Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,621,321	C→T	intergenic (‑184/‑11)	eamA ← / → ydeE	cysteine and O‑acetyl‑L‑serine efflux system/putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,621,321	0	C	T	100.0%	174.1 / NA	46	intergenic (‑184/‑11)	eamA/ydeE	cysteine and O‑acetyl‑L‑serine efflux system/putative transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/19);  total (27/19)

AGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCCGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATC  >  NC_000913/1621179‑1621466                                                                                                                                               |                                                                                                                                                  aGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGgacagac                                                                                                                                           <  2:52043/151‑1 (MQ=255)     gAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAAt                                                                                                                                       >  2:139209/1‑151 (MQ=255)         ttACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGaa                                                                                                                                    >  2:162039/1‑150 (MQ=255)           aCTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACtt                                                                                                                                 <  2:17477/151‑1 (MQ=255)           aCTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACt                                                                                                                                  <  2:209460/150‑1 (MQ=255)             ttttCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTAt                                                                                                                               >  1:684952/1‑151 (MQ=255)             ttttCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTAt                                                                                                                               >  2:308933/1‑151 (MQ=255)                   ttAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCt                                                                                                                           <  2:197275/149‑1 (MQ=255)                    tAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTacg                                                                                                                        >  1:252157/1‑151 (MQ=255)                      aCAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTacgac                                                                                                                      >  1:445700/1‑151 (MQ=255)                       cAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTacgacg                                                                                                                     <  1:177637/151‑1 (MQ=255)                        aGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGc                                                                                                                    >  2:707051/1‑151 (MQ=255)                         gTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGct                                                                                                                   <  1:540869/151‑1 (MQ=255)                             aTTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTAc                                                                                                               <  2:445751/151‑1 (MQ=255)                                cGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTAGACAGACAAATGAACTTATCCCTACGACGCTCTACCAg                                                                                                            <  1:138361/151‑1 (MQ=255)                                 gTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAgc                                                                                                           <  2:26771/151‑1 (MQ=255)                                 gTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAgc                                                                                                           >  1:201701/1‑151 (MQ=255)                                  ttAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAgcg                                                                                                          >  1:416497/1‑151 (MQ=255)                                           ggTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTg                                                                                                 >  1:226249/1‑151 (MQ=255)                                                cgacgGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCtcg                                                                                            >  1:679370/1‑151 (MQ=255)                                                     gCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCgtt                                                                                       <  1:307145/151‑1 (MQ=255)                                                       aTTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCgttgt                                                                                     >  2:543851/1‑151 (MQ=255)                                                           aTGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGttatt                                                                                 >  2:448064/1‑151 (MQ=255)                                                                 aaaTAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCAt                                                                           >  2:139458/1‑151 (MQ=255)                                                                 aaaTAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCAt                                                                           >  1:709301/1‑151 (MQ=255)                                                                  aaTAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATc                                                                          <  2:495763/151‑1 (MQ=255)                                                                         cGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGAcgcg                                                                   <  1:722770/151‑1 (MQ=255)                                                                          gCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACgcgg                                                                  >  1:516794/1‑151 (MQ=255)                                                                                  tCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGc                                                          >  1:569349/1‑151 (MQ=255)                                                                                      aTTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGcc                                                      <  2:201733/151‑1 (MQ=255)                                                                                         tttGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCAtt                                                   >  1:555336/1‑151 (MQ=255)                                                                                           tGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCAttt                                                  >  1:198250/1‑150 (MQ=255)                                                                                            gCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTAt                                                >  1:370955/1‑151 (MQ=255)                                                                                             ccATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATg                                               >  2:140142/1‑151 (MQ=255)                                                                                                       tCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACt                                     >  2:655492/1‑151 (MQ=255)                                                                                                          ttACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTg                                   >  2:741724/1‑150 (MQ=255)                                                                                                             cATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTc                               <  1:643303/151‑1 (MQ=255)                                                                                                               tctcTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGc                             >  1:692499/1‑151 (MQ=255)                                                                                                                   tAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGt                         >  2:173413/1‑151 (MQ=255)                                                                                                                    aaaacaaaacATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGt                         <  1:587277/150‑1 (MQ=255)                                                                                                                    aaaacaaaacATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGt                         >  1:127763/1‑150 (MQ=255)                                                                                                                          aaacaTAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCt                  >  1:64457/1‑151 (MQ=255)                                                                                                                            acaTAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGa                <  2:732144/151‑1 (MQ=255)                                                                                                                                aaCGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAgtg             <  1:586416/150‑1 (MQ=255)                                                                                                                                     aaCGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATc       <  2:621958/151‑1 (MQ=255)                                                                                                                                          aCTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATc  <  1:537825/151‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                  AGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCCGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATC  >  NC_000913/1621179‑1621466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑