Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,663,917	G→A	V103V (GTG→GTA)	ynfH →	oxidoreductase, membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,663,917	0	G	A	100.0%	145.6 / NA	39	V103V (GTG→GTA)	ynfH	oxidoreductase, membrane subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/17);  total (22/17)

GGGCGTCGGGTTTATTGCCTCTGTCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTATGGTGGCTGGTGGCAGTCATCGGTAAAATGCCGCAAGCGTTGGGGAAACTCTGGTTACTTTTCAGCATGGCGCTCGGCGTCATTTTCGTCTGGATGATGACCTGCGTGTATCAAATCGACACCGTGCCAACCTGGCATAACGGTTACACTACGCTGGCT  >  NC_000913/1663770‑1664064                                                                                                                                                    |                                                                                                                                                    gggCGTCGGGTTTATTGCCTCTGTCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTAtggtggctggtagc                                                                                                                                                   <  2:291416/150‑1 (MQ=255)     gTCGGGTTTATTGCCTCTGTCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTATGGTGGCTGGTAGCAGTCa                                                                                                                                              <  2:669769/151‑1 (MQ=255)         ggTTTATTGCCTCTGTCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTATGGTGGCTGGTAGCAGTCATCg                                                                                                                                           >  1:448876/1‑150 (MQ=255)            ttATTGCCTCTGTCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTATGGTGGCTGGTAGCAGTCATCGGTa                                                                                                                                        <  2:597451/150‑1 (MQ=255)                 gCCTCTGTCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTATGGTGGCTGGTAGCAGTCATCGGTAAAATGc                                                                                                                                  <  2:366087/151‑1 (MQ=255)                  ccTCTGTCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTATGGTGGCTGGTAGCAGTCATCGGTAAAATGcc                                                                                                                                 >  1:566648/1‑151 (MQ=255)                     ctGTCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTATGGTGGCTGGTAGCAGTCATCGGTAAAATGCCg                                                                                                                                <  1:285115/149‑1 (MQ=255)                     ctGTCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTATGGTGGCTGGTAGCAGTCATCGGTAAAATGCCGc                                                                                                                               >  1:172011/1‑150 (MQ=255)                       gTCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTATGGTGGCTGGTAGCAGTCATCGGTAAAATGCCGCa                                                                                                                              >  2:371782/1‑149 (MQ=255)                         cATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTATGGTGGCTGGTAGCAGTCATCGGTAAAATGCCGCAAGCg                                                                                                                          >  1:611471/1‑151 (MQ=255)                             cACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTATGGTGGCTGGTAGCAGTCATCGGTAAAATGCCGCAAGCGTTgg                                                                                                                      >  2:510677/1‑151 (MQ=255)                              aCCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTATGGTGGCTGGTAGCAGTCATCGGTAAAATGCCGCAAGCGTTggg                                                                                                                     <  1:115243/151‑1 (MQ=255)                              aCCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTATGGTGGCTGGTAGCAGTCATCGGTAAAATGCCGCAAGCGTTggg                                                                                                                     >  1:289069/1‑151 (MQ=255)                               ccTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTATGGTGGCTGGTAGCAGTCATCGGTAAAATGCCGCAAGCGTTgggg                                                                                                                    >  2:125583/1‑151 (MQ=255)                                cTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTATGGTGGCTGGTAGCAGTCATCGGTAAAATGCCGCAAGCGTTgggg                                                                                                                    >  2:540788/1‑150 (MQ=255)                                     aTCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTATGGTGGCTGGTAGCAGTCATCGGTAAAATGCCGCAAGCGTTGGGGAAAct                                                                                                               >  2:329189/1‑150 (MQ=255)                                        gCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTATGGTGGCTGGTAGCAGTCATCGGTAAAATGCCGCAAGCGTTGGGGAAACTCTgg                                                                                                           <  2:481859/151‑1 (MQ=255)                                         ccACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTATGGTGGCTGGTAGCAGTCATCGGTAAAATGCCGCAAGCGTTGGGGAAACTCTGGt                                                                                                          <  2:223536/151‑1 (MQ=255)                                              gCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGGAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTATGGCGGCTGGTAGCAGTCATCTGCAAAATGCCTCAAGCGTTGGGGAAACTCTGGTTACtt                                                                                                     >  1:202571/1‑151 (MQ=255)                                                 ggCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTATGGTGGCTGGTAGCAGTCATCGGTAAAATGCCGCAAGCGTTGGGGAAACTCTGGTTACTTTTc                                                                                                  >  2:405816/1‑151 (MQ=255)                                                  gCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTATGGTGGCTGGTAGCAGTCATCGGTAAAATGCCGCAAGCGTTGGGGAAACTCTGGTTACTTTTc                                                                                                  >  2:726223/1‑150 (MQ=255)                                                    gTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTATGGTGGCTGGTAGCAGTCATCGGTAAAATGCCGCAAGCGTTGGGGAAACTCTGGTTACTTTTCAGc                                                                                               <  1:168039/151‑1 (MQ=255)                                                     tttAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTATGGTGGCTGGTAGCAGTCATCGGTAAAATGCCGCAAGCGTTGGGGAAACTCTGGTTACTTTTCAGc                                                                                               >  1:559214/1‑150 (MQ=255)                                                     tttAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTATGGTGGCTGGTAGCAGTCATCGGTAAAATGCCGCAAGCGTTGGGGAAACTCTGGTTACTTTTCAGc                                                                                               >  1:198866/1‑150 (MQ=255)                                                       tAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTATGGTGGCTGGTAGCAGTCATCGGTAAAATGCCGCAAGCGTTGGGGAAACTCTGGTTACTTTTCAGCAt                                                                                             <  1:613650/150‑1 (MQ=255)                                                                 aatcgaatcgGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTATGGTGGCTGGTAGCAGTCATCGGTAAAATGCCGCAAGCGTTGGGGAAACTCTGGTTACTTTTCAGCATGGCGCTCGGCg                                                                                  >  2:423460/1‑151 (MQ=255)                                                                     gaatcgGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTATGGTGGCTGGTAGCAGTCATCGGTAAAATGCCGCAAGCGTTGGGGAAACTCTGGTTACTTTTCAGCATGGCGCTCGGCGTCAt                                                                              >  2:516488/1‑151 (MQ=255)                                                                         cgGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTATGGTGGCTGGTAGCAGTCATCGGTAAAATGCCGCAAGCGTTGGGGAAACTCTGGTTACTTTTCAGCATGGCGCTCGGCGTCAtttt                                                                           <  2:710053/150‑1 (MQ=255)                                                                         cgGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTATGGTGGCTGGTAGCAGTCATCGGTAAAATGCCGCAAGCGTTGGGGAAACTCTGGTTACTTTTCAGCATGGCGCTCGGCGTCATTTTc                                                                          <  2:269307/151‑1 (MQ=255)                                                                              cTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTATGGTGGCTGGTAGCAGTCATCGGTAAAATGCCGCAAGCGTTGGGGAAACTCTGGTTACTTTTCAGCATGGCGCTCGGCGTCATTTTCGTCTg                                                                     >  1:424043/1‑151 (MQ=255)                                                                                tCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTATGGTGGCTGGTAGCAGTCATCGGTAAAATGCCGCAAGCGTTGGGGAAACTCTGGTTACTTTTCAGCATGGCGCTCGGCGTCATTTTCGTCTgg                                                                    <  1:674896/150‑1 (MQ=255)                                                                                     tCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTATGGTGGCTGGTAGCAGTCATCGGTAAAATGCCGCAAGCGTTGGGGAAACTCTGGTTACTTTTCAGCATGGCGCTCGGCGTCATTTTCGTCTGGatgatg                                                              >  1:237158/1‑151 (MQ=255)                                                                                              cGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTATGGTGGCTGGTAGCAGTCATCGGTAAAATGCCGCAAGCGTTGGGGAAACTCTGGTTACTTTTCAGCATGGCGCTCGGCGTCATTTTCGTCTGGATGATGACCTGCgtg                                                     >  1:165461/1‑151 (MQ=255)                                                                                                                        ttGCCGTTGGCGGATTATGGTGGCTGGTAGCAGTCATCGGTAAAATGCCGCAAGCGTTGGGGAAACTCTGGTTACTTTTCAGCATGGCGCTCGGCGTCATTTTCGTCTGGATGATGACCTGCGTGTATCAAATCGACACCGTGCCAACCt                            <  1:318515/150‑1 (MQ=255)                                                                                                                         tGCCGTTGGCGGATTATGGTGGCTGGTAGCAGTCATCGGTAAAATGCCGCAAGCGTTGGGGAAACTCTGGTTACTTTTCAGCATGGCGCTCGGCGTCATTTTCGTCTGGATGATGACCTGCGTGTATCAAATCGACACCGTGCCAACCTg                           <  1:593852/150‑1 (MQ=255)                                                                                                                          gCCGTTGGCGGATTATGGTGGCTGGTAGCAGTCATCGGTAAAATGCCGCAAGCGTTGGGGAAACTCTGGTTACTTTTCAGCATGGCGCTCGGCGTCATTTTCGTCTGGATGATGACCTGCGTGTATCAAATCGACACCGTGCCAACCTgg                          <  1:726149/150‑1 (MQ=255)                                                                                                                          gCCGTTGGCGGATTATGGTGGCTGGTAGCAGTCATCGGTAAAATGCCGCAAGCGTTGGGGAAACTCTGGTTACTTTTCAGCATGGCGCTCGGCGTCATTTTCGTCTGGATGATGACCTGCGTGTATCAAATCGACACCGTGCCAACCTGGc                         >  1:123477/1‑151 (MQ=255)                                                                                                                            cGTTGGCGGATTATGGTGGCTGGTAGCAGTCATCGGTAAAATGCCGCAAGCGTTGGGGAAACTCTGGTTACTTTTCAGCATGGCGCTCGGCGTCATTTTCGTCTGGATGATGACCTGCGTGTATCAAATCGACACCGTGCCAACCTGGCAt                       >  1:611363/1‑151 (MQ=255)                                                                                                                             gTTGGCGGATTATGGTGGCTGGTAGCAGTCATCGGTAAAATGCCGCAAGCGTTGGGGAAACTCTGGTTACTTTTCAGCATGGCGCTCGGCGTCATTTTCGTCTGGATGATGACCTGCGTGTATCAAATCGACACCGTGCCAACCTGGCATa                      <  1:171688/151‑1 (MQ=255)                                                                                                                                   ggATTATGGTGGCTGGTAGCAGTCATCGGTAAAATGCCGCAAGCGTTGGGGAAACTCTGGTTACTTTTCAGCATGGCGCTCGGCGTCATTTTCGTCTGGATGATGACCTGCGTGTATCAAATCGACACCGTGCCAACCTGGCATAACGGtt                <  1:445434/151‑1 (MQ=255)                                                                                                                                             ggctggtagcAGTCATCGGTAAAATGCCGCAAGCGTTGGGGAAACTCTGGTTACTTTTCAGCATGGCGCTCGGCGTCATTTTCGTCTGGATGATGACCTGCGTGTATCAAATCGACACCGTGCCAACCTGGCATAACGGTTACACTACGCt      >  2:523069/1‑151 (MQ=255)                                                                                                                                               ctggtagcAGTCATCGGTAAAATGCCGCAAGCGTTGGGGAAACTCTGGTTACTTTTCAGCATGGCGCTCGGCGTCATTTTCGTCTGGATGATGACCTGCGTGTATCAAATCGACACCGTGCCAACCTGGCATAACGGTTACACTACGCTgg    >  2:452408/1‑151 (MQ=255)                                                                                                                                                 ggtagcAGTCATCGGTAAAATGCCGCAAGCGTTGGGGAAACTCTGGTTACTTTTCAGCATGGCGCTCGGCGTCATTTTCGTCTGGATGATGACCTGCGTGTATCAAATCGACACCGTGCCAACCTGGCATAACGGTTACACTACGCTgg    <  1:172576/149‑1 (MQ=255)                                                                                                                                                 ggtagcAGTCATCGGTAAAATGCCGCAAGCGTTGGGGAAACTCTGGTTACTTTTCAGCATGGCGCTCGGCGTCATTTTCGTCTGGATGATGACCTGCGTGTATCAAATCGACACCGTGCCAACCTGGCATAACGGTTACACTACGCTGGCt  <  2:406602/151‑1 (MQ=255)                                                                                                                                                 ggtagcAGTCATCGGTAAAATGCCGCAAGCGTTGGGGAAACTCTGGTTACTTTTCAGCATGGCGCTCGGCGTCATTTTCGTCTGGATGATGACCTGCGTGTATCAAATCGACACCGTGCCAACCTGGCATAACGGTTACACTACGCTGGCt  <  1:325072/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    GGGCGTCGGGTTTATTGCCTCTGTCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTATGGTGGCTGGTGGCAGTCATCGGTAAAATGCCGCAAGCGTTGGGGAAACTCTGGTTACTTTTCAGCATGGCGCTCGGCGTCATTTTCGTCTGGATGATGACCTGCGTGTATCAAATCGACACCGTGCCAACCTGGCATAACGGTTACACTACGCTGGCT  >  NC_000913/1663770‑1664064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑