Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,812,657	(T)7→8	coding (476/804 nt)	ydjO ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,812,650	1	.	T	98.7%	291.4 / NA	76	N161K (AAT→AAA)	ydjO	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base . (0/1);  major base T (40/34);  minor base G (0/1);  total (40/36)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCATACCCGAGACGTTTTCTAAGTGAACTATCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCA‑TTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATACTGATCAAATTGGTCATTTTTTCTGGGCGAAATAC  >  NC_000913/1812501‑1812784                                                                                                                                                       |                                                                                                                                        tCATACCCGAGACGTTTTCTAAGTGAACTATCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCA‑t                                                                                                                                        >  1:469700/1‑151 (MQ=255)     aCCCGAGACGTTTTCTAAGTGAACTATCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTGTCGAAGGAAACTTTAGGAGACTCTCA‑ttttt                                                                                                                                    >  2:785038/1‑151 (MQ=255)       ccGAGACGTTTTCTAAGTGAACTATCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCA‑ttttttt                                                                                                                                  >  2:388439/1‑151 (MQ=255)       ccGAGACGTTTTCTAAGTGAACTATCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCA‑ttttttt                                                                                                                                  >  2:709605/1‑151 (MQ=255)         gagaCGTTTTCTAAGTGAACTATCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCA‑ttttttttg                                                                                                                                >  2:595770/1‑149 (MQ=255)                tttCTAAGTGAACTATCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCgc                                                                                                                          >  1:555769/1‑150 (MQ=255)                tttCTAAGTGAACTATCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCgc                                                                                                                          >  2:121447/1‑150 (MQ=255)                     aaGTGAACTATCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCaaa                                                                                                                    <  1:790855/151‑1 (MQ=255)                     aaGTGAACTATCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCaa                                                                                                                     <  1:477927/150‑1 (MQ=255)                         gAACTATCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATg                                                                                                                >  1:884310/1‑151 (MQ=255)                            cTATCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTc                                                                                                             >  2:434287/1‑151 (MQ=255)                               tCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCaaa                                                                                                                    <  2:884800/141‑1 (MQ=255)                               tCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCaaa                                                                                                                    >  1:884871/1‑141 (MQ=255)                                cTGGAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCg                                                                                                          >  2:224979/1‑150 (MQ=255)                                cTGGAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCg                                                                                                          >  1:239934/1‑150 (MQ=255)                                  ggAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTccc                                                                                                           <  2:718368/147‑1 (MQ=255)                                           ataataTCTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGtat                                                                                               >  2:380063/1‑150 (MQ=255)                                                atCTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCAGTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAAt                                                                                          <  2:83771/150‑1 (MQ=255)                                                  cTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGCTATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAAtttt                                                                                       >  2:750287/1‑151 (MQ=255)                                                    tCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAAtt                                                                                         <  2:751573/147‑1 (MQ=255)                                                     cAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGtt                                                                                    <  2:597622/151‑1 (MQ=255)                                                     cAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGtt                                                                                    <  1:832272/151‑1 (MQ=255)                                                     cAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCA‑TTTTTTTCGAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGt                                                                                     <  1:434334/150‑1 (MQ=255)                                                      aaGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTc                                                                                   >  1:860969/1‑151 (MQ=255)                                                        gCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTGTCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCAt                                                                                 <  1:785089/151‑1 (MQ=255)                                                        gCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCAt                                                                                 <  1:541531/151‑1 (MQ=255)                                                          acacCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAg                                                                               <  1:198939/151‑1 (MQ=255)                                                           caccacTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGAAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGt                                                                              <  1:705664/151‑1 (MQ=255)                                                             ccacTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGta                                                                             <  2:91783/150‑1 (MQ=255)                                                               acTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGtatat                                                                          >  2:352690/1‑151 (MQ=255)                                                               acTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGtatat                                                                          >  1:841575/1‑151 (MQ=255)                                                                 ttCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGtatat                                                                          >  1:425515/1‑149 (MQ=255)                                                                 ttCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGtatat                                                                          >  1:276708/1‑149 (MQ=255)                                                                  tCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGt                                                                       >  1:431683/1‑151 (MQ=255)                                                                   cccATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTc                                                                      >  2:272135/1‑151 (MQ=255)                                                                      aTTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCtt                                                                   <  2:211370/151‑1 (MQ=255)                                                                      aTTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCtt                                                                   <  1:234856/151‑1 (MQ=255)                                                                      aTTACCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCtt                                                                   <  2:705864/151‑1 (MQ=255)                                                                         cccGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTaaa                                                                <  2:358431/151‑1 (MQ=255)                                                                         cccGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTaa                                                                 <  2:577154/150‑1 (MQ=255)                                                                         cccGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTaa                                                                 <  1:274746/150‑1 (MQ=255)                                                                         cccGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTaa                                                                 >  1:188535/1‑150 (MQ=255)                                                                          ccGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAAt                                                               <  1:595819/151‑1 (MQ=255)                                                                           cGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAAt                                                               >  1:563662/1‑150 (MQ=255)                                                                             gAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAg                                                             >  2:874062/1‑150 (MQ=255)                                                                             gAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGt                                                            <  2:672903/151‑1 (MQ=255)                                                                                   ttAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATa                                                       >  1:53459/1‑150 (MQ=255)                                                                                   ttAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATa                                                       <  2:421572/150‑1 (MQ=255)                                                                                   ttAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATa                                                       <  2:25158/150‑1 (MQ=255)                                                                                     aGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATaacc                                                    >  2:430600/1‑151 (MQ=255)                                                                                     aGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATaacc                                                    >  2:510628/1‑151 (MQ=255)                                                                                        ggATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATaaccaac                                                 <  2:242217/151‑1 (MQ=255)                                                                                             aaaaaGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGtt                                            >  2:880137/1‑151 (MQ=255)                                                                                               aaaGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGtttt                                          <  1:66636/151‑1 (MQ=255)                                                                                               aaaGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGtttt                                          <  1:157662/151‑1 (MQ=255)                                                                                                  gaagaTTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTta                                       <  2:511115/151‑1 (MQ=255)                                                                                                    agaTTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTtata                                     <  1:277062/151‑1 (MQ=255)                                                                                                    agaTTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTtat                                      >  2:786903/1‑150 (MQ=255)                                                                                                     gaTTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATAc                                    >  1:632701/1‑151 (MQ=255)                                                                                                       ttGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAATCTGTTTTTTATACt                                   >  1:404041/1‑150 (MQ=255)                                                                                                            atTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATACTGAtta                              <  1:121469/150‑3 (MQ=255)                                                                                                            atTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATACTGATCaa                             <  2:735839/151‑1 (MQ=255)                                                                                                              tAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATACTGATCAAAt                           >  1:532992/1‑151 (MQ=255)                                                                                                               aaGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATACTGATCAAAtt                          >  1:804535/1‑151 (MQ=255)                                                                                                               aaGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATACTGATCAAAt                           <  1:496307/150‑1 (MQ=255)                                                                                                                aGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATACTGATCAAATTg                         <  2:884239/151‑1 (MQ=255)                                                                                                                 gCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATACTGATCAAATTgg                        >  2:372777/1‑151 (MQ=255)                                                                                                                      gATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATACTGATCAAATTGGTCAtt                   >  1:737506/1‑151 (MQ=255)                                                                                                                      gATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATACTGATCAAATTGGTCAtt                   >  2:346842/1‑151 (MQ=255)                                                                                                                      gATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATACTGATCAAATTGGTCAtt                   >  1:880341/1‑151 (MQ=255)                                                                                                                       atatTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATACTGATCAAATTGGTCAttt                  <  1:680922/151‑1 (MQ=255)                                                                                                                         attatCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATACTGATCAAATTGGTCAttttt                >  2:878795/1‑151 (MQ=255)                                                                                                                             tCGAAGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATACTGATCAAATTGGTCATTTTTTCTg            >  1:755823/1‑151 (MQ=255)                                                                                                                                aaGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATACTGATCAAATTGGTCATTTTTTCTGGGc         <  1:21343/151‑1 (MQ=255)                                                                                                                                 aGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATACTGATCAAATTGGTCATTTTTTCTggg          <  1:683331/149‑1 (MQ=255)                                                                                                                                 aGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATACTGATCAAATTGGTCATTTTTTCTGGGCg        >  2:469745/1‑151 (MQ=255)                                                                                                                                 aGGAAACTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATACTGATCAAATTGGTCATTTTTTCTGGGCg        >  2:748749/1‑151 (MQ=255)                                                                                                                                     aaCTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATACTGATCAAATTGGTCATTTTTTCTGGGCGAAAt    >  2:135756/1‑151 (MQ=255)                                                                                                                                       cTTTAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATACTGATCAAATTGGTCATTTTTTCTGGGCGAAATAc  >  1:550755/1‑151 (MQ=255)                                                                                                                                          tAGGCGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATACTGATCAAATTGGTCATTTTTTCTGGGCGAAATAc  >  1:699742/1‑148 (MQ=255)                                                                                                                                          tAGGAGACTCTCATTTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATACTGATCAAATTGGTCATTTTTTCTGGGCGAAATAc  >  2:5943/1‑148 (MQ=255)                                                                                                                                                       |                                                                                                                                        TCATACCCGAGACGTTTTCTAAGTGAACTATCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCA‑TTTTTTT‑GAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATACTGATCAAATTGGTCATTTTTTCTGGGCGAAATAC  >  NC_000913/1812501‑1812784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑