Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,698,229	T→C	M318V (ATG→GTG)	malI ←	transcriptional repressor of Mal regulon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,698,229	0	T	C	100.0%	230.0 / NA	62	M318V (ATG→GTG)	malI	transcriptional repressor of Mal regulon
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/34);  total (28/34)

CCTGTGTGCCACATTTTTTATCATTAACCTTGAGGAAAGGACACTACCAGGTATAGCAGATAAAGAGAGGATTATTTCGCTGCAATGAGCCGGGCGGGAATAATAAGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCATGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGCTCAAAGTAACGATCGACTCCGCTTTCCCC  >  NC_000913/1698081‑1698373                                                                                                                                                     |                                                                                                                                                 ccTGTGTGCCACATTTTTTATCATTAACCTTGAGGAAAGGACACTACCAGGTATAGCAGATAAAGAGAGGATTATTTCGCTGCAATGAGCCGGGCGGGAATAATAAGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGc                                                                                                                                                <  1:347786/151‑1 (MQ=255)     tgtgCCACATTTTTTATCATTAACCTTGAGGAAAGGACACTACCAGGTATAGCAGATAAAGAGAGGATTATTTCGCTGCAATGAGCCGGGCGGGAATAATAAGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATc                                                                                                                                            >  2:220878/1‑151 (MQ=255)      gtgCCACATTTTTTATCATTAACCTTGAGGAAAGGACACTACCAGGTATAGCAGATAAAGAGAGGATTATTTCGCTGCAATGAGCCGGGCGGGAATAATAAGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATcc                                                                                                                                           >  1:378313/1‑151 (MQ=255)        gCCACATTTTTTATCATTAACCTTGAGGAAAGGACACTACCAGGTATAGCAGATAAAGAGAGGATTATTTCGCTGCAATGAGCCGGGCGGGAATAATAAGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCg                                                                                                                                          <  2:582819/150‑1 (MQ=255)            caTTTTTTATCATTAACCTTGAGGAAAGGACACTACCAGGTATAGCAGATAAAGAGAGGATTATTTCGCTGCAATGAGCCGGGCGGGAATAATAAGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAgt                                                                                                                                     <  2:452799/151‑1 (MQ=255)                       aTTAACCTTGAGGAAAGGACACTACCAGGTATAGCAGATAAAGAGAGGATTATTTCGCTGCAATGAGCCGGGCGGGAATAATAAGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCa                                                                                                                            >  2:644993/1‑149 (MQ=255)                          aaCCTTGAGGAAAGGACACTACCAGGTATAGCAGCTAAAGAGAGGATTATTTCGCTGCAATGAGCCGGGCGGGAATAATAAGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTc                                                                                                                       >  2:96092/1‑151 (MQ=255)                            ccTTGAGGAAAGGACACTACCAGGTATAGCAGATAAAGAGAGGATTATTTCGCTGCAATGAGCCGGGCGGGAATAATAAGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTccc                                                                                                                     <  2:550083/151‑1 (MQ=255)                            ccTTGAGGAAAGGACACTACCAGGTATAGCAGATAAAGAGAGGATTATTTCGCTGCAATGAGCCGGGCGGGAATAATAAGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTccc                                                                                                                     >  2:254432/1‑151 (MQ=255)                                gatgAAAGGACACTACCAGGTATAGCAGATAAAGAGAGGATTATTTCGCTGCAATGAGCCGGGCGGGAATAATAAGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCcgcg                                                                                                                  <  2:316843/147‑1 (MQ=255)                                      aGGACACTACCAGGTATAGCAGATAAAGAGAGGATTATTTCGCTGCAATGAGCCGGGCGGGAATAATAAGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGt                                                                                                           <  2:171307/151‑1 (MQ=255)                                      aGGACACTACCAGGTATAGCAGATAAAGAGAGGATTATTTCGCTGCAATGAGCCGGGCGGGAATAATAAGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGt                                                                                                           <  1:733185/151‑1 (MQ=255)                                            cTACCAGGTATAGCAGATAAAGAGAGGATTATTTCGCTGCAATGAGCCGGGCGGGAATAATAAGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGc                                                                                                     >  1:126261/1‑151 (MQ=255)                                             tACCAGGTATAGCAGCTAAAGAGAGGATTATTTCGCTGCAATGAGCCGGGCGGGAATAATAAGCTTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGcc                                                                                                    >  1:632849/1‑151 (MQ=255)                                               ccAGGTATAGCAGATAAAGAGAGGATTATTTCGCTGCAATGAGCCGGGCGGGAATAATAAGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCa                                                                                                  <  2:579064/151‑1 (MQ=255)                                               ccAGGTATAGCAGATAAAGAGAGGATTATTTCGCTGCAATGAGCCGGGCGGGAATAATAAGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCa                                                                                                  <  2:126293/151‑1 (MQ=255)                                                 aGGTATAGCAGATAAAGAGAGGATTATTTCGCTGCAATGAGCCGGGCGGGAATAATAAGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAgg                                                                                                <  1:432431/151‑1 (MQ=255)                                                      taGCAGATAAAGAGAGGATTATTTCGCTGCAATGAGCCGGGCGGGAATAATAAGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAAc                                                                                            >  1:684152/1‑150 (MQ=255)                                                       aGCAGATAAAGAGAGGATTATTTCGCTGCAATGAGCCGGGCGGGAATAATAAGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAAc                                                                                            >  2:456243/1‑149 (MQ=255)                                                       aGCAGATAAAGAGAGGATTATTTCGCTGCAATGAGCCGGGCGGGAATAATAAGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAg                                                                                          <  1:405013/151‑1 (MQ=255)                                                         cAGATAAAGAGAGGATTATTTCGCTGCAATGAGCCGGGCGGGAATAATAAGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGt                                                                                        <  1:103994/151‑1 (MQ=255)                                                         cAGATAAAGAGAGGATTATTTCGCTGCAATGAGCCGGGCGGGAATAATAAGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGt                                                                                        <  1:544307/151‑1 (MQ=255)                                                         cAGATAAAGAGAGGATTATTTCGCTGCAATGAGCCGGGCGGGAATAATAAGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGt                                                                                        >  2:305697/1‑151 (MQ=255)                                                          aGATAAAGAGAGGATTATTTCGCTGCAATGAGCCGGGCGGGAATAATAAGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGt                                                                                        >  2:194847/1‑150 (MQ=255)                                                          aGATAAAGAGAGGATTATTTCGCTGCAATGAGCCGGGCGGGAATAATAAGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGt                                                                                        >  2:173591/1‑150 (MQ=255)                                                            aTAAAGAGAGGATTATTTCGCTGCAATGAGCCGGGCGGGAATAATAAGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGtat                                                                                      >  2:158660/1‑150 (MQ=255)                                                                agagagGATTATTTCGCTGCAATGAGCCGGGCGGGAATAATAAGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGTATatcat                                                                                 >  2:181952/1‑151 (MQ=255)                                                                agagagGATTATTTCGCTGCAATGAGCCGGGCGGGAATAATAAGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGTATatcat                                                                                 >  1:549962/1‑151 (MQ=255)                                                                  agagGATTATTTCGCTGCAATGAGCCGGGCGGGAATAATAAGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGTATatcatc                                                                                >  1:214414/1‑150 (MQ=255)                                                                        ttattTCGCTGCAATGAGCCGGGCGGGAATAATAAGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGTATATCATCAAGtgtg                                                                         >  1:346620/1‑151 (MQ=255)                                                                         tattTCGCTGCAATGAGCCGGGCGGGAATAATAAGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGTATATCATCAAGtgtg                                                                         <  2:723636/150‑1 (MQ=255)                                                                          attTCGCTGCAATGAGCCGGGCGGGAATAATAAGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGTATATCATCAAGTGtggt                                                                       <  2:773458/151‑1 (MQ=255)                                                                            ttCGCTGCAATGAGCCGGGCGGGAATAATAAGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGTATATCATCAAGTGtggtt                                                                      <  1:585816/150‑1 (MQ=255)                                                                            ttCGCTGCAATGAGCCGGGCGGGAATAATAAGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGTATATCATCAAGTGtggtt                                                                      <  2:197429/150‑1 (MQ=255)                                                                              cGCTGCAATGAGCCGGGCGGGAATAATAAGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGTATATCATCAAGTGtggttggt                                                                   <  2:684226/151‑1 (MQ=255)                                                                              cGCTGCAATGAGCCGGGCGGGAATAATAAGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGTATATCATCAAGTGtggttggt                                                                   >  1:340151/1‑151 (MQ=255)                                                                                cTGCAATGAGCCGGGCGGGAATAATAAGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGTATATCATCAAGTGTGGTTGgtgt                                                                 >  1:772029/1‑151 (MQ=255)                                                                                  gCAATGAGCCGGGCGGGAATAATAAGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGTATATCATCAAGTGTGGTTGgtgt                                                                 <  1:391371/149‑1 (MQ=255)                                                                                  gCAATGAGCCGGGCGGGAATAATAAGATTGCATGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGTATATCATCAAGTGTGGTTGGTGTc                                                                <  2:600582/150‑1 (MQ=255)                                                                                           cgggcgggAATAATAAGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTa                                                       >  1:345763/1‑150 (MQ=255)                                                                                                gggAATAATAAGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATgc                                                  <  2:74148/150‑1 (MQ=255)                                                                                                     taataaGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATgccgcc                                              >  1:610091/1‑149 (MQ=255)                                                                                                       ataaGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCg                                          >  1:708526/1‑151 (MQ=255)                                                                                                              tGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTg                                   >  1:579994/1‑151 (MQ=255)                                                                                                               gCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGt                                  <  1:390246/151‑1 (MQ=255)                                                                                                                cGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGtt                                 >  2:72086/1‑151 (MQ=255)                                                                                                                 gTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTg                                <  1:344370/151‑1 (MQ=255)                                                                                                                 gTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTg                                <  2:228911/151‑1 (MQ=255)                                                                                                                   gAATGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGCt                              >  2:279323/1‑151 (MQ=255)                                                                                                                     aTGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGCTCa                            <  1:150466/151‑1 (MQ=255)                                                                                                                     aTGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGCTCa                            <  2:755322/151‑1 (MQ=255)                                                                                                                      tGCGTCTCTTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGCTCa                            >  1:593032/1‑150 (MQ=255)                                                                                                                             ctTCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGCTCAAAGTa                       <  2:135378/148‑1 (MQ=255)                                                                                                                               tCATGGGTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGCTCAAAGTAACg                    <  2:223689/149‑1 (MQ=255)                                                                                                                                   gggTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGCTCAAAGTAACGATCGa               <  1:140091/150‑1 (MQ=255)                                                                                                                                   gggTGATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGCTCAAAGTAACGATCGAc              >  1:695125/1‑151 (MQ=255)                                                                                                                                       gATTTTTTGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGCTCAAAGTAACGATCGACTCCg          >  1:334932/1‑151 (MQ=255)                                                                                                                                           ttttGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGCTCAAAGTAACGATCGACTCCGCtt       <  1:344635/150‑1 (MQ=255)                                                                                                                                           ttttGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGCTCAAAGTAACGATCGACTCCGCtt       <  2:155126/150‑1 (MQ=255)                                                                                                                                            tttGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGCTCAAAGTAACGATCGACTCCGCttt      <  1:456192/150‑1 (MQ=255)                                                                                                                                              tGCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGCTCAAAGTAACGATCGACTCCGCTTTcc    <  1:35757/150‑1 (MQ=255)                                                                                                                                               gCATCACGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGCTCAAAGTAACGATCGACTCCGCTTTcccc  <  2:256515/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                 CCTGTGTGCCACATTTTTTATCATTAACCTTGAGGAAAGGACACTACCAGGTATAGCAGATAAAGAGAGGATTATTTCGCTGCAATGAGCCGGGCGGGAATAATAAGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCATGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGCTCAAAGTAACGATCGACTCCGCTTTCCCC  >  NC_000913/1698081‑1698373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑