| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 483,728 | Δ1 bp | coding (676/3150 nt) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 483,726 | 0 | T | . | 92.1% | 142.2 / 6.4 | 41 | coding (678/3150 nt) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base T (0/2); major base . (26/9); minor base C (0/3); total (26/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.61e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCG > NC_000913/483578‑483870 | cGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCttc > 1:760098/1‑150 (MQ=255)cGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCtt < 2:329152/150‑1 (MQ=255) tAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCttcac > 1:103066/1‑148 (MQ=255) gTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCC‑TTCACcg < 1:613661/151‑1 (MQ=255) tctcACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCC‑TTCACcggc > 2:81428/1‑151 (MQ=255) caccacCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGG‑CATTCACcggc < 2:902071/148‑1 (MQ=255) ccaccCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCC‑TTCACCGgcggcg < 2:360417/150‑1 (MQ=255) cccAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCC‑TTCACCGGCGGCGTac < 2:179005/150‑1 (MQ=255) aGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCC‑TTCACCGGCGGCGTaccacc < 2:852888/151‑1 (MQ=255) gACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCTGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCC‑TTCACCGGCGGCGTACCACCGAGCTGACccgccg > 1:802308/1‑151 (MQ=255) cACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCC‑TTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAAcc > 1:56461/1‑151 (MQ=255) aCGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCC‑TTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCt > 2:305970/1‑151 (MQ=255) gggggAACCTTCCTTATTCCCTTTCAGGAGGATTTGCCCAACCTCTTCAGTAGAGGTCGGACGCGTCTAGGAAAAAAGAGGGGCTTTAAGTTGTTGGCC‑TTCCCCGGGGGCGTCCCCCCGGGCTGCCCCGCCGCAACCTGGGCGTTCTg < 1:396356/147‑1 (MQ=255) cgGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGCCAGACGCGTCTGAGCAATAATAGAGGCTTTAACCTGTTGGCC‑TTCACCGGCGGCGTACCACGGAGCTGACCCGCCGCAACCTGGGCGt > 1:277893/1‑144 (MQ=255) ccATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCC‑TTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGa > 1:621000/1‑150 (MQ=255) tCCTGTTTCTCTCCCAGCAGATTTTTGCCGAACTCTTGAGGAGAGGTCAGCCGCTTCTGAGAAATAATAGGTGCTTGAGGCTGTTGG‑CTTCCTCCGGCGTCGACCCCCAGAGCTACCCCGCCGCAACCTGGGCGTTCTGCGCtt < 2:187147/144‑1 (MQ=255) tCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCC‑TTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGc > 2:853257/1‑151 (MQ=255) cTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCC‑TTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCgg > 2:468422/1‑151 (MQ=255) gATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCC‑TTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCgg > 2:619805/1‑149 (MQ=255) ttCTCTTTCAGGAGTTTTTTGCCGAACTCTTCAGTAGAGGTCAGCCGCGTCTGAGAAATAAAAGGGGCTTTAAGTTGTGGGCC‑TCCCCCGGCGGCGTACCCCCGGGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTa < 1:866750/149‑1 (MQ=255) ttCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCC‑TTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATc > 1:447618/1‑151 (MQ=255) cagcagGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATATTAGAGGCGTTAAGCTGTTGGCC‑TTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTCATGAc > 2:597998/1‑146 (MQ=255) cagGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCC‑TTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAAcc > 1:424616/1‑150 (MQ=255) ggtttttCCCAAACTCTTGAGTAGGGGCCAGACGCGTCTGAGAAATAATGGGGGC‑TTAAGGTTGTTGCCTTCCCCGGGGGGCGTCCCACCGAGCTGCCCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAAc < 1:720174/144‑1 (MQ=255) aTTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCC‑TTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGc > 1:159327/1‑149 (MQ=255) ttttGCCGAACTCTTCAGTGGGGGTCAGCCGCGTCTGAGAAAAAATAGGGGCGTTAAGCTGTT‑GCCCTCCCCCGGCGGCTTACCCCCGGTCTGCCCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACAt < 2:385787/140‑1 (MQ=255) tttGCCGAACTCTTAAGTAGAGGTCAGCCGCGTCTGAGAAAAAATAGGGGGTTTAGGTTGTGGGCC‑TTCCCCGGCGGCGTCCCCCCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGtt < 1:841938/150‑1 (MQ=255) ttggCCGAACTCTTCAGGAGAGGTCGCCCGCGCCTGAGAAAAAATGGGGCGGGTTGTCTGTGCGCC‑TCCCCCGGGGGCGTACCACCGAGCCGCCCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGtt < 2:465468/147‑1 (MQ=255) ccGAACTCTGCAGTAGGGGTCAGACGCGTCGGAGAAAAAATAGGGGCGTTAGGTTGTT‑GCCCTTCCCCGGGGGCGTCCCCCCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTa < 1:241289/147‑1 (MQ=255) aaCTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCC‑TTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGa > 1:905143/1‑150 (MQ=255) aaCTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCC‑TTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGa > 2:533583/1‑150 (MQ=255) ttCAGTAGGGGTCAGACGCGTCTGAGAAATAAGAGAGGGTTTAGGCTGTTGGCC‑TCCCCCGGCGGCGTCCCCCCGAGCTGCCCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTgg < 1:768351/144‑1 (MQ=255) gTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCC‑TTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCAtt > 2:526420/1‑151 (MQ=255) cAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCC‑TTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCg > 2:134885/1‑151 (MQ=255) aCGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCC‑TTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGt > 1:638354/1‑151 (MQ=255) cgcgTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCC‑TTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGtt > 1:728443/1‑151 (MQ=255) cgcgTCTGAAAAATAATAGAGGCGTTATG‑TTTTTGCCCTCCCCCGGCGGCGTCCCCCCAAGCTGCCCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCggg < 2:875122/149‑1 (MQ=255) aGCAATAATAGAGGCGTTAAGCTGTTGGCC‑TTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATcc > 1:92145/1‑148 (MQ=255) aataatAGAGGCGTTAAGCTGTTGGCC‑TTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTc > 1:599166/1‑131 (MQ=255) taataGAGGCGTTAAGCTGTTGGCC‑TTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCCGCTCATTCGGGTTCATCCAGATACGc > 1:314262/1‑151 (MQ=255) taataGAGGCGTTAAGCTGTTGGCC‑TTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGc > 2:62547/1‑151 (MQ=255) gagGCGTTAAGCTGTTGGCC‑TTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATcgc > 1:133623/1‑151 (MQ=255) cGTTAAGCTGTTGGCC‑TTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTAc > 2:206164/1‑151 (MQ=255) ttGGCC‑TTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCg > 1:817631/1‑150 (MQ=255) | CGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCG > NC_000913/483578‑483870 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |