| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,049,805 | (T)5→6 | coding (863/1095 nt) | gcvT ← | aminomethyltransferase, tetrahydrofolate‑dependent, subunit (T protein) of glycine cleavage complex |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,049,800 | 1 | . | T | 97.8% | 174.1 / ‑3.2 | 46 | coding (868/1095 nt) | gcvT | aminomethyltransferase, tetrahydrofolate‑dependent, subunit (T protein) of glycine cleavage complex |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base T (20/25); total (20/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01 | |||||||||||
CGCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCC‑TTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTT > NC_000913/3049654‑3049943 | cGCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCC‑tttt < 2:93548/151‑1 (MQ=255)cGCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCC‑tttt < 1:383226/151‑1 (MQ=255) gCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCC‑ttttt < 2:287675/151‑1 (MQ=255) gTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCC‑ttttttcg < 2:179553/151‑4 (MQ=255) ttCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCC‑ttttttcgg < 1:197330/150‑5 (MQ=255) cGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGtc < 1:650941/150‑1 (MQ=255) aCCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAg < 1:606000/151‑1 (MQ=255) cTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGac > 1:195514/1‑151 (MQ=255) tCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGac < 1:393383/149‑1 (MQ=255) cgcCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGttttt > 2:857965/1‑150 (MQ=255) gcCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCt < 2:757815/151‑1 (MQ=255) cAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGt > 1:235053/1‑151 (MQ=255) cAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGt > 1:388237/1‑151 (MQ=255) cgcAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTAcc > 2:601903/1‑151 (MQ=255) cAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCAt > 2:887966/1‑151 (MQ=255) aaTGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATg > 1:288025/1‑151 (MQ=255) gCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCt < 1:794037/150‑1 (MQ=255) gTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCAc < 1:345001/150‑1 (MQ=255) gTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACg < 1:619397/151‑1 (MQ=255) ggggAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAgg < 1:905142/151‑1 (MQ=255) gggAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAggg < 1:322993/151‑1 (MQ=255) gggAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCC‑TTTTTCCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAggg < 1:599819/151‑1 (MQ=255) ggAGAAAGTGCCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCGCCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGc < 2:133680/151‑1 (MQ=255) gagaAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCt > 1:890025/1‑151 (MQ=255) agaAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCtt < 1:764251/151‑1 (MQ=255) gCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGAt > 1:671443/1‑151 (MQ=255) ggTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATaaa < 1:179397/151‑1 (MQ=255) gATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGt < 2:657216/150‑1 (MQ=255) aTAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCa < 2:697994/151‑1 (MQ=255) aTGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCgg > 2:53891/1‑151 (MQ=255) ttGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTccc > 2:58482/1‑149 (MQ=255) gCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGc > 1:667320/1‑151 (MQ=255) tGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCAACACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATg > 2:664192/1‑151 (MQ=255) gcATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTc > 2:196057/1‑151 (MQ=255) aTCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTcc > 2:124770/1‑150 (MQ=255) gTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGccc > 1:234982/1‑151 (MQ=255) aaGCGTACCGGCATTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGGCCATg < 2:788370/151‑1 (MQ=255) aaGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATg > 1:352737/1‑151 (MQ=255) tACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTggc > 1:187642/1‑151 (MQ=255) ccGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCGGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTggc < 1:8128/149‑1 (MQ=255) cATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGga < 1:20112/150‑1 (MQ=255) cATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGga < 1:61163/150‑1 (MQ=255) cATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGga < 2:67010/150‑1 (MQ=255) cATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCACGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGgag < 2:356810/151‑1 (MQ=255) aTTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGgaga < 1:803695/151‑1 (MQ=255) aCGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGgagaga < 1:352091/150‑1 (MQ=255) cagcaCGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGt > 2:55237/1‑151 (MQ=255) cagcaCGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGt < 1:132352/151‑1 (MQ=255) agcaCGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATggct > 2:885021/1‑149 (MQ=255) agcaCGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGtt < 1:767371/151‑1 (MQ=255) gcaCGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGttt < 2:592179/151‑1 (MQ=255) | CGCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCC‑TTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTT > NC_000913/3049654‑3049943 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |