| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,967,611 | (C)5→6 | coding (891/1050 nt) | cheB ← | fused chemotaxis regulator: protein‑glutamate methylesterase in two‑component regulatory system with CheA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,967,606 | 1 | . | C | 92.3% | 123.4 / 4.0 | 40 | coding (896/1050 nt) | cheB | fused chemotaxis regulator: protein‑glutamate methylesterase in two‑component regulatory system with CheA |
| Reads supporting (aligned to +/- strand): ref base . (0/3); new base C (19/17); total (20/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
ACGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCAT‑G‑CCCC‑CGCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCGAAGGCCGATGACGGT > NC_000913/1967458‑1967741 | aCGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGTGTCCAT‑G‑c < 1:908385/150‑1 (MQ=255)aCGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCAT‑G‑cc > 2:259531/1‑151 (MQ=255)aCGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCAT‑G‑cc > 1:870162/1‑151 (MQ=255) aTCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCGGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCAT‑G‑cccc‑cc < 1:902585/151‑2 (MQ=255) tCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCAT‑G‑cccc‑cc > 1:429623/1‑149 (MQ=255) ccTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCAT‑G‑cccc‑ccgcct > 1:607887/1‑146 (MQ=255) gTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCAT‑GCCCCC‑CGCCTGa < 1:239495/150‑1 (MQ=255) gTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCAT‑GCCCCC‑CGCCTGAc > 1:478225/1‑151 (MQ=255) cGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCAT‑GCCCCC‑CGCCTGACGc > 1:450662/1‑150 (MQ=255) gCACTAATCTTTGCCAACATTTGCTGGCTTACCTGGCTAAGTTCGATCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCCAACCCCACGCACCCTTCTTCGCTTTGCGCACGGGTCCAT‑GCCCCC‑CGCCTGCCGCa > 2:387518/1‑149 (MQ=255) aCTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCAT‑GCCCCC‑CGCCTGACGCATCGc > 1:106855/1‑151 (MQ=255) aaTTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCAT‑GCCCCC‑CGCCTGACGCATCGcc > 1:510603/1‑149 (MQ=255) gCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCAT‑GCCCCC‑CGCCTGACGCATCGCCAACATTccc < 1:394245/151‑1 (MQ=255) gCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCAT‑GCCCCC‑CGCCTGACGCATCGCCAACATTcc < 1:56127/150‑1 (MQ=255) cAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCAT‑GCCCCC‑CGCCTGACGCATCGCCAACATTCccgc < 1:544650/151‑1 (MQ=255) gCTAACCTGTCTAGGATCGAACACTCCGAAGAGCACACCCATATTATAGCCCTCGCCCGGCAGGCCGAACACCACGCAGTGGGCTGGGTTTGGCGCAAGGGCCCAA‑GCCCCC‑CGACGGGCGGAACGCCAACATTCCCGCCGCGCcgtc < 2:449892/148‑1 (MQ=255) tAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCAT‑GCCCCC‑CGCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCgg > 1:607183/1‑151 (MQ=255) tAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCAT‑GCCCCC‑CGCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCgg > 1:189004/1‑151 (MQ=255) tAAGATCGACCACTCCGCAGACCCCCCCCATATTGATGGCCTCGCGCGCCATGCCGACCCCCACCCAACTTGCTTCGTTTTGCCCAGGGTTCCAT‑GCCCCC‑CGCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATa < 2:38645/147‑1 (MQ=255) aTCGACCACTTCGCAGACACCCCCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCACCTTGCTTCGTTTTGCGCAAGGGTCCAT‑GCCCCC‑CGCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAg < 1:671857/151‑1 (MQ=255) aTCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCAT‑GCCCCC‑CGCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTc > 2:715226/1‑149 (MQ=255) gACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCAT‑GCCCCC‑CGCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGAt > 1:22321/1‑151 (MQ=255) ccccTTCGCAGCCACCCCCCATATTGATGGCCTCGCGCGGCATGCCGAACCCCACGCACCTTGCTTCGTTTTGCGCAAGGGTCCAT‑GCCCCC‑CGCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGa < 2:152605/145‑1 (MQ=255) cTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCAT‑GCCCCC‑CGCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCAccc > 2:265320/1‑151 (MQ=255) cGCAGCCCCCACCCATATTGATGGCCTCGCGCGGCATGCCGAACCCCACGCAACTTTCTTCTTTTTGCGCAGGGGTCCAT‑GCCCCC‑CGCCTGACGCATCGCCACCATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCAccc < 2:408990/148‑1 (MQ=255) cAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCCCGCAACTTGCTTCGTTTTGCGCAAGGGTCCAT‑GCCCCC‑CGCCTGACGCATCGCAAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCAccc < 1:100452/146‑1 (MQ=255) gcccccccccATATGGATGCCCTCGCGCGGCATGCCAAACCCCACGAACCTTGCTTCGTTTTGCGCAAGGGTCCAT‑GCCCCC‑CGCCTGACGCATCCCCAACATCCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAAcc < 2:523237/142‑1 (MQ=255) ccccccATATTGATGGCCTCGCGCGGCATGCCGAACCCCACGCAACTTGCTTCGTTTTGCGCAGGGGCCCAT‑GCCCCC‑CGCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCAtt < 2:382077/147‑1 (MQ=255) accCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCAT‑GTCCCC‑CGCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTAc > 2:318815/1‑150 (MQ=255) cccATATTGATGGCCTAGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCAT‑GCCCCC‑CGCCTGACGCTTCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCCGGATCACCCCAACCGCATTACGc > 2:551727/1‑151 (MQ=255) cATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCAT‑GCCCCC‑CGCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGccc > 2:266836/1‑151 (MQ=255) ttGATGCCCTCCCGCGGCATGCCGACCACCCCGCAACTTGCTTCGTTTGGCGCAAGGGTCCAT‑GCCCCC‑CGCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCg < 2:624896/149‑1 (MQ=255) gATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCAT‑GCCCCC‑CGCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTg > 1:111736/1‑151 (MQ=255) gcgcgGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCAT‑GCCCCC‑CGCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGAc > 1:590366/1‑151 (MQ=255) acccccccGCACCTTCCTCCGTTTTGCGCAGGGGCCCAT‑GCCCCC‑CGCCTGACGCACCCCCAACTTCCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGaacaa < 2:30772/143‑1 (MQ=255) ccacGCAACTTGCTTCGTTTTGCGCAAGGGTCCAT‑GCCCCC‑CGCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACAt > 2:125122/1‑151 (MQ=255) cccccAATTTGCTCCTTTTTGCCCAGGGGCCCATCC‑CCCC‑CCCCTGCCCCTCCGCCAACATCACCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATc < 2:303520/147‑1 (MQ=255) aaCTTGCTTCGTTTTGCGCAAGGGTCCAT‑GCCCCC‑CGCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTa < 1:380117/148‑1 (MQ=255) aaCTTGCTTCGTTTTGCGCAAGGGTCCAT‑GCCCCC‑CGCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCg > 1:254646/1‑151 (MQ=255) cgtgctgcGTTTTGCGCAAGGGCCCAT‑GCCCCC‑CGCCTGACCCATGGCCACCATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTAc < 2:839153/145‑1 (MQ=255) tGCTTCGTTTTGCGCAAGGGTCCAT‑GCCCCC‑CGCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTGCGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCGAAgg > 2:236482/1‑151 (MQ=255) cTTCGTTTTGCGCAAGGGTCCAT‑GCCCCC‑CGCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCGAAGGcc > 1:885532/1‑151 (MQ=255) ttttGCGCAGGGGCCCATCC‑CCCC‑CGCTTGCCGCACCCCCAACATCCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCGAAGGCCg < 2:188979/147‑1 (MQ=255) ttttGCGCACGGGCCCAG‑C‑CCCCGCGCCTCCCGCAGCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATAACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCGAAg < 2:370654/143‑1 (MQ=255) tttgccgcAGGGGCCCAG‑GCCCCC‑CCCCTGACGCACCCCAACCATCCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCGAAGGCCGAt < 2:694995/144‑1 (MQ=255) gcgcAAGGGTCCAT‑GCCCCC‑CGCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCGAAGGCCGATGACGGt > 1:479021/1‑151 (MQ=255) | ACGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCAT‑G‑CCCC‑CGCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCGAAGGCCGATGACGGT > NC_000913/1967458‑1967741 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |