Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,994,461 (T)8→7 pseudogene (632/633 nt) ygeK ← pseudogene, response regulator family, part of T3SS PAI ETT2 remnant; putative 2‑component transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,994,4540T.100.0% 288.5 / NA 60intergenic (+13/+6)pbl/ygeKpseudogene, peptidoglycan‑binding enzyme family/pseudogene, response regulator family, part of T3SS PAI ETT2 remnant; putative 2‑component transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (23/37);  total (23/37)

ACGTGGCAATGCCTGGGGACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCAAGAAATTAGCGATTTCT  >  NC_000913/2994305‑2994595
                                                                                                                                                     |                                                                                                                                             
aCGTGGCAATGCCTGGGGACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCtt                                                                                                                                              <  2:309219/151‑1 (MQ=255)
  gTGGCAATGCCTGGGGACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCtttt                                                                                                                                            <  1:290764/151‑1 (MQ=255)
    ggCAATGCCTGGGGACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTATAGCCTCtttttt                                                                                                                                          <  1:281515/151‑1 (MQ=255)
              ggggACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTg                                                                                                                                <  1:362543/150‑1 (MQ=255)
               tggACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCCGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGt                                                                                                                                 <  2:470888/147‑1 (MQ=255)
               gggACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCa                                                                                                                              <  2:524939/151‑1 (MQ=255)
                      ataATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACAccc                                                                                                                       >  1:504896/1‑151 (MQ=255)
                        aaTGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCAt                                                                                                                     <  2:343847/151‑1 (MQ=255)
                        aaTGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCAt                                                                                                                     <  1:644321/151‑1 (MQ=255)
                          tGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATAc                                                                                                                   >  2:350831/1‑151 (MQ=255)
                              ggTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTaa                                                                                                               <  1:23121/151‑1 (MQ=255)
                              ggTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTa                                                                                                                >  1:399678/1‑150 (MQ=255)
                                 tttGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAg                                                                                                             <  1:226223/150‑1 (MQ=255)
                                     cAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGtt                                                                                                        <  2:616279/151‑1 (MQ=255)
                                       aTGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGtttt                                                                                                      <  1:84628/151‑1 (MQ=255)
                                         ggATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTg                                                                                                     >  2:373019/1‑150 (MQ=255)
                                         ggATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGa                                                                                                    <  2:448723/151‑1 (MQ=255)
                                           aTAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATc                                                                                                  <  1:327075/151‑1 (MQ=255)
                                             aaCCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCa                                                                                                 <  1:46014/150‑1 (MQ=255)
                                               ccAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACt                                                                                              <  2:628827/151‑1 (MQ=255)
                                                cAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTc                                                                                             <  2:553221/151‑1 (MQ=255)
                                                   aaaaaGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGAt                                                                                          >  2:638816/1‑151 (MQ=255)
                                                    aaaaGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATa                                                                                         <  2:608250/151‑1 (MQ=255)
                                                     aaaGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATAc                                                                                        >  2:479989/1‑151 (MQ=255)
                                                       agagACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATAcc                                                                                       >  1:787680/1‑150 (MQ=255)
                                                                aaTATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAAc                                                                              >  2:514702/1‑150 (MQ=255)
                                                                   atGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAAc                                                                              <  1:59961/147‑1 (MQ=255)
                                                                   atGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCt                                                                          <  2:707857/151‑1 (MQ=255)
                                                                   atGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCt                                                                          <  2:728272/151‑1 (MQ=255)
                                                                     gCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTa                                                                         <  1:350857/150‑1 (MQ=255)
                                                                      ccccAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAAt                                                                       <  2:130643/151‑1 (MQ=255)
                                                                          aaaaTATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATttct                                                                   <  1:373315/151‑1 (MQ=255)
                                                                          aaaaTATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATttct                                                                   >  2:102850/1‑151 (MQ=255)
                                                                             atatatatTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCa                                                                <  1:436909/151‑1 (MQ=255)
                                                                              tatatatTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCACAATGAGACTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCAt                                                               >  2:370076/1‑151 (MQ=255)
                                                                              tatatatTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCAt                                                               >  2:236228/1‑151 (MQ=255)
                                                                                   atTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGatat                                                          <  2:40693/151‑1 (MQ=255)
                                                                                          ataCCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCt                                                   <  1:135508/151‑1 (MQ=255)
                                                                                           taCCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCt                                                   >  1:324796/1‑150 (MQ=255)
                                                                                                    tAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCg                                         >  1:172461/1‑151 (MQ=255)
                                                                                                       tGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACt                                      >  1:889644/1‑151 (MQ=255)
                                                                                                          aCTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGtt                                   <  1:543634/151‑1 (MQ=255)
                                                                                                             aGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGtttt                                 <  2:207/150‑1 (MQ=255)
                                                                                                             aGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGttt                                  <  2:264050/149‑1 (MQ=255)
                                                                                                             aGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTc                                <  1:514752/151‑1 (MQ=255)
                                                                                                             aGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTc                                <  2:504847/151‑1 (MQ=255)
                                                                                                                 aaTCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATTATATTGAGTCTCTGAGTTTCGACTGTTTTCCTGc                            >  2:195541/1‑151 (MQ=255)
                                                                                                                    cGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCtt                          >  1:873859/1‑150 (MQ=255)
                                                                                                                      tAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAAc                       >  1:862431/1‑151 (MQ=255)
                                                                                                                       aaGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACt                      <  1:328683/151‑1 (MQ=255)
                                                                                                                             tCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACATCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCAAg                 <  1:760729/150‑1 (MQ=255)
                                                                                                                              cAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCAAGaa               <  1:301870/151‑1 (MQ=255)
                                                                                                                              cAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCAAGaa               <  2:420949/151‑1 (MQ=255)
                                                                                                                                  aaaaTGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCGTGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCAAGAAAtt            >  2:190382/1‑150 (MQ=255)
                                                                                                                                  aaaaTGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCAAGAAAtt            >  1:888913/1‑150 (MQ=255)
                                                                                                                                   aaaTGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCAAGAAAtt            >  2:626876/1‑149 (MQ=255)
                                                                                                                                   aaaTGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCAAGAAATTAg          <  2:717075/151‑1 (MQ=255)
                                                                                                                                     aTGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCAAGAAATTAGCg        >  2:838356/1‑151 (MQ=255)
                                                                                                                                      tGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCAAGAAAtt            <  2:615342/146‑1 (MQ=255)
                                                                                                                                      tGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCAAGAAAtt            >  1:615391/1‑146 (MQ=255)
                                                                                                                                         gCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCAAGAAATTAGCGAttt    <  1:339760/151‑1 (MQ=255)
                                                                                                                                           cTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCAAGAAATTAGCGATTTCt  >  1:364637/1‑151 (MQ=255)
                                                                                                                                             aaaGCCTCTTTTTTTATATAGTGCAAACTCCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCAAGAAATTAGCGATTTCt  <  2:581835/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                             
ACGTGGCAATGCCTGGGGACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCAAGAAATTAGCGATTTCT  >  NC_000913/2994305‑2994595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: