGGCCAAGGGAATTGGCGtt < 1:600957/150‑2 (MQ=255) aaaTAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTCATGTAATGCAGGTCCACGACCAGCGCGAACGGCAGCATGATACAAAACAGATAAACGGTACGATGCAAAATCAGGGTGTAGGCAAAGGGAATTGGCGCATAGGCAATgcg > 1:721243/1‑151 (MQ=255) aTAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTCATGTAATGCAGGTCCACGACCAGCGCGAACGGCAGCATGATACAAAACAGATAAACGGTACGATGCAAAATCAGGGTGTAGGCAAAGGGAATTGGCGCATAGGCAATgagc > 1:349386/1‑150 (MQ=255) aTAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTCATGTAATGCAGGTCCACGACCAGCGCGAACGGCAGCATGATACAAAACAGATAAACGGTACGATGCAAAATCAGGGTGTAGGCAAAGGGAATTGGCGCATAGGCAATGCGCt > 2:500185/1‑151 (MQ=255) tgtAGGAAATCAGCACAGAGATAAAAGGCGTCATGTAATGCAGGTCCACGACCAGCGCGAACGGCAGCATGATACAAAACAGATAAACGGTACGATGCAAAATCAGGGTGTAGGCAAAGGGAATTGGCGCATAGGCAATGCGCTCGCATcc > 2:579430/1‑151 (MQ=255) gtAGGAAATCAGCACAGAGATAAAAGGCGTCATGTAATGCAGGTCCACGACCAGCGCGAACGGCAGCATGATACAAAACAGATAAACGGTACGATGCAAAATCAGGGTGTAGGCAAAGGGAATTGGCGCATAGGCAATGCGCTCGCATCCt < 1:154233/151‑1 (MQ=255) tAGGAAATCAGCACAGAGATAAAAGGCGTCATGTAATGCAGGTCCACGACCAGCGCGAACGGCAGCATGATACAAAACAGATAAACGGTACGATGCAAAATCAGGGTGTAGGCAAAGGGAATTGGCGCATAGGCAATGCGCTCGCATCCTg > 1:299110/1‑151 (MQ=255) gAAATCAGCACAGAGATAAAAGGCGTCATGTAATGCAGGTCCACGACCAGCGCGAACGGCAGCATGATACAAAACAGATAAACGGTACGATGCAAAATCAGGGTGTAGGCAAAGGGAATTGGCGCATAGGCAATGCGCTCGCATCCTGCCa < 1:279583/151‑1 (MQ=255) gAAATCAGCACAGAGATAAAAGGCGTCATGTAATGCAGGCCCACGACCAGCGCGAACGGCAGCATGATACAAAACAGATAAACGGTACGATGCAAAATCAGGGTGTAGGCAAAGGGAATTGGCGCATAGGCAATGCGCTCGCATCCTGCCa < 2:349673/151‑1 (MQ=255) aaTCAGCACAGAGATAAAAGGCGTCATGTAATGCAGGTCCACGACCAGCGCGAACGGCAGCATGATACAAAACAGATAAACGGTACGATGCAAAATCAGGGTGTAGGCAAAGGGAATTGGCGCATAGGCAATGCGCTCGCATCCTGCCAgg < 2:151479/151‑1 (MQ=255) cAGCACAGAGATAAAAGGCGTCATGTAATGCAGGTCCACGACCAGCGCGAACGGCAGCATGATACAAAACAGATAAACGGTACGATGCAAAATCAGGGTGTAGGCAAAGGGAATTGGCGCATAGGCAATGCGCTCGCATCCTGCCAGGAcc < 1:500133/151‑1 (MQ=255) cAGCACAGAGATAAAAGGCGTCATGTAATGCAGGTCCACGACCAGCGCGAACGGCAGCATGATACAAAACAGATAAACGGTACGATGCAAAATCAGGGTGTAGGCAAAGGGAATTGGCGCATAGGCAATGCGCTCGCATCCTGCCAGGAc < 2:216999/150‑1 (MQ=255) aGCACAGAGATAAAAGGCGTCATGTAATGCAGGTCCACGACCAGCGCGAACGGCAGCATGATACAAAACAGATAAACGGTACGATGCAAAATCAGGGTGTAGGCAAAGGGAATTGGCGCATAGGCAATGCGCTCGCATCCTGCCAGGACCg > 1:397806/1‑151 (MQ=255) cacaGAGATAAAAGGCGTCATGTAATGCAGGTCCACGACCAGCGCGAACGGCAGCATGATACAAAACAGATAAACGGTACGATGCAAAATCAGGGTGTAGGCAAAGGGAATTGGCGCATAGGCAATGCGCTCGCATCCTGCCAGGACCGCt < 1:63995/151‑1 (MQ=255) agagATAAAAGGCGTCATGTAATGCAGGTCCACGACCAGCGCGAACGGCAGCATGATACAAAACAGATAAACGGTACGATGCAAAATCAGGGTGTAGGCAAAGGGAATTGGCGCATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGa > 2:414642/1‑150 (MQ=255) aaGGCGTCATGTAATGCAGGTCCACGACCAGCGCGAACGGCAGCATGATACAAAACAGATAAACGGTACGATGCAAAATCAGGGTGTAGGCAAAGGGAATTGGCGCATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCAtt < 2:387908/151‑1 (MQ=255) gTCATGTAATGCAGGTCCACGACCAGCGCGAACGGCAGCATGATACAAAACAGATAAACGGTACGATGCAAAATCAGGGTGTAGGCAAAGGGAATTGGCGCATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGcc < 2:390052/151‑1 (MQ=255) cATGTAATGCAGGTCCACGACCAGCGCGAACGGCAGCATGATACAAAACAGATAAACGGTACGATGCAAAATCAGGGTGTAGGCAAAGGGAATTGGCGCATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCg < 2:296392/150‑1 (MQ=255) aaTGCAGGTCCACGACCAGCGCGAACGGCAGCATGATACAAAACAGATAAACGGTACGATGCAAAATCAGGGTGTAGGCAAAGGGAATTGGCGCATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGtt > 1:348016/1‑151 (MQ=255) aaTGCAGGTCCACGACCAGCGCGAACGGCAGCATGATACAAAACAGATAAACGGTACGATGCAAAATCAGGGTGTAGGCAAAGGGAATTGGCGCATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGtt < 1:378294/151‑1 (MQ=255) gCAGGTCCACGACCAGCGCGAACGGCAGCATGATACAAAACAGATAAACGGTACGATGCAAAATCAGGGTGTAGGCAAAGGGAATTGGCGCATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTg > 1:200376/1‑149 (MQ=255) aGGTCCACGACCAGCGCGAACGGCAGCATGATACAAAACAGATAAACGGTACGATGCAAAATCAGGGTGTAGGCAAAGGGAATTGGCGCATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGc < 1:647070/151‑1 (MQ=255) ggTCCACGACCAGCGCGAACGGCAGCATGATACAAAACAGATAAACGGTACGATGCAAAATCAGGGTGTAGGCAAAGGGAATTGGCGCATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCt < 1:252512/151‑1 (MQ=255) cGACCAGCGCGAACGGCAGCATGATACAAAACAGATAAACGGTACGATGCAAAATCAGGGTGTAGGCAAAGGGAATTGGCGCATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATaaa < 2:758434/151‑1 (MQ=255) gACCAGCGCGAACGGCAGCATGATACAAAACAGATAAACGGTACGATGCAAAATCAGGGTGTAGGCAAAGGGAATTGGCGCATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAAc > 1:719805/1‑151 (MQ=255) cAGCGCGAACGGCAGCATGATACAAAACAGATAAACGGTACGATGCAAAATCAGGGTGTAGGCAAAGGGAATTGTCGCATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAgg < 1:581635/151‑1 (MQ=255) cgcgAACGGCAGCATGATACAAAACAGATAAACGGTACGATGCAAAATCAGGGCGTAGGCAAAGGGAATTGGCGCATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGat > 1:64942/1‑150 (MQ=255) gcagcaTGATACAAAACAGATAAACGGTACGATGCAAAATCAGGGTGTAGGCAAAGGGAATTGGCGCATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAg < 2:23727/151‑1 (MQ=255) caTGATACAAAACAGATAAACGGTACGATGCAAAATCAGGGTGTAGGCAAAGGGAATTGGCGCATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGt > 1:687077/1‑151 (MQ=255) gATACAAAACAGATAAACGGTACGATGCAAAATCAGGGTGTAGGCAAAGGGAATTGGCGCATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTcc > 1:270676/1‑150 (MQ=255) gATACAAAACAGATAAACGGTACGATGCAAAATCAGGGTGTAGGCAAAGGGAATTGGCGCATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCa < 1:498644/151‑1 (MQ=255) aTACAAAACAGATAAACGGTACGATGCAAAATCAGGGTGTAGGCAAAGGGAATTGGCGCATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCAt < 1:309114/151‑1 (MQ=255) aGATAAACGGTACGATGCAAAATCAGGGTGTAGGCAAAGGGAATTGGCGCATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCGCt < 1:273976/151‑1 (MQ=255) gATAAACGGTACGATGCAAAATCAGGGTGTAGGCAAAGGGAATTGGCGCATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCGCTg < 1:296099/151‑1 (MQ=255) aaaCGGTACGATGCAAAATCAGGGTGTAGGCAAAAGGAATTGGCGCATAGGCAATGCACTCGCATCCTGCCAGGACCGCTAAAATATAATTAAGCCGCTCGTTGAGGCTAATAAACAGGATATCTGAAACCTGTCCATTGCGGCACTGa > 1:440501/1‑149 (MQ=255) aaCGGTACGATGCAAAATCAGGGTGTAGGCAAAGGGAATTGGCGCATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCGCTGAAcc > 2:514061/1‑151 (MQ=255) aCGGTACGATGCAAAATCAGGGTGTAGGCAAAGGGAATTGGCGCATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCGCTGAACCg > 1:220140/1‑151 (MQ=255) aCGGTACGATGCAAAATCAGGGTGTAGGCAAAGGGAATTGGCGCATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCGCTGAACCg < 1:497215/151‑1 (MQ=255) ggTACGATGCAAAATCAGGGTGTAGGCAAAGGGAATTGGCGCATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCGCTGAACCGcc > 2:404875/1‑151 (MQ=255) aCGATGCAAAATCAGGGTGTAGGCAAAGGGAATTGGCGCATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCGCTGAACCGCCAAc > 1:98357/1‑151 (MQ=255) aTGCAAAATCAGGGTGTAGGCAAAGGGAATTGGCGCATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCGCTGAACCGCCAACCAt < 2:391426/151‑1 (MQ=255) cAAAATCAGGGTGTAGGCAAAGGGAATTGGCGCATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCGCTGAACCGCCAACCATtct > 1:648723/1‑151 (MQ=255) aaaaTCAGGGTGTAGGCAAAGGGAATTGGCGCATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCGCTGAACCGCCAACCATtctc > 1:704986/1‑151 (MQ=255) aaTCAGGGTGTAGGCAAAGGGAATTGGCGCATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCGCTGAACCGCCAACCATTCTccc > 2:421132/1‑151 (MQ=255) aaTCAGGGTGTAGGCAAAGGGAATTGGCGCATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCGCTGAACCGCCAACCATTCTccc < 2:576343/151‑1 (MQ=255) tCAGGGTGTAGGCAAAGGGAATTGGCGCATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACTGGATATCTGAAAGCTGTCCATTGCGGCGCTGAACCGCCAACCATTCTCCCat > 2:607407/1‑151 (MQ=255) aGGGTGTAGGCAAAGGGAATTGGCGCATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCGCTGAACCGCCAACCATTCTCCCatta < 2:242862/151‑1 (MQ=255) aGGGTGTAGGCAAAGGGAATTGGCGCATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCGCTGAACCGCCAACCATTCTCCCatt > 1:306415/1‑150 (MQ=255) gggTGTAGGAAAAGGGAATTGGCGTGTAGGCTATGCGCCCGCATCCTGACAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGCCCAATAAACACGATATCTCAAAGCTGGCCATTGCGCCATTGACTCACCACCCATTCGcccgatat < 1:52890/151‑6 (MQ=255) aGGCAAAGGGAATTGGCGCATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCGCTGAACCGCCAACCATTCTCCCATTATTAACa > 1:327814/1‑150 (MQ=255) aaaGGGAATTGGCGCATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCGCTGAACCGCCAACCATTCTCCCATTATTAACAAGAt > 2:619563/1‑150 (MQ=255) gggAATTGGCGCATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCGCTGAACCGCCAACCATTCTCCCATTATTAACAAGATACgg < 2:538670/151‑1 (MQ=255) | CAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTCATGTAATGCAGGTCCACGACCAGCGCGAACGGCAGCATGATACAAAACAGATAAACGGTACGATGCAAAATCAGGGTGTAGGCAAAGGGAATTGGCGTATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCGCTGAACCGCCAACCATTCTCCCATTATTAACAAGATACGG > NC_000913/1608265‑1608552 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |